Screening and mutation breeding of high-yield <italic style="font-style: italic">Cordyceps militaris</italic> via heavy ion irradiation

Guojun JIA; Zhengui MA; Fengwu ZHAO; Xihong LU; Guirong WANG

doi:10.11889/j.1000-3436.2017.rrj.35.060401

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Screening and mutation breeding of high-yield Cordyceps militaris via heavy ion irradiation

RADIATION TECHNOLOGY APPLICATION | 更新时间：2021-12-10

- Screening and mutation breeding of high-yield Cordyceps militaris via heavy ion irradiation
- Journal of Radiation Research and Radiation Processing Vol. 35, Issue 6, (2017)
- 作者机构：
  
  1.兰州职业技术学院生物工程系兰州 730070
  2.中国科学院近代物理研究所兰州 730000
  3.甘肃农业大学动物医学院兰州 730070
- 作者简介：
- 基金信息：
  
  Lanzhou talent innovation and entrepreneurship project(2015-RC-21);Lanzhou science and technology plan project(2016-3-2);Lanzhou Vocational Technical College project(2016B-1)
- DOI：10.11889/j.1000-3436.2017.rrj.35.060401
  CLC：
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Guojun JIA, Zhengui MA, Fengwu ZHAO, et al. Screening and mutation breeding of high-yield Cordyceps militaris via heavy ion irradiation. [J]. Journal of Radiation Research and Radiation Processing 35(6):060401-7(2017)
DOI：

Guojun JIA, Zhengui MA, Fengwu ZHAO, et al. Screening and mutation breeding of high-yield Cordyceps militaris via heavy ion irradiation. [J]. Journal of Radiation Research and Radiation Processing 35(6):060401-7(2017) DOI： 10.11889/j.1000-3436.2017.rrj.35.060401.

摘要

利用,12,C,6+,重离子束对北冬虫夏草出发菌株的菌悬液进行辐照诱变处理，采用平板分离法初筛变异菌株，通过培养子实体复筛高产菌株，并测定子实体的产量、多糖和虫草素含量。结果表明，在吸收剂量为150Gy时，出发菌株的致死率达到最高为82%；经过初筛和复筛，最终选育出3株高产子实体突变菌株G2、G5、G12，并经连续传代10次，测定子实体产量稳定在8.02~8.21 g/瓶，与出发菌株的子实体产量相比提高了52.47%~56.08%，且稳定性保持良好。研究表明，利用重离子束辐照诱变选育北冬虫夏草，可以获得高产子实体菌株。

Abstract

Strains of ,Cordyceps militaris, grown in suspension culture were irradiated by ,12,C,6+, ion beam, screened using the plate separation method, and re-screened by culturing fruiting bodies to determine the fruiting-body yield, polysaccharide and cordycepin content. The strains displayed the highest lethality,i.e,., 82%, at the optimal absorbed dose of 150 Gy. Three mutants (G2, G5, G12) were obtained, which could produce a greater number of fruiting bodies. Meanwhile, the ten times pass-generation test indicated that the fruiting bodies stabilized at 8.02 to 8.21 g/bottle in these three mutants, which were 52.47% to 56.08% higher than the original strain, and the hereditary characters of these mutants were stable. The present findings show that a high-yield fruiting body of ,C.militaris, can be obtained by mutation breeding using a heavy ion beam irradiation.

关键词

北冬虫夏草重离子束诱变育种辐照

Keywords

Cordyceps militarisHeavy ion beamsMutation breedingIrradiation

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College of Food Science and Engineering, Gansu Agriculture University

Institute of Modern Physics, Chinese Academy of Sciences

Irradiation Mutation Breeding Engineering Laboratory of Gansu Province

1(College of Life Science, Northwest Normal University,,)

2 (Institute of Modern Physics, Chinese Academy of Sciences,,)

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Screening and mutation breeding of high-yield Cordyceps militaris via heavy ion irradiation

DOI：10.11889/j.1000-3436.2017.rrj.35.060401

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