Radiation protective property of water-soluble chitosan with low-molecular weight

SANG Ru; WAN Jianmei; SU Xiaojun; ZHU Nankang; NING Ping; ZHANG Youjiu

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Radiation protective property of water-soluble chitosan with low-molecular weight

更新时间：2023-03-30

- Radiation protective property of water-soluble chitosan with low-molecular weight
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 5, Pages: 287-293(2010)
- 作者机构：
  
  1.苏州大学医学部放射医学与公共卫生学院　江苏省放射医学与防护重点实验室　苏州　215123
  2.苏州大学卫生与环境技术研究所　苏州　215123
  3.苏州中核华东辐照有限公司　苏州　215200
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SANG Ru, WAN Jianmei, SU Xiaojun, et al. Radiation protective property of water-soluble chitosan with low-molecular weight. [J]. Journal of Radiation Research and Radiation Processing 28(5):287-293(2010)
DOI：

SANG Ru, WAN Jianmei, SU Xiaojun, et al. Radiation protective property of water-soluble chitosan with low-molecular weight. [J]. Journal of Radiation Research and Radiation Processing 28(5):287-293(2010) DOI：

摘要

为研究水溶性低分子量壳聚糖（β-1, 4-2-氨基-2-脱氧-D-葡聚糖；Chitosan, CTS）的辐射保护作用，通过强碱洗脱法和乙酸-H,2,O,2,氧化降解法制备水溶性低分子量壳聚糖，采用四甲基偶氮噻唑蓝（MTT）法和流式细胞分析法分别检测低分子量壳聚糖对,60,Co γ射线照射后大鼠正常肝细胞（BRL）的存活率、细胞凋亡和细胞周期的影响，评价水溶性低分子量壳聚糖的辐射保护作用。结果表明，成功地制备了水溶性低分子量壳聚糖，壳聚糖的脱乙酰度由原来的79.3%提高到94.1%，乙酸-H,2,O,2,法降解120 min，壳聚糖的分子量由原来的8.50×10,5,下降到3.26×10,3,。低分子量壳聚糖（LMWC）对4 Gy ,60,Co γ射线照射后的BRL细胞具有良好的辐射保护作用，表现为照后细胞存活率明显升高，SubG1峰显著降低。50 μg/mL的CTS使4 Gy ,60,Co γ照射后48 h BRL细胞的存活率由66.5%±0.19% 提高到了87.5%±0.16%（,p,<,0.05）；照后24 h的SubG1峰由51.9%±2.1%降至16.9%±1.3%（,p,<,0.05）。表明制备的水溶性低分子量壳聚糖对BRL细胞有较好的辐射保护作用。

Abstract

In order to investigate the radioprotection property of water-soluble chitosan with low-molecular weight, sodium hydroxide eluting and oxidation degradation of chitosan with H,2,O,2, under HAc condition were used to prepare water-soluble low-molecular weight chitosan. MTT assay and flow cytometry (FCM) assay were performed to evaluate the cell survival rate, apoptosis and cell cycle alteration, respectively, after γ-rays irradiation. The results show that water-soluble low-molecular weight chitosan was successfully prepared, and the degree of deacetylation (D.D) of chitosan was increased from 79.3% to 94.1% while the molecular weight was decreased from 8.50×10,5, to 3.26×10,3, after treatment for 120 min. Low-molecular weight chitosan (LMWC) has good radio-protection effect to BRL cells from radiation damage induced by 4 Gy ,60,Co γ-rays in evidence of higher survival rate and lower SubG1 peek of the cells. After treatment with 50 μg/mL of LMWC, survival rate of BRL cells increased from 66.5%±0.19% to 87.5%±0.16% at 48 h (,p,<,0.05), the peak of SubG1 decreased from 51.9%±2.1% to 16.9%±1.3% at 24 h after 4 Gy ,60,Co γ-irradiation (,p,<,0.05).The results indicate that low molecular weight has excellent radio-protection effect to normal rat liver cell line (BRL).

关键词

壳聚糖低分子量脱乙酰度BRL细胞辐射防护

Keywords

ChitosanLow molecular weightDegree of deacetylation (D.D)BRL cellsRadiation protection

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