DNA double-strand breaks induced by X-ray irradiation in human glioma cells

Shuyan PEI; Jin ZHAO; Ying MA; Dan ZHANG; Cong NIE; Lili WANG; Zhong GUO

doi:10.11889/j.1000-3436.2017.rrj.35.040201

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DNA double-strand breaks induced by X-ray irradiation in human glioma cells

RADIBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- DNA double-strand breaks induced by X-ray irradiation in human glioma cells
- Journal of Radiation Research and Radiation Processing Vol. 35, Issue 4, (2017)
- 作者机构：
  
  1.西北民族大学医学院兰州 730030
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(81560508);the National Natural Science Foundation of China(31560254);Program for Leading Talent of SEAC([2016]57);National Undergraduate Training Programs for Innovation and Entrepreneurship(201610742002);National Undergraduate Training Programs for Innovation and Entrepreneurship(201710742101)
- DOI：10.11889/j.1000-3436.2017.rrj.35.040201
  CLC：
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Shuyan PEI, Jin ZHAO, Ying MA, et al. DNA double-strand breaks induced by X-ray irradiation in human glioma cells. [J]. Journal of Radiation Research and Radiation Processing 35(4):040201-8(2017)
DOI：

Shuyan PEI, Jin ZHAO, Ying MA, et al. DNA double-strand breaks induced by X-ray irradiation in human glioma cells. [J]. Journal of Radiation Research and Radiation Processing 35(4):040201-8(2017) DOI： 10.11889/j.1000-3436.2017.rrj.35.040201.

摘要

采用免疫荧光染色法检测X射线诱导M059K细胞磷酸化组蛋白H2AX（γH2AX）和磷酸化的毛细血管扩张性共济失调症突变基因（pATM）焦点的形成；流式细胞仪分析M059K细胞γH2AX和pATM表达的周期依赖性。免疫荧光染色检测结果显示，γH2AX和pATM焦点阳性细胞分别以照射后0.5、4 h表达最高，照射后24 h，高剂量组焦点阳性细胞达100%。流式细胞仪分析结果显示，γH2AX和pATM的表达具有细胞周期依赖性。照射后0.5、4 h，γH2AX和pATM在细胞各期的表达均明显增加，并以G0/G1期为著；照射后24 h，γH2AX和pATM在G0/G1和S期表达降低，而G2/M期细胞表达明显增加。细胞呈现明显的G2/M期阻滞。结果提示，γH2AX和ATM信号通路参与X射线诱导的M059K细胞DNA双链断裂。

Abstract

The foci of phosphorylated histone H2AX (γH2AX) and phosphorylated ataxia telangiectasia mutated (pATM) were visualized using immunocytochemical methods. Flow cytometry was used to assay γH2AX and pATM expression and the cell cycle. The results of immunocytochemical assay showed that the strongest induction of γH2AX and pATM foci appeared at 0.5 h and 4 h after irradiation, and the positivity for γH2AX and pATM foci reached 100% at 24 h with X-ray exposure in higher-dose groups. Flow cytometry results showed that the expression of γH2AX and pATM was related to the cell cycle. The rise of γH2AX and pATM expression was shown in each phase at 0.5 h and 4 h post-exposure, and this increase was more obvious in G0/G1 phase. At 24 h post-exposure, the expression of γH2AX and pATM decreased in G0/G1 and S phase, while increased significantly in G2/M phase. There was significant prevalence of the arrest at the G2–M transition among M059K cells. The above results indicate that the signaling pathways of γH2AX and pATM is involved in X-ray-induced double-strand breaks in M059K cells.

关键词

X射线辐照M059K细胞DNA双链损伤γH2AXpATM

Keywords

X-ray irradiationM059KDNA double-strand breakγH2AXpATM

references

沈筱筠 , 闫春兰 , 杨军 . DNA双链断裂诱导的细胞损伤应答反应研究进展 . 中国药理学与毒理学杂志 , 2010 . 24 (6 ):539 -542 . DOI:10.3867/j.issn.1000-3002.2010.06.018.SHENhttp://doi.org/10.3867/j.issn.1000-3002.2010.06.018.SHEN .

Xiaojun SHEN , Chunlan YAN , Jun YANG . Progress in cellular responses to DNA double strands breaks . Chinese Journal of Pharmacology and Toxicology , 2010 . 24 (6 ):539 -542 . DOI:10.3867/j.issn.1000-3002.2010.06.018http://doi.org/10.3867/j.issn.1000-3002.2010.06.018 .

J Reindl , S Girst , D W Walsh , 等 . Chromatin organization revealed by nanostructure of irradiation induced γH2AX, 53BP1 and Rad51 foci . Scientific Reports , 2017 . 7 40616 DOI:10.1038/srep40616http://doi.org/10.1038/srep40616 .

E P Rogakou , D R Pilch , A H Orr , 等 . DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139 . Journal of Biological Chemistry , 1998 . 273 (10 ):5858 -5868 . DOI:10.1074/jbc.273.10.5858http://doi.org/10.1074/jbc.273.10.5858 .

R Okayasu . Repair of DNA damage induced by accelerated heavy ions-a mini review . International Journal of Cancer , 2012 . 130 991 -1000 . DOI:10.1002/ijc.26445http://doi.org/10.1002/ijc.26445 .

赵晋 , 郭忠 , 马建秀 . γH2AX和ATM与内源性氧化剂所致DNA损伤关系的研究进展 . 基础医学与临床 , 2010 . 30 (4 ):437 -441 . http://www.cnki.com.cn/Article/CJFDTOTAL-JCYL201004038.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-JCYL201004038.htm, .

in J , Zhong GUO , Jianxiu MA . Progress in the research of relationship between γH2AX, ATM and DNA damage by endogenous oxidants . Basic and Clinical Medicine , 2010 . 30 (4 ):437 -441 . http://www.cnki.com.cn/Article/CJFDTOTAL-JCYL201004038.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-JCYL201004038.htm, .

H Y Zhang , L L Sun , K Wang , 等 . Loss of H3K9me3 correlates with ATM activation and histone H2AX phosphorylation deficiencies in hutchinson-gilford progeria syndrome . PLoS One , 2016 . 11 (12 ):e0167454 DOI:10.1371/journal.pone.0167454http://doi.org/10.1371/journal.pone.0167454 .

A L Elaimy , A Ahsan , K Marsh , 等 . ATM is the primary kinase responsible for phosphorylation of Hsp90α after ionizing radiation . Oncotarget , 2016 . 7 (50 ):82450 -82457 . DOI:10.18632/oncotarget.12557http://doi.org/10.18632/oncotarget.12557 .

J Zhao , Z Guo , H Zhang , 等 . The potential value of the neutral comet and γH2AX foci assays in assessing radiosensitivity to carbon ion beams radiation therapy in human tumor cell lines . Radiology and Oncology , 2013 . 47 (3 ):247 -257 . DOI:10.2478/raon-2013-0045http://doi.org/10.2478/raon-2013-0045 .

Z Guo , J Zhao , L Song , 等 . Induction of H2AX phosphorylation in tumor cells by gossypol acetic acid is mediated by phosphatidylinositol 3-kinase (PI3K) family . Cancer Cell International , 2014 . 14 141 DOI:10.1186/s12935-014-0141-5http://doi.org/10.1186/s12935-014-0141-5 .

B Sadikovic , D I Rodenhiser . Benzopyrene exposure disrupts DNA methylation and growth dynamics in breast cancer cells . Toxicology and Applied Pharmacology , 2006 . 216 (3 ):458 -468 . DOI:10.1016/j.taap.2006.06.012http://doi.org/10.1016/j.taap.2006.06.012 .

郭传玲 , 王菊芳 , 李文建 , 等 . 三种肿瘤细胞对γ射线辐射敏感性的比较 . 同位素 , 2005 . 18 (3 ):164 -166 . http://www.cnki.com.cn/Article/CJFDTOTAL-TWSZ200503009.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-TWSZ200503009.htm, .

Chuanling GUO , Jufang WANG , Wenjian LI , 等 . Comparision of the radiosensitivity among three kinds of tumor cells irradiated with γ rays . Journal of Isotopes , 2005 . 18 (3 ):164 -166 . http://www.cnki.com.cn/Article/CJFDTOTAL-TWSZ200503009.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-TWSZ200503009.htm, .

郭忠 , 赵晋 , 薛同敏 , 等 . 醋酸棉酚诱导人粘液表皮样癌MEC-1细胞DNA双链断裂 . 生理学报 , 2011 . 63 (2 ):164 -170 . http://www.cnki.com.cn/Article/CJFDTOTAL-SLXU201102012.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SLXU201102012.htm, .

Zhong GUO , Jin ZHAO , Tongmin XUE , 等 . Gossypol acetic acid induces DNA double-strand breaks in human mucoepidermoid carcinoma cell MEC-1 . Acta Physiologica Sinica , 2011 . 63 (2 ):164 -170 . http://www.cnki.com.cn/Article/CJFDTOTAL-SLXU201102012.htmhttp://www.cnki.com.cn/Article/CJFDTOTAL-SLXU201102012.htm, .

刘辉 , 张红 , 刘兵 , 等 . P53及其相关蛋白对X射线照射肝癌细胞周期的调节 . 原子核物理评论 , 2007 . 24 (4 ):289 -293 . DOI:10.11804/NuclPhysRev.24.04.289http://doi.org/10.11804/NuclPhysRev.24.04.289.

Hui LIU , Hong ZHANG , Bing LIU , 等 . Effect of X-ray radiationon cell cycle regulated by P53 and its related proteins in HepG2 cell . Nuclear Physics Review , 2007 . 24 (4 ):289 -293 . DOI:10.11804/NuclPhysRev.24.04.289http://doi.org/10.11804/NuclPhysRev.24.04.289.

胡国清 , 孙银萍 . 放射线对体外培养鼻咽癌细胞细胞周期阻滞、凋亡和抑癌基因p57kip2表达的影响 . 华中科技大学学报(医学版) , 2005 . 34 (6 ):741 -743 . http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFD2005&filename=TJYX200506025&v=MjU2OTNZOUhZWVI4ZVgxTHV4WVM3RGgxVDNxVHJXTTFGckNVUkwyZllPVnVGaURoVTc3QU1TZlNkckc0SHRUTXE=http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFD2005&filename=TJYX200506025&v=MjU2OTNZOUhZWVI4ZVgxTHV4WVM3RGgxVDNxVHJXTTFGckNVUkwyZllPVnVGaURoVTc3QU1TZlNkckc0SHRUTXE=, .

Guoqing HU , Yinping SUN . Effect of radioactive rays on cell cycle retardation, apoptosis and the expression of antioncogene p57kip2 in cultured nasopharyngeal carcinoma cell line in vitro . Acta Medicinae Universitatis Scientiae et Technologiae Huazhong , 2005 . 34 (6 ):741 -743 . http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFD2005&filename=TJYX200506025&v=MjU2OTNZOUhZWVI4ZVgxTHV4WVM3RGgxVDNxVHJXTTFGckNVUkwyZllPVnVGaURoVTc3QU1TZlNkckc0SHRUTXE=http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFD2005&filename=TJYX200506025&v=MjU2OTNZOUhZWVI4ZVgxTHV4WVM3RGgxVDNxVHJXTTFGckNVUkwyZllPVnVGaURoVTc3QU1TZlNkckc0SHRUTXE=, .

孙银萍 , 胡国清 . 放射线对鼻咽癌细胞周期阻滞、凋亡和抑癌基因p57kip2表达的影响 . 临床肿瘤学杂志 , 2008 . 13 (6 ):491 -494 . http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFD2008&filename=LCZL200806004&v=Mjk3NjZHNEh0bk1xWTlGWUlSOGVYMUx1eFlTN0RoMVQzcVRyV00xRnJDVVJMMmZZT1Z1RmlEaFU3ekJLUzdSWXI=http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFD2008&filename=LCZL200806004&v=Mjk3NjZHNEh0bk1xWTlGWUlSOGVYMUx1eFlTN0RoMVQzcVRyV00xRnJDVVJMMmZZT1Z1RmlEaFU3ekJLUzdSWXI=, .

Yinping SUN , Guoqing HU . Research on cellcycl earrest, apoptosis and the expression of tumor suppressor gene p57kip2 by radiation in nasopharyngeal carcinoma cell line in vitro . Chinese Clinical Oncology , 2008 . 13 (6 ):491 -494 . http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFD2008&filename=LCZL200806004&v=Mjk3NjZHNEh0bk1xWTlGWUlSOGVYMUx1eFlTN0RoMVQzcVRyV00xRnJDVVJMMmZZT1Z1RmlEaFU3ekJLUzdSWXI=http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFD2008&filename=LCZL200806004&v=Mjk3NjZHNEh0bk1xWTlGWUlSOGVYMUx1eFlTN0RoMVQzcVRyV00xRnJDVVJMMmZZT1Z1RmlEaFU3ekJLUzdSWXI=, .

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