EFFECTS OF INHIBITORS OF SIGNALING MOLECULARS ON THE IRRADIATED NFS-60 CELLS

CONG Yuwen; CHEN Jiapei; ZHAO Weihong; WU Lanjun

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EFFECTS OF INHIBITORS OF SIGNALING MOLECULARS ON THE IRRADIATED NFS-60 CELLS

更新时间：2023-04-07

- EFFECTS OF INHIBITORS OF SIGNALING MOLECULARS ON THE IRRADIATED NFS-60 CELLS
- Journal of Radiation Research and Radiation Processing Vol. 18, Issue 3, Pages: 212-217(2000)
- 作者机构：
  
  1.北京放射医学研究所　北京　100850
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CONG Yuwen, CHEN Jiapei, ZHAO Weihong, et al. EFFECTS OF INHIBITORS OF SIGNALING MOLECULARS ON THE IRRADIATED NFS-60 CELLS. [J]. Journal of Radiation Research and Radiation Processing 18(3):212-217(2000)
DOI：

CONG Yuwen, CHEN Jiapei, ZHAO Weihong, et al. EFFECTS OF INHIBITORS OF SIGNALING MOLECULARS ON THE IRRADIATED NFS-60 CELLS. [J]. Journal of Radiation Research and Radiation Processing 18(3):212-217(2000) DOI：

摘要

为了解造血因子受体信号转导在造血细胞辐射损伤时的变化及其与细胞增殖的关系，应用MTT法分别观察了蛋白质酪氨酸和丝苏氨酸激酶及其磷酸酶抑制剂对照射及未照射NFS-60细胞增殖的影响。结果显示，一定浓度的磷酸酶抑制剂可促进细胞增殖，而激酶抑制剂则抑制细胞生长，同未照射细胞相比，磷酸酶抑制剂对3Gy照射细胞具有更强的促增殖作用，而某些激酶抑制剂则可加重细胞的辐射损伤。提示辐射后NFS-60细胞激酶／磷酸酶自稳平衡失调，推测这可能是辐射后造血细胞增殖抑制的原因之一。

Abstract

In order to study the change of receptor signal transduction after irradiation and its relation with growth of irradiated hematopoietic cells, the effects of the inhibitors of protein-tyrosine kinase and phosphatase on irradiated and unirradiated NFS-60 cells were observed by using MTT method. It was showed that the proliferation of NFS-60 cells was promoted by phosphatase inhibitors and suppressed by kinase inhibitors. Compared with unirradiated cells, phosphatase inhibitors had stronger stimulating effects on irradiated NFS-60 cells with 3Gy irradiation, meanwhile some kinase inhibitors might repress the growth of irradiated cells relatively. It was suggested that the homostasis of kinase and phosphatase were out of balance in irradiated NFS-60 cells, which might be one of the reasons for growth repression of hematopoietic cells after irradiation.

关键词

信号分子抑制剂辐射损伤NFS-60细胞

Keywords

Signaling molecular inhibitorsRadiation injuryNFS-60 cells

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