The effects of <sup>18</sup>F-FDG on the proliferation of HepG2 liver cancer cells

WANG Mingming; SI Yuan; QIU Jun; XIE Qiang; XIE Jikui

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The effects of ¹⁸F-FDG on the proliferation of HepG2 liver cancer cells

更新时间：2023-03-30

- The effects of ¹⁸F-FDG on the proliferation of HepG2 liver cancer cells
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 6, Pages: 357-362(2010)
- 作者机构：
  
  1.安徽医科大学核医学教研室　合肥　230032
  2.安徽省立医院PET-CT中心　合肥　230001
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WANG Mingming, SI Yuan, QIU Jun, et al. The effects of ¹⁸F-FDG on the proliferation of HepG2 liver cancer cells. [J]. Journal of Radiation Research and Radiation Processing 28(6):357-362(2010)
DOI：

WANG Mingming, SI Yuan, QIU Jun, et al. The effects of ¹⁸F-FDG on the proliferation of HepG2 liver cancer cells. [J]. Journal of Radiation Research and Radiation Processing 28(6):357-362(2010) DOI：

摘要

为研究,18,F-FDG对HepG2肝癌细胞增殖的影响，探讨其作用机制，以0—92.5×10,6, Bq/mL的,18,F-FDG作用HepG2肝癌细胞后6、12和24 h，用倒置显微镜观察、流式细胞术和逆转录-聚合酶链式反应（RT-PCR）技术检测细胞增殖、凋亡、活性氧含量及P53基因表达。结果表明，,18,F-FDG能诱导HepG2肝癌细胞的凋亡，并随,18,F-FDG放射性浓度的增大，细胞凋亡率增大，活性氧含量增加，P53表达增强。由此可见，,18,F-FDG能通过诱发HepG2肝癌细胞凋亡来抑制其增殖，且抑制率呈放射性浓度依赖性升高。

Abstract

The influence of ,18,F-FDG on the proliferation of HepG2 liver cancer cell was investigated and related possible mechanism was elucidated in this paper. HepG2 liver cancer cells were conventionally cultured. The cells during the phase of logarithmic growth were divided into different groups of ,18,F-FDG treatment and control groups. At 6,12 and 24h after treatment of 0—92.5×10,6,Bq/mL of ,18,F-FDG, inverted microscopy, the cell apoptosis was analyzed by use of flow cytometry and reverse transcription-polymerase chain reaction to analyze meanwhile the content of reactive oxygen species (ROS) and expression of p53 gene in cells induced by ,18,F-FDG, were determined respectively. After exposure to ,18,F-FDG, the apoptotic morphological changes of HepG2 liver cancer cells were observed. It was found that inhibition rate of cell proliferation, production of ROS and apoptosis rate increased with the radioactivity concentration of ,18,F-FDG in the range of 0—92.5×10,6,Bq/mLwhile the expression of p53 gene increased gradually. The results indicate that ,18,F-FDG may inhibit the proliferation of HepG2 liver cancer cells by apoptosis and increment of inhibition rate.

关键词

18氟-氟代脱氧葡萄糖HepG2肝癌细胞细胞增殖细胞凋亡

Keywords

18F-2-Deoxy-2-Fluoro-D-Glucose (18F-FDG)HepG2 liver cancer cellCells proliferationApoptosis

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Teaching and Research Department of Human Anatomy and Histology and Embryology, Medical Department of Qingdao University

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University of Chinese Academy of Sciences

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The effects of 18F-FDG on the proliferation of HepG2 liver cancer cells

DOI：

摘要

Abstract

关键词

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references

The effects of ¹⁸F-FDG on the proliferation of HepG2 liver cancer cells