INDUCTION AND REPAIR OF DNA DOUBLE-STRAND BREAKS IN HUMAN HEPATOMA CELLS IRRADIATED WITH <sup>16</sup>O<sup>6+</sup>

ZHOU Guangming; WEI Zengquan; LI Wenjian; WANG Jufang; HE Jing; LI Qiang; DANG Bingrong; XIE Hongmei; GAO Qingxiang

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INDUCTION AND REPAIR OF DNA DOUBLE-STRAND BREAKS IN HUMAN HEPATOMA CELLS IRRADIATED WITH ¹⁶O⁶⁺

更新时间：2023-04-07

- INDUCTION AND REPAIR OF DNA DOUBLE-STRAND BREAKS IN HUMAN HEPATOMA CELLS IRRADIATED WITH ¹⁶O⁶⁺
- Journal of Radiation Research and Radiation Processing Vol. 18, Issue 3, Pages: 198-202(2000)
- 作者机构：
  
  1.中国科学院近代物理研究所　兰州　730000
  2.兰州大学生物系　兰州　730000
- 作者简介：
- 基金信息：
  
  "Hope of the West"(XB980604)
- DOI：
  CLC：
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ZHOU Guangming, WEI Zengquan, LI Wenjian, et al. INDUCTION AND REPAIR OF DNA DOUBLE-STRAND BREAKS IN HUMAN HEPATOMA CELLS IRRADIATED WITH ¹⁶O⁶⁺. [J]. Journal of Radiation Research and Radiation Processing 18(3):198-202(2000)
DOI：

ZHOU Guangming, WEI Zengquan, LI Wenjian, et al. INDUCTION AND REPAIR OF DNA DOUBLE-STRAND BREAKS IN HUMAN HEPATOMA CELLS IRRADIATED WITH ¹⁶O⁶⁺. [J]. Journal of Radiation Research and Radiation Processing 18(3):198-202(2000) DOI：

摘要

用倒转脉冲场凝胶电泳（PIGE）研究了3.17MeV/u ,16,O,6+,诱导的肝癌细胞DNA双链断裂（DSB）及其修复效应。结果表明：DSB的诱导与辐照剂量呈正相关，其产额为0.43DSBs/100Mbp:Gy，与,60,Co γ射线相比相对生物学效应为1.69。DSB片段重接一半所需要的时间（,t,1/2,）与剂量有关，剂量越大，,t,1/2,越长。重接的方式主要表现为小片段连接为较大的片段。

Abstract

Pulsed-field gel electrophoresis has been applied to analyse the induction and repair of DNA double-strand breaks (DSB) in human hepatoma SMMC-7721 cells exposed to 3.17MeV/u ,16,O,6+,. It shows that the DSB yields are 0.43DSBs/100Mbp/Gy and compared with ,60,Co γ-rays, the relative biological effectiveness (RBE) of ,16,O,6+, is 1.69. The half time of DSB fragments being repaired (,t,1/2,) is relevant to the dose; the higher the dose is, the longer the time is. The main way for DSB repair is the rejoining of smaller fragments into larger ones.

关键词

16O6+DNA双链断裂脉冲场凝胶电泳人肝癌细胞

Keywords

16O6+DNA double-strand breaksPulsed field gel electrophoresisHuman hepatoma cells

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INDUCTION AND REPAIR OF DNA DOUBLE-STRAND BREAKS IN HUMAN HEPATOMA CELLS IRRADIATED WITH 16O6+

DOI：

摘要

Abstract

关键词

Keywords

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INDUCTION AND REPAIR OF DNA DOUBLE-STRAND BREAKS IN HUMAN HEPATOMA CELLS IRRADIATED WITH ¹⁶O⁶⁺