Study on relevance between kras gene and thymic lymphomas of miceinduced by X-rays

YU Lei; SUN Shilong; FANG Fang; LIU Yongzhe; GONG Hongwei; SHI Lei; JU Guizhi

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Study on relevance between kras gene and thymic lymphomas of miceinduced by X-rays

更新时间：2023-03-30

- Study on relevance between kras gene and thymic lymphomas of miceinduced by X-rays
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 3, Pages: 190-192(2010)
- 作者机构：
  
  1.吉林大学公共卫生学院　长春　130021
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YU Lei, SUN Shilong, FANG Fang, et al. Study on relevance between kras gene and thymic lymphomas of miceinduced by X-rays. [J]. Journal of Radiation Research and Radiation Processing 28(3):190-192(2010)
DOI：

YU Lei, SUN Shilong, FANG Fang, et al. Study on relevance between kras gene and thymic lymphomas of miceinduced by X-rays. [J]. Journal of Radiation Research and Radiation Processing 28(3):190-192(2010) DOI：

摘要

为探讨kras基因与辐射诱发小鼠胸腺淋巴瘤的关系，先采用X射线照射BALB/c小鼠建立胸腺淋巴瘤模型，取胸腺淋巴瘤和正常胸腺，分别提取总RNA、合成cDNA和提取总蛋白，采用实时定量PCR (Quantitative real-time PCR, QRT-PCR)和Western blot法对kras基因mRNA和蛋白表达分别进行检测。结果表明，胸腺淋巴瘤kras基因mRNA表达水平（2.66 ± 1.51）比正常胸腺组织（0.93 ± 0.30）明显增高，两组比较有统计学意义（,p,<,0.05），胸腺淋巴瘤kras基因蛋白表达水平也高于正常胸腺组织。结果提示，kras基因与辐射诱发小鼠胸腺淋巴瘤密切相关，可能是辐射致癌的一种易感基因。

Abstract

In order to investigate the relation between kras gene and thymic lymphomas of mice induced by X-rays, we made the thymic lymphomas model of BALB/c mice exposed to X-rays, so as to extracte total RNA and synthesize cDNA and total protein from both of thymic lymphomas and normal thymus tissue. The mRNA and protein expression of kras gene in thymic lymphomas and normal thymus were detected by QRT-PCR and Western blot, respectively. The results show that mRNA expression of kras gene in thymic lymphomas (2.66 ± 1.51) is significantly higher (,p,<,0.05) than that of normal thymus tissue (0.93±0.30); and protein expression of kras gene in thymic lymphomas is higher than that of normal thymus tissue. The results suggest that kras gene might be relative to thymic lymphomas induced by X-rays, and it might be a susceptibility gene to radiation carcinogenesis.

关键词

胸腺淋巴瘤kras基因电离辐射实时定量PCR

Keywords

Thymic lymphomasKras genesIonizing radiationQuantitative real-time PCR

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