Assessment of the protective effects of GANRA nanoparticles against X-ray radiation-induced cellular damage and microRNA alterations

Sulan LIN; Nan DING; Wenjun WEI; Jinpeng HE; Jufang WANG

doi:10.118879/j.1000-3436.2017.rrj.35.030201

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Assessment of the protective effects of GANRA nanoparticles against X-ray radiation-induced cellular damage and microRNA alterations

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- Assessment of the protective effects of GANRA nanoparticles against X-ray radiation-induced cellular damage and microRNA alterations
- Journal of Radiation Research and Radiation Processing Vol. 35, Issue 3, (2017)
- 作者机构：
  
  1.中国科学院近代物理研究所甘肃省空间辐射生物学重点实验室兰州 730000
  2.中国科学院大学北京 100049
- 作者简介：
- 基金信息：
  
  National Science Foundation(31270895);Special national international technology cooperation(2015DFR30940);Manned space program in advance(040101)
- DOI：10.118879/j.1000-3436.2017.rrj.35.030201
  CLC：
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Sulan LIN, Nan DING, Wenjun WEI, et al. Assessment of the protective effects of GANRA nanoparticles against X-ray radiation-induced cellular damage and microRNA alterations. [J]. Journal of Radiation Research and Radiation Processing 35(3):030201-8(2017)
DOI：

Sulan LIN, Nan DING, Wenjun WEI, et al. Assessment of the protective effects of GANRA nanoparticles against X-ray radiation-induced cellular damage and microRNA alterations. [J]. Journal of Radiation Research and Radiation Processing 35(3):030201-8(2017) DOI： 10.118879/j.1000-3436.2017.rrj.35.030201.

摘要

以淋巴母细胞（PENG-EBV）作为细胞模型，采用细胞增殖率检测、微核率检测以及胞内活性氧（Reactive oxygen species，ROS）含量测定等方法检测GANRA纳米药对淋巴母细胞的毒性作用和辐射防护效果。同时，检测药物对淋巴母细胞中几种辐射敏感microRNA（miRNA）辐射的影响。实验结果表明：GANRA纳米药对淋巴母细胞没有毒性作用，且加药后对细胞增殖有促进作用；GANRA纳米药预处理可以缓解淋巴母细胞由X-射线产生的辐射损伤，提高辐照后细胞活性、细胞存活率以及降低细胞的微核形成率，并且对X-射线辐照产生的自由基具有良好的清除作用。此外，miR-150、miR-57、miR-223和miR-34a对GANRA纳米药具有敏感性，GANRA纳米药可能通过影响辐射敏感miRNA的表达发挥辐射防护作用。结果提示，改进后的GANRA纳米药物可以作为一种更有优势的辐射防护药物，并且给药后miRNA的差异性表达可能是GANRA纳米药物发挥作用的一种新机制。

Abstract

The radioprotective effects of GANRA nanoparticles against X-ray radiation were studied in lymphoblast cells. Cytotoxicity and cell proliferation assays were conducted to evaluate the toxicity and radioprotective effects of the nanoparticles. The influence of these nanoparticles on the induction of genomic instability was determined using the cytokinesis-block micronucleus assay. Our results indicated that GANRA nanoparticles exhibited low toxicity, while protecting lymphoblasts from X-ray radiation-induced damage. Moreover, GANRA nanoparticles acted as free radical scavengers. GANRA nanoparticles could also alter the sensitivity of circulating microRNAs to radiation, as assessed by the relative microRNA expression in lymphoblasts. In conclusion, our results suggest that GANRA nanoparticles have potential use as safe and efficient radioprotectants.

关键词

GANRA纳米药X-射线microRNA/miRNA淋巴母细胞

Keywords

GANRA nanoparticlesX-raysmicroRNA/miRNALymphoblast cells

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苏州大学医学部放射医学与防护学院

School of Radiation Medicine and Protection, Medical School of Soochow University

1（兰州大学药学院兰州 730000）2（中国科学院近代物理研究所）

3（甘肃省微生物资源开发利用重点实验室）

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