EFFECT OF X-RAYS ON CELL CYCLE PROGRESSION AND CELL DOUBLING TIME IN EL-4 LYMPHOMA CELLS

Ju Guizhi; Fu Shibo; Liu Jianxiang; Liu Shuzheng

您当前的位置：

首页 >

文章列表页 >

EFFECT OF X-RAYS ON CELL CYCLE PROGRESSION AND CELL DOUBLING TIME IN EL-4 LYMPHOMA CELLS

更新时间：2023-04-18

- EFFECT OF X-RAYS ON CELL CYCLE PROGRESSION AND CELL DOUBLING TIME IN EL-4 LYMPHOMA CELLS
- Journal of Radiation Research and Radiation Processing Vol. 16, Issue 3, Pages: 157-160(1998)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物实验室　长春　130021
- 作者简介：
- 基金信息：
  
  The National Natural Science Foundation of China
- DOI：
  CLC：
扫描看全文
Ju Guizhi, Fu Shibo, Liu Jianxiang, et al. EFFECT OF X-RAYS ON CELL CYCLE PROGRESSION AND CELL DOUBLING TIME IN EL-4 LYMPHOMA CELLS. [J]. Journal of Radiation Research and Radiation Processing 16(3):157-160(1998)
DOI：

Ju Guizhi, Fu Shibo, Liu Jianxiang, et al. EFFECT OF X-RAYS ON CELL CYCLE PROGRESSION AND CELL DOUBLING TIME IN EL-4 LYMPHOMA CELLS. [J]. Journal of Radiation Research and Radiation Processing 16(3):157-160(1998) DOI：

摘要

目的研究不同剂量X射线照射对EL-4淋巴瘤细胞周期进程及细胞倍增时间的影响。方法离体培养EL-4细胞，计算细胞倍增时间。PI荧光探针标记，流式细胞术（FCM）检测细胞周期各时相细胞的变化。结果0.5Gy～4.0Gy X射线照射使EL-4细胞倍增时间明显延长，G,1,及G,2,期细胞数明显增加。结论0.5Gy～4.0Gy X射线照射诱导EL-4细胞明显的G,1,及G,2,期细胞阻滞，此变化显示出明显的剂量效应和时间效应。细胞周期进程变化与细胞倍增时间延长密切相关。

Abstract

In the present study,the effect of X-rays on EL-4 cell doubling time and cell cycle progression were investigated. Cell doubling time in culture was calculated. Propidium iodide (PI) staining and flow cytometer (FCM) were used for cell cycle measurement. The results showed that EL-4 cell doubling time was prolonged significantly after irradiation with doses of 0.5, 1.0,2.0 or 4.0Gy. G,1, arrest occurred at 72h after 1.0Gy and at 12h to 72h after 2.0 or 4.0Gy. G,2, arrest occurred at 72h after 0.5 or 1.0Gy and at 48h to 72h after 2.0 or 4.0Gy. S phase cells decreased after 1.0,2.0 or 4.0Gy exposure. We concluded that 0.5 to 4. 0Gy X-irradiation could induce G,1, and G,2, arrest for EL-4 cells,which related closely to cell doubling time prolongation.

关键词

电离辐射G1期阻滞G2期阻滞细胞倍增时间

Keywords

X-irradiationG1 arrestG2 arrestCell doubling time

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Changes in p16 mRNA level and protein expression in thymocytes and splenocytes after whole body irradiation

EFFECT OF IONIZING RADIATION ON THE CDK4 EXPRESSION IN MOUSE BONE MARROW CELLS

G₁ ARREST AND THE EXPRESSIONS OF RELATIVE PROTEINS IN EL-4 CELLS INDUCED BY IONIZING RADIATION

THE ROLE OF p53 AND ITS DOWN-STREAM GENE IN G₁ ARREST OF MOUSE THYMOCYTES INDUCED BY IONIZING RADIATION

Related Author

No data

Related Institution

Shantou University Medical College

Radiobiology department, Public Health College, Jilin University

MH Radiobiology Research Unit, Norman Bethune University of Medical Science

MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences

⁰