Irradiation protection effects of magnesium sulfate on human umbilical vein endothelial cells

Yang SUN; Yu TU

doi:10.11889/j.1000-3436.2016.rrj.34.020201

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Irradiation protection effects of magnesium sulfate on human umbilical vein endothelial cells

更新时间：2021-12-10

- Irradiation protection effects of magnesium sulfate on human umbilical vein endothelial cells
- Journal of Radiation Research and Radiation Processing Vol. 34, Issue 2, Pages: 20201(2016)
- 作者机构：
  
  1.苏州大学医学部放射医学与防护学院苏州 215123
  2.School of Radiation Medicine and Protection, Medical School of Soochow University, Suzhou 215123, China
- 作者简介：
- 基金信息：
- DOI：10.11889/j.1000-3436.2016.rrj.34.020201
  CLC：
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Yang SUN, Yu TU. Irradiation protection effects of magnesium sulfate on human umbilical vein endothelial cells. [J]. Journal of Radiation Research and Radiation Processing 34(2):20201(2016)
DOI：

Yang SUN, Yu TU. Irradiation protection effects of magnesium sulfate on human umbilical vein endothelial cells. [J]. Journal of Radiation Research and Radiation Processing 34(2):20201(2016) DOI： 10.11889/j.1000-3436.2016.rrj.34.020201.

摘要

对数生长期的人脐静脉血管内皮细胞(Human umbilical vein endothelial cells, HUVECs) 分3组进行不同处理后采用流式细胞仪检测细胞周期分布;Annexin V/PI双染法测定细胞凋亡率;Western blot检测Bcl-2蛋白的表达情况;RT-PCR法检测Bcl-2 mRNA的表达情况。结果显示,硫酸镁可以在X-射线照射24 h后改善HUVECs细胞G2/M期阻滞;在辐照后24、48、72 h可以提高细胞对X-射线的辐射敏感性,并增加细胞凋亡率(,t,=4.24、7.19、3.20,p,<,0.05);在辐照后48、72 h能明显抑制抗凋亡蛋白Bcl-2蛋白的表达;照后24、48、72 h 的Bcl-2 mRNA表达量减少(,t,=3.034、6.182、3.957,p,<,0.05)。结果提示,硫酸镁可以缓解X-射线照射后细胞G2/M期阻滞,增加照射后HUVECs的细胞凋亡率,降低X-射线诱导的抗凋亡蛋白Bcl-2的表达。

Abstract

The human umbilical vein endothelial cells (HUVECs) of logarithmic growth phase were divided into three groups for different treatment. And then they were detected with the cell cycle distribution by flow cytometry, rate of apoptosis by annexin V/PI double staining method, expression of Bcl-2 protein by Western blot and expression of Bcl-2 mRNA by RT-PCR. The results showed that magnesium sulfate could advance the G2/M phase block of HUVECs after X-ray irradiation for 24 h, and improve the sensitivity of HUVECs to X-rays after irradiation for 24 h, 48 h and 72 h, increasing apoptosis rate to a certain extent (,t,=4.24, 7.19, 3.20,p,<,0.05) and decreasing Bcl-2 mRNA expression (,t,=3.034, 6.182, 3.957,p,<,0.05)after irradiation for 24 h, 48 h and 72 h, as well as inhibit the anti-apoptotic protein Bcl-2 protein expression significantly after irradiation for 48 h and 72 h. Therefore, magnesium sulfate could improve the G2/M phase arrest, increase the apoptosis rate and reduce Bcl-2 expression of HUVECs irradiated with X-rays.

关键词

硫酸镁人脐静脉血管内皮细胞X-射线细胞凋亡Bcl-2

Keywords

Magnesium sulfateHuman umbilical vein endothelial cellsX-raysCell apoptosisBcl-2

references

刘强 . 放射性脑损伤研究现状 . 国外医学: 放射医学核医学分册 , 2004 . 28 (4 ):178 -181. .

LIU Qiang . The study status of radiation induced brain injury . Foreign Medical Sciences: Section of Radiation Medicine and Nuclear Medicine , 2004 . 28 (4 ):178 -181. .

李雨民 . DNA损伤修复与细胞凋亡 . 国外医学: 放射医学核医学分册 , 1998 . 23 (3 ):112 -115. .

LI Yumin . DNA damage repair and cell apoptosis . Foreign Medical Sciences: Section of Radiation Medicine and Nuclear Medicine , 1998 . 23 (3 ):112 -115. .

封士成 , 崔凤梅 , 涂彧 . 硫酸镁对人脐静脉血管内皮细胞辐射防护的作用 . 辐射研究与辐射工艺学报 , 2014 . 32 (2 ):020204 .

FENG Shicheng , CUI Fengmei , TU Yu . Magnesium sulfate on human umbilical vein endothelial cells in radiation protection effect . Journal of Radiation Research and Radiation Processing , 2014 . 32 (2 ):020204 .

刘萍 , 涂彧 . 硫酸镁对大鼠神经干细胞辐射损伤保护作用的初步研究 . 中华放射医学与防护杂志 , 2010 . 30 (6 ):639 -642. .

LIU Ping , TU Yu . Magnesium sulfate on rat neural stem cell radiation injury: a preliminary study . Chinese Journal of Radiological Medicine and Protection , 2010 . 30 (6 ):639 -642. .

郑慧芬 , 涂彧 . 硫酸镁对急性放射性脑损伤后神经细胞凋亡的影响 . 现代中西医结合杂志 , 2009 . 18 (4 ):364 -367. .

ZHENG Huifen , TU Yu . Effect of magnesium sulfate induced the nerve cell apoptosis combined with radiation after acute brain injury . Modern Journal of Integrated Traditional Chinese and Western Medicine , 2009 . 18 (4 ):364 -367. .

徐华林 , 孙阳 , 崔凤梅 , 等 . 硫酸镁抑制经辐射后人脐静脉血管内皮细胞γ-H2AX的表达 . 中华放射医学与防护杂志 , 2015 . 35 (3 ):161 -164. .

XU Hualin , SUN Yang , CUI Fengmei , 等 . Magnesium sulfate inhibited the expression of γ-H2AX on human umbilical vein endothelial cells after irradiation . Chinese Journal of Radiological Medicine and Protection , 2015 . 35 (3 ):161 -164. .

Yuan H , Goets D J , Gaber M W , 等 . Radiation-induced permeability and leukocyte adhesion in the rat blood-brain barrier: modulation with anti-ICAM-1 antibody . Brain Research , 2003 . 969 (1-2 ):59 -69. .

马辰莺 , 徐晓婷 , 涂彧 , 等 . VEGF mRNA及蛋白在大鼠放射性脑损伤模型中的动态变化 . 中华放射医学与防护杂志 , 2014 . 34 (6 ):405 -410. .

MA Chenying , XU Xiaoting , TU Yu , 等 . The dynamic changes of VEGF mRNA and protein in rat model of radiation brain injury . Chinese Journal of Radiation Medicine and Protection , 2014 . 34 (6 ):405 -410. .

Fokas E , Kraft G , An H , 等 . Ion beam radiobiology and cancer: time to update ourselves . Acta Biochimica et Biophysica Sinica , 2009 . 1796 (2 ):216 -229. .

Wang B F , Dai Z J , Wang X J , 等 . Saikosaponin-dincreases the radiosensitivity of smmc-7721 hepatocellular carcinoma cells cycle . BMC Comple-mentary and Alternative Medicine , 2013 . 13 (1 ):263 .

Yin Y L , Luciani M G , Fahraeus R . P53 stability and activity is regulated by mdm2-mediated induction of alternative p53 translation products . Nature Cell Biology , 2002 . 4 462 -467. .

钱鑫 , 朱应葆 . DNA放射损伤与P53 . 生理科学进展 , 2005 . 36 (4 ):379 -381. .

QIAN Xin , ZHU Yingbao . Progress in DNA radiation injury and P53 . Progress in Physiological Sciences , 2005 . 36 (4 ):379 -381. .

Trieb K , Eberl T , Steger M , 等 . Apoptosis is involved in endothelial cell damage during preservation and influenced by organ storage solutions . Transplantation Proceedings , 1997 . 29 (1-2 ):416 -418. .

Sorrentino G , Mioni M , Giorgi C , 等 . The prolyl-isomerase Pin1 activates the mitochondrial death program of p53 . Cell Death and Differentiation , 2013 . 20 (2 ):198 -208. .

李莉 , 古正涛 , 刘志峰 , 等 . 活性氧调控Bcl-2、Bax表达参与热打击后人脐静脉内皮细胞凋亡的研究 . 中华危重病急救医学 , 2014 . 26 (7 ):458 -463. .

LI Li , GU Zhengtao , LIU Zhifeng , 等 . The effect of reactive axygen species regulation of expression Bcl-2 and Bax in apoptosis of human umbilical vein endothelial cell induced by heat stress . Chinese Critical Care Medicine , 2014 . 26 (7 ):458 -463. .

Lalier L , Cartron P F , Juin P , 等 . Bax activation and mitochondrial insertion during apoptosis . Apoptosis , 2007 . 12 (5 ):887 -896. .

任妙丹 , 何爱文 , 陈寿权 , 等 . 心肺复苏后大鼠皮质区凋亡相关性微小RNA的表达变化 . 中国中西医结合急救杂志 , 2014 . 21 (2 ):95 -98. .

REN Miaodan , HE Aiwen , CHEN Shouquan , 等 . The expression changes of small RNA in the cortical areas of rats after cardiopulmonary resuscitation and the expression change . Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care , 2014 . 21 (2 ):95 -98. .

张丽群 , 符州 . Bcl-2、Bax与气道上皮细胞凋亡的研究进展 . 检验医学与临床 , 2007 . 4 (4 ):285 -287. .

ZHANG Liqun , FU Zhou . Research progress of Bcl-2, Bax and apoptosis of airway epithelial cells . Laboratory Medicine and Clinic , 2007 . 4 (4 ):285 -287. .

Aradhya S , Nelson D L . NF-kappa B signaling and human disease . Current Opinion in Genetics & Development , 2001 . 11 (3 ):300 -306. .

周桂凤 , 蒋云生 , 胡白英 , 等 . 铅抑制NF-κB、Bcl-2蛋白表达诱导人肾小管内皮细胞凋亡 . 实用预防医学 , 2008 . 15 (5 ):1330 -1332. .

ZHOU Guifeng , JIANG Yunsheng , HU Baiying , 等 . Effect of lead on apoptosis and expression of NF-Kb, Bcl-2 in human renal tubular endothelial cell line HK-2 . Practical Preventive Medicine , 2008 . 15 (5 ):1330 -1332. .

Wang C Y , Mayo M W , Korneluk R G , 等 . NF-kB antiapoptosis: induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to suppress caspase-8 activation . Science , 1998 . 281 (5383 ):1680 -1683. .

Starenki D V , Hiroyuki N , Saenko V A , 等 . Induction of thyroid cancer cell apoptosis by a novel NF-kB inhibitor, dehydruoymethylepoxyquinomicin . Clinical Cancer Research , 2004 . 10 (20 ):6821 -6829. .

Catz S D , Johnson J L . Transcriptional regulation of Bcl-2 by nuclear factor kappa B and its significance in prostate cancer . Oncogene , 2001 . 20 (50 ):7342 -7. .

Viatour P , Bentiers-Alj M , Chariot A , 等 . NF-kappa B2/p100 induces Bcl-2 expression . Leukemia , 2003 . 17 (7 ):1349 -1356. .

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