CORRELATION BETWEEN THE INHIBITION OF DNA POLYMERASE β ACTIVITY IN IRRADIATED PLATEAU PHASE CELLS AND THE RADIOPOTENTIATION BY CM

Zheng Xiulong; Gao Jianguo; Yang Lixi

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CORRELATION BETWEEN THE INHIBITION OF DNA POLYMERASE β ACTIVITY IN IRRADIATED PLATEAU PHASE CELLS AND THE RADIOPOTENTIATION BY CM

更新时间：2023-04-23

- CORRELATION BETWEEN THE INHIBITION OF DNA POLYMERASE β ACTIVITY IN IRRADIATED PLATEAU PHASE CELLS AND THE RADIOPOTENTIATION BY CM
- Journal of Radiation Research and Radiation Processing Vol. 10, Issue 4, Pages: 198-202(1992)
- 作者机构：
  
  1.第二军医大学，上海　200433
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(3870889)
- DOI：
  CLC：
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Zheng Xiulong, Gao Jianguo, Yang Lixi. CORRELATION BETWEEN THE INHIBITION OF DNA POLYMERASE β ACTIVITY IN IRRADIATED PLATEAU PHASE CELLS AND THE RADIOPOTENTIATION BY CM. [J]. Journal of Radiation Research and Radiation Processing 10(4):198-202(1992)
DOI：

Zheng Xiulong, Gao Jianguo, Yang Lixi. CORRELATION BETWEEN THE INHIBITION OF DNA POLYMERASE β ACTIVITY IN IRRADIATED PLATEAU PHASE CELLS AND THE RADIOPOTENTIATION BY CM. [J]. Journal of Radiation Research and Radiation Processing 10(4):198-202(1992) DOI：

摘要

借助于DNA聚合酶,α,专一性抑制剂NEM，观察了CM对受12 Gy照射的相对密度生长期V,79,细胞核DNA聚合酶,β,活力的作用。结果表明：在α酶完全被抑制的条件下，除照后即刻CM对,β,酶活力无明显影响外（p,>,0.05），保温2-6 h均有抑制作用，其抑制程度随CM浓度和照后保温时间（4 h内）的增加而加强，抑制了DNA的修复，提高了辐射对DNA的损伤效应。其抑制规律，与在相同试验条件下，CM对受照相对密度生长期V,79,细胞潜在致死损伤修复（PLDR）的抑制规律基本相似，提示CM对PLDR的抑制作用，可能通过抑制DNA修复酶系中的,β,酶所造成，从而增强了射线对细胞的杀灭效应。

Abstract

By the aid of a specific DNA polymerase ,α, inhibiter NEM, the DNA polymerase ,β, activity of irradiated platcau phase V,79, cell nuclei was observed. The results showed: there was no influence on the polymerase ,β, activity of celi nuclei (p,>,0.05). when the cells were treated with 4 or 10 mmol/L CM immeciately following 12 Gy irradiation. However, CM could depress polymerase ,β, activity of cell nuclei evidently after 2-6 h incubation. The degree of inhibition was enhanced with the increasing CM dosage. and with the prolorgation of incubation time. It implied that CM could inhibit the DNA repair and enhance laciaticn-induced DNA danage. Its inhibition rule was similar to that of CM on the PLDR of plateau phase V,79, cells under same experimental conditions. It is suggested that the inhibition of PLDR by CM might be through its effeet on polymerase ,β, in DNA repair system, and then resulted in the enhancement of cell killing

关键词

电离辐射甲硝唑氨酸相对密度生长期V79细胞DNA聚合酶β

Keywords

RadiationCMPlateau phase V79 cellsDNApolymerase β

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