Silencing survivin gene enhances radio-sensitivity of human hepatoma SMMC-7721

YANG Wei; YIN Xiaoxiang; CAO Jianping; LIU Fenju

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Silencing survivin gene enhances radio-sensitivity of human hepatoma SMMC-7721

更新时间：2023-03-30

- Silencing survivin gene enhances radio-sensitivity of human hepatoma SMMC-7721
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 6, Pages: 352-356(2010)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院放射生物学教研室　苏州　215123
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YANG Wei, YIN Xiaoxiang, CAO Jianping, et al. Silencing survivin gene enhances radio-sensitivity of human hepatoma SMMC-7721. [J]. Journal of Radiation Research and Radiation Processing 28(6):352-356(2010)
DOI：

YANG Wei, YIN Xiaoxiang, CAO Jianping, et al. Silencing survivin gene enhances radio-sensitivity of human hepatoma SMMC-7721. [J]. Journal of Radiation Research and Radiation Processing 28(6):352-356(2010) DOI：

摘要

为探讨沉默生存素（Survivin）基因对人肝癌细胞SMMC-7721放射敏感性的影响，利用靶向Survivin基因的RNA干扰（RNA interference，RNAi）质粒pGenesil-survivin，采用阳离子脂质体介导法转染SMMC-7721细胞后48 h，分别以RT-PCR和Western blot法检测Survivin基因mRNA和蛋白表达，以四甲基偶氮唑盐（MTT）法检测细胞增殖活性，流式细胞术检测细胞周期和细胞凋亡，克隆存活实验检测细胞放射敏感性的变化。结果表明，转染质粒pGenesil-survivin后48 h，SMMC-7721细胞Survivin基因mRNA和蛋白表达水平与对照和阴性干扰组相比明显降低（,p,<,0.05）；转染后第1—4 d，细胞增殖活性与对照组相比明显降低（,p,<,0.05或,p,<,0.01）；转染后48 h，细胞阻滞于G,2,/M期（,p,<,0.001），凋亡百分率明显高于对照组（,p,<,0.001）；转染后SMMC-7721细胞,D,0,值减小，放射敏感性增强，增敏比为1.24。结果提示，沉默生存素基因可增强人肝癌细胞SMMC-7721放射敏感性。

Abstract

In order to investigate the effect of RNA interference silencing survivin gene on radio-sensitivity of human hepatoma SMMC-7721 cells, mRNA and protein expression of survivin gene in SMMC-7721 cells after transfection with pGenesil-survivin-HIF mediated by liposome were detected by RT-PCR and western blot respectively. Cell viability was detected by MTT assay. Cell cycle distribution and apoptosis of SMMC-7721 cells were detected by FCM assay. Radio-sensitivity of SMMC-7721 cells was determined by clonogenic assay. The results showed that mRNA and protein expression of survivin gene in SMMC-7721 cells was significantly lower than that of control and negative interference group 48 h after transfection with pGenesil-survivin (,p,<,0.05). Cell viability of pGenesil-survivin group was significantly lower than that of control group (,p,<,0.05 or ,P,<,0.01). Cells arrested in the G,2,/M phase of cell cycle (,p,<,0.001) and percentage of apoptosis was significantly higher than that of control group 48 h after transfection with pGenesil-survivin (,p,<,0.001). ,D,0, of SMMC-7721 cells after transfection with pGenesil-survivin decreased and its radio-sensitivity increased. The radio-sensitization ratio was determined to be 1.24. These results can be suggested that silencing survivin gene enhances radio-sensitivity of human hepatoma SMMC- 7721 cells.

关键词

RNA干扰生存素肝细胞癌放射敏感性

Keywords

RNA interferenceSurvivinHepatomaRadio-sensitivity

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1(Department of Radiobiology, School of Radiological Medicine and Public Health, Soochow University,,)

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