Expression of FOXM1 and its effects on radiation sensitivity in esophageal squamous cell carcinoma (ESCC)

Langhuan LIU; Aiwu LUO; Keliang CHEN; Mengmeng GU; Beibei XIAO; Shitao ZOU; Chao HE; Jundong ZHOU; Bo HUANG

doi:10.11889/j.1000-3436.2016.rrj.34.060202

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Expression of FOXM1 and its effects on radiation sensitivity in esophageal squamous cell carcinoma (ESCC)

Radiobiology and Radiomedicine | 更新时间：2021-12-10

- Expression of FOXM1 and its effects on radiation sensitivity in esophageal squamous cell carcinoma (ESCC)
- Journal of Radiation Research and Radiation Processing Vol. 34, Issue 6, (2016)
- 作者机构：
  
  1.南华大学公共卫生学院衡阳 421001
  2.苏州大学放射医学与防护学院苏州 215123
  3.南京医科大学附属苏州市立医院南京 215001
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of Hunan Province(2016JJ2115);Doctor stand-up foundation of University of South China(2015XQD31);Health and Family Planning Commission Scientific Research of Hunan Province(C2015-17)
- DOI：10.11889/j.1000-3436.2016.rrj.34.060202
  CLC：
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Langhuan LIU, Aiwu LUO, Keliang CHEN, et al. Expression of FOXM1 and its effects on radiation sensitivity in esophageal squamous cell carcinoma (ESCC). [J]. Journal of Radiation Research and Radiation Processing 34(6):060202-8(2016)
DOI：

Langhuan LIU, Aiwu LUO, Keliang CHEN, et al. Expression of FOXM1 and its effects on radiation sensitivity in esophageal squamous cell carcinoma (ESCC). [J]. Journal of Radiation Research and Radiation Processing 34(6):060202-8(2016) DOI： 10.11889/j.1000-3436.2016.rrj.34.060202.

摘要

采用Real-time PCR检测40例食管鳞状细胞癌及相对应癌旁组织中FOXM1（Forkhead box M1）mRNA表达情况；免疫组织化学方法检测94例食管鳞状细胞癌组织中FOXM1蛋白的表达情况，分析其与鳞状细胞癌临床病理之间的关系；采用针对FOXM1的siRNA沉默ECA-109、TE-1细胞中FOXM1基因表达水平，通过免疫荧光染色、克隆形成实验检测FOXM1对食管鳞状细胞癌放射敏感性的影响。结果表明，FOXM1 mRNA表达水平在食管癌组织中显著高于癌旁组织（,p,<, 0.01），其蛋白表达水平与病人生存期负相关。siRNA能有效干扰ECA-109细胞中的FOXM1表达水平，FOXM1沉默细胞中X-射线诱发的,γ,H2AX焦点显著多于对照细胞，FOXM1沉默细胞电离辐射后克隆形成能力显著低于对照细胞。结果提示，FOXM1在食管鳞癌中的表达增加，其表达水平与病人预后负相关；FOXM1基因沉默可显著增强细胞放射敏感性，因此，FOXM1有可能成为食管鳞癌的治疗靶点。

Abstract

Real-time PCR was used to examine the expression of FOXM1 (Forkhead box M1) in 40 esophageal squamous cell carcinoma (ESCC) tissues, and the adjacent normal tissues. Immunohistochemistry was used to examine the expression of FOXM1 protein in 94 ESCC tissues, and to analyze the relationship between ESCC and clinic pathologic. A small interfering RNA targeting FOXM1 was designed to silence the endogenous FOXM1 expression of ECA-109 and TE-1 cells. The effect of FOXM1 on radiation sensitive in esophageal squamous cell was observed by immunofluorescence stain, and colony formation assay. The results showed that FOXM1 mRNA expression level in esophageal cancer tissues was significantly higher than that of the adjacent tissues (,p,<, 0.01), and the protein level expression of it has negative correlation with the survival times; the designed siRNA could dramatically silence FOXM1 expression in ECA-109 cells; ,γ,H2AX focus induced by X-rays in FOXM1 silent cells was significantly higher than that in control cells; the clone formation ability of the silent FOXM1 group was significantly lower than that in the control group. The content of FOXM1 is improved in ESCC, and is negatively correlated with the prognosis of patients; the cell radiation sensitive proliferation is significantly improved in the silent FOXM1 ECA-109 cells. In conclusion, FOXM1 has the potential to be the treatment of esophageal squamous carcinoma target.

关键词

食管鳞癌FOXM1不良预后辐射敏感性DNA损伤修复

Keywords

Esophageal squamous cell carcinomaFOXM1 poor prognosisRadiation sensitivityDNA damage repair

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