CHANGES OF DNA POLYMERASE β GENE TRANSCRIPTION AND EXPRESSION IN HEPATOMA AFTER IRRADIATION

Cai Jianming; Zheng Xiulong; Gao Jianguo; Yu Hongyu; Li Bailong; Dong Junrui; Luo Chengji

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CHANGES OF DNA POLYMERASE β GENE TRANSCRIPTION AND EXPRESSION IN HEPATOMA AFTER IRRADIATION

更新时间：2023-04-23

- CHANGES OF DNA POLYMERASE β GENE TRANSCRIPTION AND EXPRESSION IN HEPATOMA AFTER IRRADIATION
- Journal of Radiation Research and Radiation Processing Vol. 15, Issue 1, Pages: 44-47(1997)
- 作者机构：
  
  1.第二军医大学放射医学研究所　上海　200433
  2.第三军医大学防原教研室　重庆　630038
- 作者简介：
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Cai Jianming, Zheng Xiulong, Gao Jianguo, et al. CHANGES OF DNA POLYMERASE β GENE TRANSCRIPTION AND EXPRESSION IN HEPATOMA AFTER IRRADIATION. [J]. Journal of Radiation Research and Radiation Processing 15(1):44-47(1997)
DOI：

Cai Jianming, Zheng Xiulong, Gao Jianguo, et al. CHANGES OF DNA POLYMERASE β GENE TRANSCRIPTION AND EXPRESSION IN HEPATOMA AFTER IRRADIATION. [J]. Journal of Radiation Research and Radiation Processing 15(1):44-47(1997) DOI：

摘要

采用免疫组织化学和胞浆斑点杂交法，研究了γ射线照射后SMMC-LTNM肝癌细胞DNA聚合酶,β,基因转录和表达的变化。结果显示，肝癌细胞在体照射2 Gy、4 Gy后24～48 h，细胞内该酶基因表达增强，酶含量增加，照射后72 h基本恢复至照射前状况；该酶基因转录也出现增强，其mRNA含量有一过性升高，照射后36～48 h达峰值，约为照射前水平的1.76倍。这些结果提示，γ射线照射后肝癌细胞内DNA聚合酶,β,的作用得到加强，可能对肿瘤的放疗产生不利影响。

Abstract

Upon the previous studies about the effect of radiation on the DNA polymerase β (pol β) activity and the roles of the enzyme on DNA repair synthesis, we reported the effects of γ-irradiation on gene transcription and its expression of DNA pol β. After 2 or 4 Gy total body irradiation, some biological properties of the enzyme in SMMC-LTNM hepatoma cells borne in nude mice changed. The content of DNA pol β mRNA in hepatoma cells increased significantly, reached the maximum (about 1.76-fold of control) at 36 hours, then decreased closely to control level 3 days later. The gene expression tested by immuno-gold-silver staining assay exhibited some alterations which showed that the enzyme gene expression become more active at 48 hours after irradiation. These results indicated that γ-irradiation would promote DNA pol β transcription and enhanced its DNA repair function.

关键词

DNA聚合酶β基因转录基因表达γ射线SMMC-LTNM肝癌

Keywords

DNA polymerase βGene transcriptionGene expressionGammaraysSMMC-LTNM hepatoma

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