EFFECT OF IONIZING RADIATION ON p53 AND MDM2 GENE EXPRESSIONS OF EL-4 CELLS

FU Haiqing; LUO Can; FU Shibo; JU Guizhi

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EFFECT OF IONIZING RADIATION ON p53 AND MDM2 GENE EXPRESSIONS OF EL-4 CELLS

更新时间：2023-04-07

- EFFECT OF IONIZING RADIATION ON p53 AND MDM2 GENE EXPRESSIONS OF EL-4 CELLS
- Journal of Radiation Research and Radiation Processing Vol. 18, Issue 3, Pages: 208-211(2000)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物学重点实验室　长春　130021
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FU Haiqing, LUO Can, FU Shibo, et al. EFFECT OF IONIZING RADIATION ON p53 AND MDM2 GENE EXPRESSIONS OF EL-4 CELLS. [J]. Journal of Radiation Research and Radiation Processing 18(3):208-211(2000)
DOI：

FU Haiqing, LUO Can, FU Shibo, et al. EFFECT OF IONIZING RADIATION ON p53 AND MDM2 GENE EXPRESSIONS OF EL-4 CELLS. [J]. Journal of Radiation Research and Radiation Processing 18(3):208-211(2000) DOI：

摘要

采用流式细胞术和Northern blot杂交检测了X射线照射后EL-4细胞中p53蛋白及其下游基因MDM2 mRNA和蛋白表达的变化，以探讨X射线照射诱导EL-4细胞G,1,期阻滞的分子机制。结果表明：经4Gy X射线照射后的EL-4细胞，p53蛋白表达在照射后2h增高，4h达峰值，持续至照射后24h（,p,<,0.05～0.001）；MDM2蛋白的表达在照射后4～24h明显升高（,p,<,0.05～0.01）。进一步观察X射线照射后MDM2 mRNA水平的时程变化后发现，在经4Gy X射线照射后的EL-4细胞中，MDM2 mRNA水平在照射后l～8h有增高趋势，其中，2h明显升高（,p,<,0.01）。结果表明，X射线照射EL-4细胞可通过p53蛋白诱导MDM2蛋白表达，从而限制G,1,期阻滞的时间，使DNA损伤修复后的细胞重新进入细胞周期。

Abstract

To explore the molecular mechanism of G,1, arrest of EL-4 cells induced by whole body irradiation (WBI), Flow cytometry (FCM) and Northern blot were used to analyze p53, MDM2 gene expressions of EL-4 cells after irradiation with X-rays. Results showed that the p53 protein level of El-4 cells increased from 2 to 24h, and the MDM2 protein level of El-4 increased from 4 to 24h after 4Gy irradiation with X-rays. Meanwhile, the MDM2 mRNA level was significantly increased at 4h after irradiation. Conclusion: these results suggested that there was a feedback loop between p53 and MDM2 protein in EL-4 cells after irradiation with X-rays, which made G,1, arrest cells reenter cell cycle.

关键词

电离辐射EL-4细胞MDM2p53

Keywords

Ionizing radiationEL-4 cellsMDM2p53

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