In order to observe the effects computer radiation(CR) on learning and memory ability as well as cerebra neuron transmitter of mice, the 32 male Kunming mice were randomly divided into four groups as one control group and three CR groups irradiated for 6 h/d, 12 h/d, and 18 h/d, respectively. The three CR groups were settled 20cm before the operating computer (0.9 V/m) for 30 days consecutively. And the control group was placed in the environment without computer radiation (0 V/m). The learning and memory ability of the mice were measured by Y-type maze test at thirty-first day, then the activity of acetylcholinesterase (AchE), nitric oxide synthase (NOS) and glutamine synthetase (GS) were measured as well as the content of acetylcholine(Ach), nitric oxide(NO) and Glutamate (Glu). The results show that the content of Ach in cerebra decreased, but the content of NO and Glu were just the opposite; the activities of AchE and NOS were enhanced, but the activity of GS was just the opposite. It is revealed that the computer radiation can reduce the learning and memory ability of mice, which may involve the changes of cerebra neuron transmitter.