Detection of the expression changes of human mitochondrial Cyt b, ATPase 6 and ATPase 8 genes induced by <sup>60</sup>Co γ-rays

LI Yuwen; FENG Jiangbin; LU Xue; FAN Li; CHEN Deqing; LIU Qingjie

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Detection of the expression changes of human mitochondrial Cyt b, ATPase 6 and ATPase 8 genes induced by ⁶⁰Co γ-rays

更新时间：2023-03-30

- Detection of the expression changes of human mitochondrial Cyt b, ATPase 6 and ATPase 8 genes induced by ⁶⁰Co γ-rays
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 4, Pages: 245-248(2010)
- 作者机构：
  
  1.中国疾病预防控制中心辐射防护与核安全医学所　北京　100088
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LI Yuwen, FENG Jiangbin, LU Xue, et al. Detection of the expression changes of human mitochondrial Cyt b, ATPase 6 and ATPase 8 genes induced by ⁶⁰Co γ-rays. [J]. Journal of Radiation Research and Radiation Processing 28(4):245-248(2010)
DOI：

LI Yuwen, FENG Jiangbin, LU Xue, et al. Detection of the expression changes of human mitochondrial Cyt b, ATPase 6 and ATPase 8 genes induced by ⁶⁰Co γ-rays. [J]. Journal of Radiation Research and Radiation Processing 28(4):245-248(2010) DOI：

摘要

用实时荧光定量PCR（Real-time PCR）探讨了,60,Co γ射线诱导人淋巴细胞线粒体Cyt b、ATPase6和ATPase8基因表达的改变。以1、3、5、8和10 Gy ,60,Co γ射线分别照射指数增长期的人淋巴细胞永生化细胞株，8 h后利用逆转录PCR（RT-PCR）摸索各基因的最佳实验条件，进而利用Real-time PCR的方法定量检测各基因表达变化，观察辐射对Cyt b、ATPase6和ATPase8基因表达影响的量效关系；同时以5 Gy ,60,Co γ射线照射人淋巴细胞后，分别于不同时点（0.5、4、8、12、24、48和72 h），通过RT-PCR和Real-time PCR方法检测各基因表达的变化，观察其时效关系。结果发现，在mRNA水平上，线粒体Cyt b、ATPase6和ATPase8基因表达总体上调。Cyt b和ATPase8基因在8 Gy剂量范围内，呈现出随剂量增加基因表达增强的量效关系趋势；观察时效关系，发现5 Gy剂量照射后4 h左右，3种基因表达增强最显著，且Cyt b和ATPase8基因分别持续高表达至48 h和72 h。因此，,60,Co γ射线可以诱导人淋巴细胞线粒体Cyt b、ATPase6和ATPase8基因表达的改变，总体上呈现表达增强趋势。

Abstract

This study is to explore the changes of human mitochondrial Cyt b, ATPase 6 and ATPase 8 genes expression induced by ,60,Co γ-rays irradiation. The dose-effect relationships between expression changes of the genes and the absorbed doses were analyzed by RT-PCR (Reverse Transcription PCR) and Real-time PCR at 8 h after the irradiation in human lymphoblastoid cell lines, which were exposed to 1—10 Gy ,60,Co γ-rays. The changes of these genes expression were also analyzed at different post-radiation time-points between 0.5 h and 72 h after irradiation of 5 Gy to explore the time-effect relationships. The results showed that the expression of 3 genes were up-regulated comprehensively. A good dose-effect relationship was showed for Cyt b and ATPase 8 at dose range of 0—8 Gy. For the time-effect after irradiation, the expression levels of 3 genes were up-regulated generally and the peak change time-point was 4 h after irradiation. ,60,Co γ-rays irradiation might induce the changes of mitochondrial Cyt b, ATPase 6 and ATPase 8 genes expression, and these genes expression are up-regulated generally.

关键词

60Co γ射线线粒体Cyt bATPase基因表达

Keywords

60Co γ-raysMitochondrionCyt bATPaseGene expression

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Detection of the expression changes of human mitochondrial Cyt b, ATPase 6 and ATPase 8 genes induced by 60Co γ-rays

DOI：

摘要

Abstract

关键词

Keywords

references

Detection of the expression changes of human mitochondrial Cyt b, ATPase 6 and ATPase 8 genes induced by ⁶⁰Co γ-rays