Study on the actin assembly on different substrates

Xueqiang ZHANG; Xiuyuan HU; Haozhi LEI; Ying WANG; Jun HU; Yi ZHANG

doi:10.11889/j.1000-3436.2016.rrj.34.040701

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Study on the actin assembly on different substrates

RADIATION INTERDISCIPLINARY RESEARCH | 更新时间：2021-12-10

- Study on the actin assembly on different substrates
- Journal of Radiation Research and Radiation Processing Vol. 34, Issue 4, Pages: 51-56(2016)
- 作者机构：
  
  1.中国科学院上海应用物理研究所嘉定园区上海 201800
  2.中国科学院大学北京 100049
- 作者简介：
- 基金信息：
  
  the National Basic Research Program of China(2013CB932801);the National Natural Science Foundation ofChina(11274334);the Chinese Academy of Sciences(KJZD-EW-M03)
- DOI：10.11889/j.1000-3436.2016.rrj.34.040701
  CLC：
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Xueqiang ZHANG, Xiuyuan HU, Haozhi LEI, et al. Study on the actin assembly on different substrates. [J]. Journal of Radiation Research and Radiation Processing 34(4):51-56(2016)
DOI：

Xueqiang ZHANG, Xiuyuan HU, Haozhi LEI, et al. Study on the actin assembly on different substrates. [J]. Journal of Radiation Research and Radiation Processing 34(4):51-56(2016) DOI： 10.11889/j.1000-3436.2016.rrj.34.040701.

摘要

采用兔骨骼肌Actin作为研究对象，通过搭建原子力显微镜（Atomic force microscope，AFM）液相实时进样装置观察并对比G-actin在不同衬底上的组装过程。实验中发现，Actin在云母衬底上组装时形成较少的成核位点，然后组装成长达几十微米的纤维；而在带有正电荷的磷脂膜（DPPC：DPTAP=4：1）衬底上则快速大量地成核，组装形成较短的纤维。成像时所用的机械力应≤50 pN，所用的衬底应带有一定的正电荷。研究结果为构建Actin轨道上的纳米分子马达运输系统提供了依据。

Abstract

The assembly processes of actin on mica substrate and lipid membrane by real time imaging of atomic force microscope (AFM) and laser scanning confocal microscope were studied. It was found that on the bare mica substrate, the small amount of G-actin nucleating points formed and subsequently self-assembled into long actin filaments with lengths up to tens of micro-meters. While on the positively-charged lipid membrane (DPPC:DPTAP=4:1), large amounts of nucleating points quickly formed, leading to the formation of relatively shorter filaments. The proposed optimum condition for exploring actin assembly process with AFM ,in vitro, is positively-charged substrates should be used while the AFM imaging force should be lower than 50 pN. These results laid foundation for construction of micro- and nano-scale transport systems based on actin.

关键词

原子力显微镜肌动蛋白自组装分子马达

Keywords

Atomic force microscope (AFM)ActinSelf-assemblyMolecular motor

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