The relationship between cell survival rate and telomerase activity of the human hepatocellular cells and carcinoma cells irradiated by X-rays

LIU Jinting; DANG Bingrong; LI Wenjian; JING Xigang; WEI Wei; WANG Zhuanzi; DING Nan; GAO Qingxiang

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The relationship between cell survival rate and telomerase activity of the human hepatocellular cells and carcinoma cells irradiated by X-rays

更新时间：2023-03-30

- The relationship between cell survival rate and telomerase activity of the human hepatocellular cells and carcinoma cells irradiated by X-rays
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 2, Pages: 119-123(2010)
- 作者机构：
  
  1.中国科学院近代物理研究所　兰州　730000
  2.中国科学院研究生院　北京　100049
  3.兰州大学生命科学院　兰州　730000
- 作者简介：
- 基金信息：
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LIU Jinting, DANG Bingrong, LI Wenjian, et al. The relationship between cell survival rate and telomerase activity of the human hepatocellular cells and carcinoma cells irradiated by X-rays. [J]. Journal of Radiation Research and Radiation Processing 28(2):119-123(2010)
DOI：

LIU Jinting, DANG Bingrong, LI Wenjian, et al. The relationship between cell survival rate and telomerase activity of the human hepatocellular cells and carcinoma cells irradiated by X-rays. [J]. Journal of Radiation Research and Radiation Processing 28(2):119-123(2010) DOI：

摘要

利用X射线对体外培养的人肝癌细胞SMMC-7721和正常人肝细胞HL-7702进行辐照，以细胞克隆形成法测定细胞存活率，多聚酶链式反应银染端粒重复序列扩增法（Telomeric repeat amplification protocol, TRAP-PCR）检测细胞中端粒酶活性变化，探讨辐照后细胞存活与细胞中端粒酶活性变化的关系。结果表明，SMMC-7721和HL-7702细胞的存活率都随辐照剂量的增加而相应降低。在1—4 Gy剂量范围内，两种细胞的端粒酶活性变化均呈剂量依赖性增强。但在1 Gy、2 Gy、3 Gy剂量下，SMMC-7721其端粒酶活性较对照（0 Gy）细胞低；当辐照剂量达4 Gy时，端粒酶活性略高于对照细胞。在1—4 Gy剂量范围内，HL-7702和SMMC-7721的细胞存活与端粒酶活性在变化趋势上呈负的关系。

Abstract

In this paper, the relationship between cell survival fraction and telomerase activity was investigated with the human hepatocellular cells HL-7702 and carcinoma cells SMMC-7721 irradiated by X-rays. The colony formation assay and TRAP (Telomeric repeat amplification protocol) method were used to determine the cell survival fraction and telomerase activity respectively. To both of HL-7702 and SMMC-7721, the telomerase activity was increased in dose-dependent. However, at 1—3 Gy, the telomerase activity was lower than that of control in SMMC-7721, and it was a little higher than that of control when irradiated at 4 Gy. At 1—4 Gy, the variation trend of cell survive and that of telomerase activity were identical.

关键词

X射线细胞存活率端粒酶活性SMMC-7721HL-7702

Keywords

X-raysCell survival rateTelomease activitySMMC-7721HL-7702

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