Radiation protection effects of Vam3 on mouse thymocyte <italic style="font-style: italic">in vitro</italic>

Bing LIU; Junling ZHANG; Deguan LI; Jin PAN; Yueying WANG; Chunsuo YAO; Qi HOU; Aimin MENG

doi:10.11889/j.1000-3436.2016.rrj.34.020207

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Radiation protection effects of Vam3 on mouse thymocyte in vitro

更新时间：2021-12-10

- Radiation protection effects of Vam3 on mouse thymocyte in vitro
- Journal of Radiation Research and Radiation Processing Vol. 34, Issue 2, Pages: 20207(2016)
- 作者机构：
  
  1.北京协和医学院中国医学科学院医学实验动物研究所北京 100021；
  2.北京协和医学院中国医学科学院放射医学研究所天津 300192；
  3.北京协和医学院中国医学科学院药物研究所北京 100050
- 作者简介：
- 基金信息：
- DOI：10.11889/j.1000-3436.2016.rrj.34.020207
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Bing LIU, Junling ZHANG, Deguan LI, et al. Radiation protection effects of Vam3 on mouse thymocyte in vitro. [J]. Journal of Radiation Research and Radiation Processing 34(2):20207(2016)
DOI：

Bing LIU, Junling ZHANG, Deguan LI, et al. Radiation protection effects of Vam3 on mouse thymocyte in vitro. [J]. Journal of Radiation Research and Radiation Processing 34(2):20207(2016) DOI： 10.11889/j.1000-3436.2016.rrj.34.020207.

摘要

探讨白藜芦醇二聚体(Amurensis H, Vam3)对辐射致小鼠胸腺细胞损伤的保护作用。体外无菌分离小鼠胸腺细胞,实验分为6组:对照组(Control)、白藜芦醇组(Resveratrol, Res)、Vam3组、照射组(Irradiation, IR)、照射+ Res组和照射+Vam3组。照射+Res组和照射+Vam3组于照射前30 min给予Vam3和Res孵育,对照组、Res组、Vam3组以及照射组加等量RPMI-1640培养基或对应药物处理,除对照组、Res组和Vam3组外,其余3组均给予4 Gy ,137,Cs γ-射线单次照射。照射后分别用生物发光法检测小鼠胸腺细胞活力,活性氧探针(DCFH-DA)检测细胞内活性氧(Reactive oxygen species, ROS)水平,荧光抗体标记检测γ-H2AX表达水平以及FITC-Annexin V和PI双染标记法检测细胞早期凋亡率。结果显示,与对照组比较,照射组细胞活力显著下降,细胞内ROS和γ-H2AX水平明显升高,同时细胞早期凋亡数目增加;而照射+Vam3组与照射+Res组比较显示,Vam3在提升细胞活力,降低细胞内ROS和γ-H2AX水平以及抑制细胞凋亡方面的作用比Res更加明显。研究结果表明,Vam3对辐射引起的小鼠胸腺细胞急性损伤有一定保护作用,且保护作用优于白藜芦醇。

Abstract

The aim is to observe the protective effect of Vam3 on irradiation-induced injury of thymocytes isolated sterilely from mice. The thymocytes were divided into 6 groups: control group, resveratrol group, Vam3 group, irradiation group, irradiation with resveratrol group and irradiation with Vam3 group. Except the control group, resveratrol group and Vam3 group, the rest groups were exposed to radiation at a single dose of 4 Gy γ-rays from ,137,Cs after 30 min treatment by vehicle and tested drug, respectively. The thymocytes' viability was measured by bioluminescence; the level of thymocytes' reactive oxygen species (ROS) was measured by DCFH-DA; the level of thymocytes' γ-H2AX was measured by fluorescent antibody labeling; the early apoptosis was detected by FITC-Annexin V and PI labelling using flow cytometry (FCM) after radiation. Compared with control group, the thymocytes' cell viability decreased obviously after irradiation, and the level of cell ROS, γ-H2AX and early apoptosis cell increased. By comparing the resveratrol group and Vam3 group with the irradiation group, we can find that Res and Vam3 could reduce the decrease of thymocytes' cell viability and inhibit the increase of ROS, γ-H2AX and early apoptosis cell induced by irradiation. The comparison between irradiation with resveratrol group and irradiation with Vam3 group showed that the effects of Vam3 in increasing cell viability, decreasing the level of ROS and γ-H2AX and lowering the early apoptosis rate were more obvious than resveratrol. It can be concluded that Vam3 performs better than resveratrol in the protection of mice thymocytes against the acute radiation injury.

关键词

白藜芦醇二聚体白藜芦醇胸腺细胞辐射损伤辐射防护剂

Keywords

Vam3ResveratrolThymocyteRadiation injuriesRadioprotector

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Department of Biopharmaceutics, School of Pharmacy, The Fourth Military Medical University

Clinical Laboratory, 401 General Military Hospital

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College

National Institute for Radiological Protection, Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College & Chinese Academy of Medical Science

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Radiation protection effects of Vam3 on mouse thymocyte in vitro

DOI：10.11889/j.1000-3436.2016.rrj.34.020207

摘要

Abstract

关键词

Keywords

references