Establishment of the dose-effect curve between Calyculin A induced premature chromosome condensation ring and exposed dose

LU Xue; ZHAO Hua; CHEN Deqing; FENG Jiangbin; LIU Qingjie; SU Xu

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Establishment of the dose-effect curve between Calyculin A induced premature chromosome condensation ring and exposed dose

更新时间：2023-03-30

- Establishment of the dose-effect curve between Calyculin A induced premature chromosome condensation ring and exposed dose
- Journal of Radiation Research and Radiation Processing Vol. 28, Issue 6, Pages: 363-367(2010)
- 作者机构：
  
  1.中国疾病预防控制中心辐射防护与核安全医学所　北京　100088
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LU Xue, ZHAO Hua, CHEN Deqing, et al. Establishment of the dose-effect curve between Calyculin A induced premature chromosome condensation ring and exposed dose. [J]. Journal of Radiation Research and Radiation Processing 28(6):363-367(2010)
DOI：

LU Xue, ZHAO Hua, CHEN Deqing, et al. Establishment of the dose-effect curve between Calyculin A induced premature chromosome condensation ring and exposed dose. [J]. Journal of Radiation Research and Radiation Processing 28(6):363-367(2010) DOI：

摘要

以Calyculin A诱导早熟染色体凝聚（PCC）方法，探索G,2,/M-PCC细胞中PCC环用于分析电离辐射损伤程度的可行性，用0—20 Gy（剂量率为1 Gy/min）的,60,Co γ 射线照射外周血，培养48 h并用Calyculin A诱导PCC。分析各剂量点G,2,/M-PCC指数、判断观察到的PCC环是否符合泊松分布，建立PCC环率与剂量之间的剂量效应曲线。结果发现，用Calyculin A可成功诱导人外周血淋巴细胞产生PCC，且G,2,/M-PCC指数随着剂量水平的增高而降低。除20 Gy剂量外，0—16 Gy各剂量点样品中PCC环的分布符合泊松分布。PCC环率随着剂量增加而增加，剂量-效应曲线可以拟合成,y,=0.0128,x,−0.0104直线方程，测得,R,2,=0.9898（,y,为PCC-R率，,x,为剂量）；二次多项式方程为,y,=0.00002,x,2,+ 0.0085,x,+0.0011 ,R,2,=0.9996。由此可见，Calyculin A诱导淋巴细胞G,2,/M-PCC中的PCC环率可用于生物体电离辐射损伤程度测定。

Abstract

In order to explore the probability of PCC-ring (PCC-R) in G,2,/M-PCC cells could be used to quantify the extent of ionizing radiation damage, premature chromosome condensation (PCC) induced by Calyculin A was analyzed. Peripheral blood samples were irradiated with 0, 4, 8, 12, 16 and 20 Gy ,60,Co γ-rays. The PCC-R, which was induced by Calyculin A after culture of 48 h, was observed in the samples exposed to different dose of ,60,Co γ-rays. The G,2,/M-PCC index and the distribution of PCC-R were calculated; the dose-response curves between the frequency of PCC-R and exposed dose level were established. The results showed that PCC was successfully induced by Calyculin A. The G,2,/M-PCC index decreased with exposed dose level. The PCC-R distribution followed a Poisson law for most of dose levels except 20 Gy. The frequency of PCC-R increased with the exposed dose level. And the dose-effect curve between the frequency of hollow-, kidney-shape ring and exposed dose level was fitted with two models, a linear model ,y,=0.0128x−0.0104 ,R,2,=0.9898 and a non-linear model ,y,=0.00002x,2,+0.0085x+0.0011 ,R,2,=0.9996. The frequency of PCC-R in peripheral blood lymphocyte G,2,/M-PCC cells induced by Calyculin A could be used to quantify the extent of ionizing radiation damage.

关键词

电离辐射淋巴细胞早熟染色体凝聚Calyculin APCC环

Keywords

Ionizing radiationLymphocytePremature chromosome condensationCalyculin APCC-ring

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