Cloning, and functional analysis of <italic style="font-style: italic">DR_2327</italic> and <italic style="font-style: italic">DR_2328</italic> in Deinococcus radiodurans

Yuhong YANG

doi:10.11889/j.1000-3436.2016.rrj.34.050203

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Cloning, and functional analysis of DR_2327 and DR_2328 in Deinococcus radiodurans

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- Cloning, and functional analysis of DR_2327 and DR_2328 in Deinococcus radiodurans
- Journal of Radiation Research and Radiation Processing Vol. 34, Issue 5, (2016)
- 作者机构：
  
  1.永州职业技术学院永州 425100
- 作者简介：
- 基金信息：
- DOI：10.11889/j.1000-3436.2016.rrj.34.050203
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Yuhong YANG. Cloning, and functional analysis of DR_2327 and DR_2328 in Deinococcus radiodurans. [J]. Journal of Radiation Research and Radiation Processing 34(5):050203-8(2016)
DOI：

Yuhong YANG. Cloning, and functional analysis of DR_2327 and DR_2328 in Deinococcus radiodurans. [J]. Journal of Radiation Research and Radiation Processing 34(5):050203-8(2016) DOI： 10.11889/j.1000-3436.2016.rrj.34.050203.

摘要

通过生物信息学分析耐辐射奇球菌,DR_2327,和,DR_2328,基因及编码蛋白的基本性质，利用PCR方法克隆,DR_2327,和,DR_2328,的全基因，连接至pGEM-T载体上，转化至大肠杆菌JM109中，并进行双酶切鉴定。生物信息学分析结果表明，,DR_2327,和,DR_2328,基因位于同一操纵子中，且DR_2328蛋白具有组氨酸激酶活性，DR_2327蛋白具有反应调节子功能，提示两者很可能组成一对双组分系统。体外实验表明，,DR_2327,和,DR_2328,基因的外源表达在一定程度上提高了大肠杆菌对紫外辐照和H,2,O,2,的耐受能力。

Abstract

The main characteristics of ,DR_2327,and ,DR_2328, in Deinococcus radiodurans were analyzeed by bioinformatics. Full-length gene of ,DR_2327, and ,DR_2328, was amplified by PCR, and constructed in pGEM-T vector. Transformed recombinant vector into E.coli JM109 and followed by restriction enzyme digestion. ,DR_2327, and ,DR_2328, gene was cloned successfully. Bioinformatic analysis results showed that ,DR_2327, and ,DR_2328, gene located in the same operon, and DR_2328 protein with histidine kinase activity, DR_2327 protein with response regulator activity. It is suggested that ,DR_2327, and ,DR_2328, constitute a two-component system in Deinococcus radiodurans. ,In vitro, experiments showed that ,DR_2327, and ,DR_2328, could enhance tolerance to UV radiation and H,2,O,2, of ,E.coli,.

关键词

耐辐射奇球菌DR_2327DR_2328基因克隆生物信息学分析

Keywords

Deinococcus radioduransDR_2327DR_2328Gene cloningBioinformatics analysis

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School of Public Health, Hengyang Medical College University of South China

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1 (School of Public Health, University of South China,,)

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Cloning, and functional analysis of DR_2327 and DR_2328 in Deinococcus radiodurans

DOI：10.11889/j.1000-3436.2016.rrj.34.050203

摘要

Abstract

关键词

Keywords

references