Effect of calcium-binding protein S100A8 in macrophage cell line RAW264.7 of mouse injured by irradiation

CONG Yue; RAO Yalan; CHEN Xiaohua; DONG Bo; LI Fengsheng; WANG Zhingdong; LUO Qingliang

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Effect of calcium-binding protein S100A8 in macrophage cell line RAW264.7 of mouse injured by irradiation

更新时间：2023-03-30

- Effect of calcium-binding protein S100A8 in macrophage cell line RAW264.7 of mouse injured by irradiation
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 5, Pages: 267-270(2010)
- 作者机构：
  
  1.军事医学科学院放射与辐射医学研究所　北京　100850
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CONG Yue, RAO Yalan, CHEN Xiaohua, et al. Effect of calcium-binding protein S100A8 in macrophage cell line RAW264.7 of mouse injured by irradiation. [J]. Journal of Radiation Research and Radiation Processing 28(5):267-270(2010)
DOI：

CONG Yue, RAO Yalan, CHEN Xiaohua, et al. Effect of calcium-binding protein S100A8 in macrophage cell line RAW264.7 of mouse injured by irradiation. [J]. Journal of Radiation Research and Radiation Processing 28(5):267-270(2010) DOI：

摘要

为探索钙结合蛋白S100A8在γ射线对造血免疫系统损伤中的作用，构建S100A8真核表达载体，转染小鼠巨噬细胞RAW264.7，研究S100A8在γ射线诱导RAW264.7细胞周期紊乱和胞内活性氧改变中的作用。运用RT-PCR和细胞荧光免疫方法鉴定增强表达S100A8 的RAW264.7 稳定细胞克隆。流式技术检测10 Gy γ射线照射6 h后细胞周期及H,2,O,2,刺激后胞内活性氧的变化。结果显示，与转染空质粒的对照细胞相比，增强表达S100A8细胞克隆G,0,/G,1,期细胞比例增高，S期细胞减少，不发生G,2,/M阻滞；并且H,2,O,2,作用后细胞中活性氧水平低于转染空白质粒的细胞。因此，增强表达S100A8的RAW264.7细胞与转染空白质粒细胞相比对辐射更具抗性，其可能原因是与增强表达的S100A8分子具有抗氧化功能有关。

Abstract

In order to establish the stable clones of RAW264.7 cells with S100A8 gene overexpression, and directly investigate the role of S100A8 in γ-rays induced hematoiesis/immune system injury, RT-PCR method and immuno-fluorescence staining analysis were performed to measure the expression of S100A8 mRNA and protein. The cell cycle was detected by flow cytometry at 6 h after irradiation with 10 Gy γ-rays. The level of reactive oxygen intermediates (ROIs) was detected by flow cytometry at 6h after H,2,O,2 ,treatment. The cell cycle analysis showed that the percentages of RAW264.7/S100A8 cells had an increase in G,0,/G,1, phase and a decrease in S phase, and G,2,/M arrest didn’t occur in experimental group while the level of ROIs was lower than that of the negative control cells. These results indicate that radiation resistant of RAW264.7/S100A8 cells may be related to the antioxidant role of over-expressed S100A8 in comparison to RAW264.7/pcDNA3.1+ cells.

关键词

S100A8γ射线细胞周期活性氧

Keywords

S100A8γ-raysCell cycleROIs

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