Cumulative effect of wide-band electromagnetic pulse exposure on the reproductive system of male rats

Mingjia LEI; He LIU; Jie ZHANG; Hongxin QI; Xianghui WANG

doi:10.11889/j.1000-3436.2018.rrj.36.010202

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Cumulative effect of wide-band electromagnetic pulse exposure on the reproductive system of male rats

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- Cumulative effect of wide-band electromagnetic pulse exposure on the reproductive system of male rats
- Journal of Radiation Research and Radiation Processing Vol. 36, Issue 1, (2018)
- 作者机构：
  
  1.华东师范大学物理与材料科学学院生物物理实验室上海 200241
- 作者简介：
- 基金信息：
  
  National Natural Science Foundation of China(31600675)
- DOI：10.11889/j.1000-3436.2018.rrj.36.010202
  CLC：
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Mingjia LEI, He LIU, Jie ZHANG, et al. Cumulative effect of wide-band electromagnetic pulse exposure on the reproductive system of male rats. [J]. Journal of Radiation Research and Radiation Processing 36(1):010202-9(2018)
DOI：

Mingjia LEI, He LIU, Jie ZHANG, et al. Cumulative effect of wide-band electromagnetic pulse exposure on the reproductive system of male rats. [J]. Journal of Radiation Research and Radiation Processing 36(1):010202-9(2018) DOI： 10.11889/j.1000-3436.2018.rrj.36.010202.

摘要

在不同宽频电磁脉冲辐照条件（场强,E,=110、297、873 kV/m，每个场强下的脉冲个数,N,=5、10次）下周期性（2次/d）辐照雄性大鼠两周（共28次），辐照结束后分别于6 h、1、3、7、10 d取样检测大鼠的血清睾酮含量、睾丸组织中超氧化物歧化酶（Superoxidase dismutase，SOD）与丙二醛（Malondialdehyde，MDA）含量及生精细胞凋亡率、大鼠的精子顶体酶活性、精子存活率等生物指标，通过与相同辐照条件下的单次辐照结果进行对比，考察多次重复辐照对雄性大鼠生殖系统的累积损伤效应及随后的修复情况。结果表明，多次重复辐照对雄性大鼠生殖系统具有累积损伤作用，其累积效果与单次辐照剂量有关，场强越高，脉冲个数越多，累积损伤效应越明显。与单次辐照损伤类似，累积损伤可随照后时间的延长得到一定程度的修复。

Abstract

The present work focused on the cumulative damage caused by periodically repeated wide-band electromagnetic pulses. The exposure protocol was as follows: electric field intensity ,E,=110 kV/m, 297 kV/m, and 873 kV/m; pulse number ,N,=5, and 100; exposure was repeated 2 times per day, for 2 weeks. 6 h, 1 d, 3 d, 7 d, and 10 d after exposure, the testosterone concentration in blood plasma, superoxide dismutase (SOD) and malondialdehyde (MDA) in the testis, and acrosin activity of sperms were determined by ELISA. The apoptosis rate of spermatogenic cells in the testis was studied by TUNEL, and the survival rate of sperms in the epididymis by CASA. The results showed that compared with single exposure, 28 periodic exposures exhibited a cumulative damaging effect on the reproductive system of male rats. The cumulative effect increased significantly with increasing ,E, and ,N,. Similar to single exposure, the damage caused by repetitive exposure was gradually recovered to a certain degree with prolongation of exposure time.

关键词

宽频电磁脉冲重复辐照累积损伤生殖系统精子

Keywords

Wide-band electromagnetic pulseRepeated exposureCumulative damageReproductive systemSperm

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