Study on autophagy phenomenon in U251 cells irradiated by X-rays

CHEN Liesong; LI Jun; LONG Linmei; LIANG Zhongqin

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Study on autophagy phenomenon in U251 cells irradiated by X-rays

更新时间：2023-03-30

- Study on autophagy phenomenon in U251 cells irradiated by X-rays
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 4, Pages: 249-253(2010)
- 作者机构：
  
  1.苏州大学附属第二医院放疗科　苏州　215004
  2.苏州大学医学部药理学系　苏州　215123
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CHEN Liesong, LI Jun, LONG Linmei, et al. Study on autophagy phenomenon in U251 cells irradiated by X-rays. [J]. Journal of Radiation Research and Radiation Processing 28(4):249-253(2010)
DOI：

CHEN Liesong, LI Jun, LONG Linmei, et al. Study on autophagy phenomenon in U251 cells irradiated by X-rays. [J]. Journal of Radiation Research and Radiation Processing 28(4):249-253(2010) DOI：

摘要

为探讨自噬在X射线对U251细胞增殖抑制的作用及机制，用X射线处理人胶质瘤细胞系U251细胞，采用WST-1法测定X射线对U251细胞增殖的抑制作用，MDC荧光染色和LC3免疫组化观察辐照后自噬的发生；WST-1法检测3-MA对辐照诱导的U251细胞毒性的影响。WST-1法显示，X射线对U251细胞生长有显著的抑制作用，且此作用呈明显的时间、剂量依赖性；MDC荧光染色和LC3免疫组化。结果表明，X射线可诱导U251细胞发生自噬；X射线照射前阻断自噬使细胞毒性增强。X射线照射能明显抑制U251细胞的生长，并诱导其发生自噬，但自噬对U251细胞可能起到一定的保护作用。

Abstract

In order to investigate the effects of autophagy on proliferation inhibition in human U251 glioma cells irradiated by X-rays, WST-1 colorimetric assay, MDC staining and LC3 Immunohistochemistry were used. It has been found that X-rays can decrease the viability of U251 cells in a dose- and time-dependent manner by WST-1 colorimetric assay and induce autophagy in U251 cells confirmed by MDC staining and LC3 immunoreactivty. The results showed that X-rays could inhibit the proliferation significantly and induced autophagy of U251 cells.

关键词

X射线U251细胞自噬3-MA自噬束泡

Keywords

X-raysU251 cellsAutophagy3-MAAutophagic vacuoles (AVs)

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