Study on screening and mutation breeding of high-yield <italic style="font-style: italic">Lactobacillus plantarum</italic> by heavy ion irradiation

Yuchen WANG; Shuyang WANG; Miaoyin DONG; Fuqiang XU; Wei HU; Jihong CHEN; Wenjian LI; Shunyu HAN; Jing LIU

doi:10.11889/j.1000-3436.2017.rrj.35.010401

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Study on screening and mutation breeding of high-yield Lactobacillus plantarum by heavy ion irradiation

RADIATION TECHNOLOGY APPLICATION | 更新时间：2021-12-10

- Study on screening and mutation breeding of high-yield Lactobacillus plantarum by heavy ion irradiation
- Journal of Radiation Research and Radiation Processing Vol. 35, Issue 1, Pages: 10401(2017)
- 作者机构：
  
  1.甘肃农业大学食品科学与工程学院兰州 730070
  2.中国科学院近代物理研究所生物物理室兰州 730000
  3.甘肃省辐照诱变育种工程实验室兰州 730000
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(11305225);Science and Technology Service Network Initiative(KFJ-EW-STS-086);Xizang forage Science and Technology(Y106460XZO)
- DOI：10.11889/j.1000-3436.2017.rrj.35.010401
  CLC：
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Yuchen WANG, Shuyang WANG, Miaoyin DONG, et al. Study on screening and mutation breeding of high-yield Lactobacillus plantarum by heavy ion irradiation. [J]. Journal of Radiation Research and Radiation Processing 35(1):10401(2017)
DOI：

Yuchen WANG, Shuyang WANG, Miaoyin DONG, et al. Study on screening and mutation breeding of high-yield Lactobacillus plantarum by heavy ion irradiation. [J]. Journal of Radiation Research and Radiation Processing 35(1):10401(2017) DOI： 10.11889/j.1000-3436.2017.rrj.35.010401.

摘要

采用重离子束,12,C,6+,对乳酸菌出发菌株JTL进行辐照选育。确定吸收剂量300 Gy时最优，在该吸收剂量下，出发菌种的正突变率和致死率均达到最高，分别为34.4%、80%。通过酸斑法初筛和发酵复筛法，选育出高产酸的3株突变菌株（JTL2、JTL3、JTL5），其发酵液中乳酸含量比出发菌株JTL提高了41.29%~49.64%。同时，经过传代实验证实，3株菌株具有良好的遗传稳定性。研究表明，利用重离子束,12,C,6+,辐照处理乳酸菌可以得到高产酸的突变菌株，诱变效果显著。

Abstract

12,C,6+, ion beam was used to irradiate strains of ,Lactobacillus plantarum, JTL, and the results showed that the strain could get the highest positive mutation rate and lethality with 34.4% and 80% at the optimal absorbed dose of 300 Gy. The strains which could produce higher lactic acid were screened by selective MRS mediums and fermentation experiments. Finally, three mutations JTL2, JTL3 and JTL5 were obtained through the two screening steps of selecting plate and fermentation, and the contents of lactic acid in this three fermentation broth were greater than the original strain by 41.29% to 49.64%. The pass-generation test indicated that the heredity character of these mutations were stable. It is demonstrated that the lactic acids produced by ,Lactobacillus plantarum, JTL were remarkably improved, and heavy ion beam is an efficient radiation resource for microorganism breeding.

关键词

乳酸菌重离子束辐照诱变育种

Keywords

Lactobacillus plantarumHeavy ion beamIrradiationMutation breeding

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1(College of Life Science, Northwest Normal University,,)

2 (Institute of Modern Physics, Chinese Academy of Sciences,,)

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Study on screening and mutation breeding of high-yield Lactobacillus plantarum by heavy ion irradiation

DOI：10.11889/j.1000-3436.2017.rrj.35.010401

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