Protective effects of the plant lectin PHA-L on acute radiation injuries in mice

Guanghui ZHANG; Hongying WU; Jin JIN; Zewei ZHOU; Peixun LIU; Wei LONG

doi:10.11889/j.1000-3436.2016.rrj.34.020203

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Protective effects of the plant lectin PHA-L on acute radiation injuries in mice

更新时间：2021-12-10

- Protective effects of the plant lectin PHA-L on acute radiation injuries in mice
- Journal of Radiation Research and Radiation Processing Vol. 34, Issue 2, Pages: 20203(2016)
- 作者机构：
  
  1.中国医学科学院北京协和医学院放射医学研究所天津 300192
  2.Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China
- 作者简介：
- 基金信息：
- DOI：10.11889/j.1000-3436.2016.rrj.34.020203
  CLC：
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Guanghui ZHANG, Hongying WU, Jin JIN, et al. Protective effects of the plant lectin PHA-L on acute radiation injuries in mice. [J]. Journal of Radiation Research and Radiation Processing 34(2):20203(2016)
DOI：

Guanghui ZHANG, Hongying WU, Jin JIN, et al. Protective effects of the plant lectin PHA-L on acute radiation injuries in mice. [J]. Journal of Radiation Research and Radiation Processing 34(2):20203(2016) DOI： 10.11889/j.1000-3436.2016.rrj.34.020203.

摘要

观察植物凝集素PHA-L对小鼠急性辐射损伤的保护作用及相关机制。采用动物30 d存活率实验,即小鼠全身辐照至吸收剂量为7.2 Gy后观察其存活情况;利用外周血和免疫学实验,小鼠全身照射至吸收剂量为7.2 Gy后测外周血白细胞数(White blood cell, WBC)、股骨有核细胞数(The number of nucleated cells in bone marrow, BMNC)、骨髓DNA含量以及各脏器指数;对小鼠进行9.0 Gy腹部照射,取小肠组织做病理切片观察。结果显示,照射给药组小鼠30 d存活率比单纯照射组均有提高,特别是高剂量组提高约60%;与单纯照射组相比,照射给药高剂量组的白细胞数、骨髓DNA含量和脾结节数均有提高,分别从0.74±0.16、1.02±0.17和9.80±6.46提高到1.18±0.40、1.22±0.17和18.10±6.87,数据均有统计学意义(,p,<,0.05),并且PHA-L对脏器也有一定的保护作用;与单纯照射组相比,照射给药高剂量组小鼠肠道组织损伤恢复明显。提示植物凝集素PHA-L对急性辐射损伤具有保护作用,但其作用机制有待进一步研究。

Abstract

The aim is to observe the protective effects of plant lectin PHA-L against radiation injuries and the relative mechanism. 30 d survival experiment was used to observe the survival condition of mice 30 d after irradiation with 7.2 Gy γ-rays. Blood and immunological experiments of mice were carried out to observe WBC (White blood cell), femoral nucleated cells, bone marrow DNA content and organs index after irradiation with 7.2 Gy γ-rays. Intestine pathological section of mice irradiated on abdomen with 9.0 Gy γ-rays was prepared to observe the pathology of intestine. The results showed that compared with the irradiation group, the survival rate of mice treated with PHA-L increased, especially for the high dose group (60% lifted); compared with the irradiation group, white blood cell count, bone marrow DNA content and spleen nodules of mice administered with high dose group increased from 0.74±0.16, 1.02±0.17 and 9.80±6.46 to 1.18±0.40, 1.22±0.17 and 18.10±6.87 respectively, which were statistically significant (,p,<,0.05). Moreover, PHA-L also had some protective effect on the organ. In pathology observation, the intestines of mice treated with PHA-L recovered significantly as well. Therefore, plant lectin PHA-L can protect mice from radiation injuries, yet the mechanism needs further study.

关键词

辐射损伤植物凝集素PHA-L放射性肠炎

Keywords

Radiation damagePlant lectin PHA-LRadiation intestine

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