EFFECTS OF 5-HYDROXYTRAPTAMINE ON GROWTH OF NORMAL AND IRRADIATED NIH/3T3 FIBROBLASTS

Cui Yufang; Yang Hong; Gao Yabing; Xiong Chengqi; Xia Guowei; Wang Dewen

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EFFECTS OF 5-HYDROXYTRAPTAMINE ON GROWTH OF NORMAL AND IRRADIATED NIH/3T3 FIBROBLASTS

更新时间：2023-04-18

- EFFECTS OF 5-HYDROXYTRAPTAMINE ON GROWTH OF NORMAL AND IRRADIATED NIH/3T3 FIBROBLASTS
- Journal of Radiation Research and Radiation Processing Vol. 16, Issue 3, Pages: 153-156(1998)
- 作者机构：
  
  1.军事医学科学院放射医学研究所　北京　100850
- 作者简介：
- 基金信息：
  
  The Science Foundation of PLA
- DOI：
  CLC：
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Cui Yufang, Yang Hong, Gao Yabing, et al. EFFECTS OF 5-HYDROXYTRAPTAMINE ON GROWTH OF NORMAL AND IRRADIATED NIH/3T3 FIBROBLASTS. [J]. Journal of Radiation Research and Radiation Processing 16(3):153-156(1998)
DOI：

Cui Yufang, Yang Hong, Gao Yabing, et al. EFFECTS OF 5-HYDROXYTRAPTAMINE ON GROWTH OF NORMAL AND IRRADIATED NIH/3T3 FIBROBLASTS. [J]. Journal of Radiation Research and Radiation Processing 16(3):153-156(1998) DOI：

摘要

用噻唑盐（MTT）比色、（AgNORs）银染和抗III型胶原及抗5-溴脱氧尿苷单抗免疫组化方法，观察5-羟色胺（5-hydroxytraptamine, 5-HT）对正常和受照NIH/3T3成纤维细胞生长的影响。结果表明，浓度为2.0×10-,6,mol/L的5-HT能使正常和受照射成纤维细胞数增至对照值的1.5倍；能使上述成纤维细胞的增殖活性（OD值）升高至对照值的1.3和1.6倍；还可使正常成纤维细胞AgNORs数量增加至对照值的2.5倍；此外浓度为1.0×10,-6,mol/L的5-HT可使S期成纤维细胞比例和III型胶原阳性细胞数明显增加，分别为对照值的2.7和2.1倍。

Abstract

Effects of 5-hydroxytraptamine (5-HT) on growth of normal and irradiated NIH/3T3 fibroblasts were observed by using MTT colorimetry,a silver colloid technique of AgNORs, and immunohistochemical methods were used to estimate type III collagen and DNA synthesis. The results showed that,when 5-HT of 2×10,-6,mol/L was used,the counts of normal and irradiated fibroblasts increased by 1.5 times as many as that of control group; proliferative activity (expressed by OD values) of the fibroblasts were about 1. 3 and 1.6 times that of control value; the number of AgNORs granules elevated to 2.5 times that of control value. In addition,5-HT of 1.0×10,-6,mol/L also induced the increments of percentage of S-phase fibroblasts and type III collagen positive fibroblasts,both of them were 2.7 and 2.1 times that of control group,respectively.

关键词

5-羟色胺成纤维细胞γ射线照射III型胶原DNA合成

Keywords

5-hydroxytraptamineFibroblastγ-IrradiationType III collagenDNA synthesis

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