Strain improvement and optimization for β-glucosidase production in <italic style="font-style: italic">Aspergillus niger</italic> by low-energy N<sup>+</sup> implantation

DIAO Jinshan; WANG Li; CHEN Zhen; LIU Hui; NIE Guangjun; ZHENG Zhiming

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Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N⁺ implantation

更新时间：2023-03-30

- Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N⁺ implantation
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 6, Pages: 345-351(2010)
- 作者机构：
  
  1.中国科学院离子束生物工程学重点实验室　合肥　230031
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DIAO Jinshan, WANG Li, CHEN Zhen, et al. Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N⁺ implantation. [J]. Journal of Radiation Research and Radiation Processing 28(6):345-351(2010)
DOI：

DIAO Jinshan, WANG Li, CHEN Zhen, et al. Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N⁺ implantation. [J]. Journal of Radiation Research and Radiation Processing 28(6):345-351(2010) DOI：

摘要

为了获得β-葡萄糖苷酶高产菌株，应用低能N,+,注入技术对黑曲霉菌株Au进行了诱变选育，同时研究了N,+,注入对菌株存活率和突变率的影响。通过几轮注入之后，最终筛选得到的高产菌株Au 0847其β-葡萄糖苷酶活力达到13.75 U/mL，比原始菌株Au提高了106.8%，且遗传性能稳定。经过进一步的发酵优化，该菌株最终β-葡萄糖苷酶活力达到30.53 U/mL。

Abstract

Low-energy N,+, implantation was employed to mutate ,Aspergillus niger, Au to enhance productivity of β-glucosidase. Effects of N,+, on strains, survival and mutation rate were studied. After several rounds of implantation, activity of β-glucosidase of the final mutant Au 0847 reached 13.75 U/mL, which is higher by 106.8% than that of original strain Au, and its heritability was stabilized. Activity of β-glucosidase of Au 0847 reached 30.53 U/mL after further fermentation condition optimization.

关键词

β-葡萄糖苷酶离子注入黑曲霉选育发酵优化

Keywords

β-glucosidaseIon implantationAspergillus nigerBreedingFermentation optimization

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Tianjin Key Laboratory of Low Dimensional Materials Physics and Preparing Technology, Institute of Advanced Materials Physics

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Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N+ implantation

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摘要

Abstract

关键词

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Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N⁺ implantation