Effect of wide-band electromagnetic pulses on the reproductive system of rat and subsequent recovery

He LIU; Mingjia LEI; Jie ZHANG; Hongxing QI; Xianghui WANG

doi:10.11889/j.1000-3436.2017.rrj.35.010203

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Effect of wide-band electromagnetic pulses on the reproductive system of rat and subsequent recovery

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- Effect of wide-band electromagnetic pulses on the reproductive system of rat and subsequent recovery
- Journal of Radiation Research and Radiation Processing Vol. 35, Issue 1, Pages: 10203(2017)
- 作者机构：
  
  1.华东师范大学物理与材料科学学院生物物理研究所上海 200241
- 作者简介：
- 基金信息：
  
  National Basic Research Program of China(2011CB503701);National Natural Science Foundation of China(31600675)
- DOI：10.11889/j.1000-3436.2017.rrj.35.010203
  CLC：
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He LIU, Mingjia LEI, Jie ZHANG, et al. Effect of wide-band electromagnetic pulses on the reproductive system of rat and subsequent recovery. [J]. Journal of Radiation Research and Radiation Processing 35(1):10203(2017)
DOI：

He LIU, Mingjia LEI, Jie ZHANG, et al. Effect of wide-band electromagnetic pulses on the reproductive system of rat and subsequent recovery. [J]. Journal of Radiation Research and Radiation Processing 35(1):10203(2017) DOI： 10.11889/j.1000-3436.2017.rrj.35.010203.

摘要

采用酶联免疫吸附法测定血清睾酮、精子顶体酶、睾丸组织中超氧化物歧化酶和丙二醛含量；CASA系统检测精子的活力指标；TUNEL法检测睾丸组织中生精细胞的凋亡情况。结果表明，大鼠生殖系统所受到的即时损伤与修复与辐照脉冲的场强和脉冲数有关，场强越高，脉冲数越大，损伤越明显，修复越困难。结果提示，在实验条件下，场强的影响比脉冲次数的影响更显著。

Abstract

The testosterone, superoxide dismutase (SOD), malondialdehyde (MDA), and acrosin activity were measured by ELISA. The cell apoptosis of testicular was observed by TUNEL. The sperm motility was analyzed by CASA. The results showed that the harms caused by W-EMP exposure, and the subsequent recovery were dependent on the intensity and pulse number of W-EMP. With the increase of electric intensity and pulse number the impact of W-EMP exposure increased, which leaded to a slower recovery rate. The results indicates that compared with the pulse number, the electric intensity in present work has much stronger impact on the reproductive system.

关键词

宽频谱电磁脉冲生殖系统精子活力血清睾酮顶体酶

Keywords

Wide-band electromagnetic pulseReproductive systemSperm motilitySerum testosteroneAcrosin

references

李海娟 , 胡向军 , 王丽峰 , 等 . 生物电磁学研究的新进展 . 生物物理学报 , 2013 . 29 (4 ):310 -318 . http://www.cnki.com.cn/Article/CJFDTOTAL-SWWL201304005.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SWWL201304005.htm, .

Haijuan LI , Xiangjun HU , Lifeng WANG , 等 . The new progress in the study of biological electromagnetism . Acta Biophysica Sinica , 2013 . 29 (4 ):310 -318 . http://www.cnki.com.cn/Article/CJFDTOTAL-SWWL201304005.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SWWL201304005.htm, .

P C Gailey , C E Easterly . Cell Membrane Potentials Induced During Exposure to EMP Fields . Electromagnetic Biology & Medicine , 1994 . 13 (2 ):159 -165 . DOI:10.3109/15368379409030710http://doi.org/10.3109/15368379409030710 .

M M Ragy . Effect of exposure and withdrawal of 900-MHz-electromagnetic waves on brain, kidney and liver oxidative stress and some biochemical parameters in male rats . Electromagnetic Biology and Medicine , 2014 . DOI:10.3109/15368378.2014.906446http://doi.org/10.3109/15368378.2014.906446 .

S M Mahdavi , H Sahraei , P Yaghmaei , 等 . Effects of electromagnetic radiation exposure on stress-related behaviors and stress hormones in male wistar rats . Biomolecules and Therapeutics , 2014 . 22 (6 ):570 -576 . DOI:10.4062/biomolther.2014.054http://doi.org/10.4062/biomolther.2014.054 .

王亚峰 , 苗霞 , 杨明娟 , 等 . 电磁脉冲暴露对雄性BALB/c幼鼠生殖系统发育的影响 . 辐射研究与辐射工艺学报 , 2013 . 31 (5 ):050201 DOI:10.11889/j.1000-3436.2013.rrj.31.050201http://doi.org/10.11889/j.1000-3436.2013.rrj.31.050201 .

Yafeng WANG , Xia MIAO , Mingjuan YANG , 等 . Effects of electromagnetic pulse exposure on the development of reproduction system of male BALB/c mice . Journal of Radiation Research and Radiation Processing , 2013 . 31 (5 ):050201 DOI:10.11889/j.1000-3436.2013.rrj.31.050201http://doi.org/10.11889/j.1000-3436.2013.rrj.31.050201 .

Schoenbach K H, Baum C E, Joshi R P, et al. A scaling law for bioelectric effects of nanosecond pulses[J]. 2008, Note 2, Bioelectric notes:1-25. DOI:10.1109/TDEI.2009. 5293932.

S Kumar , J Behari , R Sisodia . Influence of electromagnetic fields on reproductive system of male rats . International Journal of Radiation Biology , 2013 . 89 (3 ):147 -154 . DOI:10.3109/09553002.2013.741282http://doi.org/10.3109/09553002.2013.741282 .

J Behari , K K Kesari . Effects of microwave radiations on reproductive system of male rats . Embryo Talk , 2006 . 1 (Suppl 1 ):81 -85. .

K K Kesari , J Behari . Effects of microwave at 2.45 GHz radiations on reproductive system of male rats . Toxicological and Environ Chemistry , 2010 . 92 (6 ):1135 -1147 . DOI:10.1080/02772240903233637http://doi.org/10.1080/02772240903233637 .

H O Nisbet , C Nisbet , A Akar , 等 . Effects of exposure to electromagnetic field (1.8/0.9 GHz) on testicular function and structure in growing rats . Research in Veterinary Science , 2012 . 93 (2 ):1001 -1005 . DOI:10.1016/j.rvsc.2011.10.023http://doi.org/10.1016/j.rvsc.2011.10.023 .

J Martinez-Samano , P V Torres-Duran , M A Juarez-Oropeza , 等 . 2010. Effects of acute electromagnetic field exposure and movement restraint on antioxidant system in liver, heart, kidney and plasma of wistar rats:a preliminary report . International Journal of Radiation Biology , 2010 . 86 1088 -1094 . DOI:10.3109/09553002.2010.501841http://doi.org/10.3109/09553002.2010.501841 .

曾丽华 , 郭国祯 , 任东青 , 等 . 高功率微波辐照对雄性大鼠生殖细胞的影响 . 强激光与粒子束 , 2005 . 17 (8 ):1267 -1271 . http://www.cnki.com.cn/Article/CJFDTOTAL-QJGY200508036.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-QJGY200508036.htm, .

Lihua ZENG , Guozhen GUO , Dongqing REN , 等 . High power microwave irradiation effects on sperm cells of male rats . High Power Laser and Particle Beams , 2005 . 17 (8 ):1267 -1271 . http://www.cnki.com.cn/Article/CJFDTOTAL-QJGY200508036.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-QJGY200508036.htm, .

张欢 , 唐宇 , 张杰 , 等 . 脉冲微波对雄性大鼠生殖系统及其子代的影响 . 强激光与粒子束 , 2015 . 27 079001 DOI:10.11884/HPLPB201527.079001http://doi.org/10.11884/HPLPB201527.079001 .

Huan ZHANG , Yu TANG , Jie ZHANG , 等 . Effects of pulse microwave on reproductive system of male rat and its offspring . High Power Laser and Particle Beams , 2015 . 27 079001 DOI:10.11884/HPLPB201527.079001http://doi.org/10.11884/HPLPB201527.079001 .

Weixia Duan , Chuan Liu , Hongjuan Wu , 等 . Effects of exposure to extremely low frequency magnetic fields on spermatogenesis in adult rats . Bioelectromagnetics , 2014 . 35 (1 ):58 -69 . DOI:10.1002/bem.21816http://doi.org/10.1002/bem.21816 .

V Almasiova , K Holovska , V Cigankova , 等 . Structural and ultrastructural study of rat testes influenced by electromagnetic radiation . Journal of Toxicology and Environmental Health , 2014 . 77 (13 ):747 -750 . DOI:10.1080/15287394.2014.890988http://doi.org/10.1080/15287394.2014.890988 .

林锦文 , 刘合 , 张杰 , 等 . 电磁脉冲对大鼠心脏和肝脏损伤及修复的影响 . 辐射研究与辐射工艺学报 , 2015 . 33 (6 ):9 -16 . DOI:10.11889/j.1000-3436.2015.rrj.33http://doi.org/10.11889/j.1000-3436.2015.rrj.33 .

Jinwen LIN , He LIU , Jie ZHANG , 等 . Effects of wide band electromagnetic pulse on heart and liver injury and repair in rats . Journal of Radiation Research and Radiation Processing , 2015 . 33 (6 ):9 -16 . DOI:10.11889/j.1000-3436.2015.rrj.33.060201http://doi.org/10.11889/j.1000-3436.2015.rrj.33.060201 .

K A Bruqqink , W Jonqbloed , E A Biemans , 等 . Amyloid-β oligomer detection by ELISA in cerebrospinal fluid and brain tissue . Analytical Biochemistry , 2013 . 433 (2 ):112 -120 . DOI:10.1016/j.ab.2012.09.014http://doi.org/10.1016/j.ab.2012.09.014 .

L Maree , der Horst G Van . Quantification and identification of sperm subpopulations using computeraided sperm analysis and species-specific cut-off values for swimming speed . Biotechnic and Histochemistry , 2013 . 88 (3-4 ):181 -193 . DOI:10.3109/10520295.2012.757366http://doi.org/10.3109/10520295.2012.757366 .

N Zribi , N F Chakroun , F B Abdallah , 等 . Effect of freezing-thawing process and quercetin on human sperm survival and DNA integrity . Cryobiology , 2012 . 65 (3 ):326 -331 . DOI:10.1016/j.cryobiol.2012.09.003http://doi.org/10.1016/j.cryobiol.2012.09.003 .

B Zelezoa . Teolation of a form into-form . Biological Chemistry Hoppe-Seyler , 1989 . 370 323 -327 . DOI:10.1515/bchm3.1989.370.1.323http://doi.org/10.1515/bchm3.1989.370.1.323.

黄平治 , 李永海 . 男性不育 , :北京科学技术文献出版社 , 1990 . 73 .

Pingzhi HUANG , Yonghai LI . Male sterility , :Beijing Science Technology Literature Press , 1990 . 73 .

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Cumulative effect of wide-band electromagnetic pulse exposure on the reproductive system of male rats

电磁脉冲对大鼠心脏和肝脏损伤及修复的影响

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Laboratory of Biophysics, Department of Physics and Materials Science, East China Normal University

1(Institute of Biophysics, Department of Physics, East China Normal University,,)

2(State Key Laboratory of Precision Spectroscopy, East China Normal University,,)

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