Biological effects of 2450 MHz microwave combined with γ-rays on rat cultured gliacytes

LU Minxia; NIE jihua; ZHU Jianquan; QIAN Cheng; WANG Guohai; TONG Jian

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Biological effects of 2450 MHz microwave combined with γ-rays on rat cultured gliacytes

更新时间：2023-03-30

- Biological effects of 2450 MHz microwave combined with γ-rays on rat cultured gliacytes
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 3, Pages: 172-176(2010)
- 作者机构：
  
  1.常州市疾病预防控制中心　常州　213022
  2.苏州大学放射医学与公共卫生学院卫生毒理教研室　苏州　215123
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LU Minxia, NIE jihua, ZHU Jianquan, et al. Biological effects of 2450 MHz microwave combined with γ-rays on rat cultured gliacytes. [J]. Journal of Radiation Research and Radiation Processing 28(3):172-176(2010)
DOI：

LU Minxia, NIE jihua, ZHU Jianquan, et al. Biological effects of 2450 MHz microwave combined with γ-rays on rat cultured gliacytes. [J]. Journal of Radiation Research and Radiation Processing 28(3):172-176(2010) DOI：

摘要

探讨微波及微波联合γ射线照射对大鼠神经胶质细胞凋亡率、线粒体膜电位（MMP）、胞内游离Ca,2+,和Ca,2+,-Mg,2+,-ATP酶活性的影响。将原代神经胶质细胞分为对照组(C)、微波组（M）、电离组（I）和联合组（IM）。M和IM组接受4 mW/cm,2,的微波辐射3 d，2 h/d；第4天，I和IM组接受5 Gy 1 Gy/min）的γ射线照射。采用Ca,2+,-Mg,2+,-ATP酶测试盒检测胞内Ca,2+,-Mg,2+,-ATP酶；流式细胞术检测细胞凋亡和MMP；流式细胞术和共聚焦激光扫描显微镜检测胞内游离Ca,2+,变化。结果发现，与C组相比，I和IM组细胞的凋亡率显著升高，各组细胞MMP未见明显变化；M、I和IM组胞内游离Ca,2+,明显升高，Ca,2+,-Mg,2+,-ATP酶活性显著降低，差异均有统计学意义(,p,<,0.05)。结果提示，在本试验条件下，2450 MHz微波可使胞内Ca,2+,显著上升，Ca,2+,-Mg,2+,-ATP酶活性显著降低；未观察到微波与γ射线照射大鼠神经胶质细胞的联合作用。

Abstract

To explore the biological effects of microwave combined with γ-rays on apoptosis, mitochondrion membrane potential(MMP), Ca,2+,and Ca,2+,-Mg,2+,-ATPase in rat cultured gliacytes, primarily cultured gliacytes were randomly divided into the control group(C), the microwave group(M), the γ-rays group(I) and the combined group (IM). The M and IM groups were exposed to 4 mW/cm,2, microwave 2h/d for 3 days. The I and IM groups were given 5 Gy ,60,Co γ-rays on the forth day. Ca,2+,-Mg,2+,-ATPase was detected by kit. Apoptosis, MMP and Ca,2+, were detected by flow cytometry(FCM). The results show that the ratio of apoptosis of the I and IM groups were significantly increased, and the contents of Ca,2+, in the cytoplasm of M, I and IM group cells were all significantly higher meanwhile Ca,2+,-Mg,2+,-ATPase activity was lower than that of the control group; but MMP had no obvious changes in the four groups. Under the experimental conditions, the microwave radiation did not induce evident changes in MMP. However, the content of Ca,2+, in the cytoplasm increased significantly and Ca,2+,-Mg,2+,-ATPase decreased obviously. No synergic effects were observed in the treatment with microwave before exposure to γ-rays.

关键词

微波γ射线神经胶质细胞凋亡MMPCa2+；Ca2+-Mg2+-ATP酶

Keywords

Microwaveγ-raysApoptosisCultured gliacytesMMPCa2+Ca2+-Mg2+-ATPase

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