Screening for high teicoplanin-producing strain by mutation combined with resistance to self-produced metabolite

Yin ZHANG; Zhulan ZHANG; Guanrong QIU; Huangjian YANG; Desen WANG; Linying REN; Yunyang LIAN

doi:10.11889/j.1000-3436.2018.rrj.36.010401

您当前的位置：

首页 >

文章列表页 >

Screening for high teicoplanin-producing strain by mutation combined with resistance to self-produced metabolite

RADIATION TECHNOLOGY APPLICATION | 更新时间：2021-12-10

- Screening for high teicoplanin-producing strain by mutation combined with resistance to self-produced metabolite
- Journal of Radiation Research and Radiation Processing Vol. 36, Issue 1, Pages: 10401(2018)
- 作者机构：
  
  1.福建省微生物研究所福建省新药(微生物)筛选重点实验室福州 350007
- 作者简介：
- 基金信息：
  
  Major Science and Technology Project of Fujian Province of China(2014YZ0004-2);Major Research and Development Platform for Chemical Technology of Fujian Province of China(2014Y2001);Bio-Pharmaceutical Industry Technology Innovation Center of Fuzhou City of China(2016-PT-36);Special Project of Public Welfare Research Institute of Fujian Province of China(2017R1009-13)
- DOI：10.11889/j.1000-3436.2018.rrj.36.010401
  CLC：
扫描看全文
Yin ZHANG, Zhulan ZHANG, Guanrong QIU, et al. Screening for high teicoplanin-producing strain by mutation combined with resistance to self-produced metabolite. [J]. Journal of Radiation Research and Radiation Processing 36(1):10401(2018)
DOI：

Yin ZHANG, Zhulan ZHANG, Guanrong QIU, et al. Screening for high teicoplanin-producing strain by mutation combined with resistance to self-produced metabolite. [J]. Journal of Radiation Research and Radiation Processing 36(1):10401(2018) DOI： 10.11889/j.1000-3436.2018.rrj.36.010401.

摘要

采用,60,Co γ-射线辐射结合抗性筛选技术，对替考拉宁产生菌替考游动放线菌FIM-16的孢子进行诱变处理，以替考拉宁自身抗性作为选择压力，并通过摇瓶发酵对抗性基因突变株进行筛选。结果获得一株摇瓶发酵水平较出发菌株FIM-16提高两倍的高产菌FIM-16-58，且遗传性状相对稳定，菌种传3代发酵水平无明显影响，菌种斜面在4 ℃稳定保藏两个月；其在1 T发酵罐上发酵，添加0.1%的玉米油可有效抑制泡沫的产生，替考拉宁产量达5.0 g/L以上。

Abstract

To screen high-yield teicoplanin-producing strain,60,Co γ-ray irradiated spore suspension of the strain ,Actinoplanes teichomyceticus, FIM-16 was spread on regenerating plate containing lethal concentration of teicoplanin. Teicoplanin-resistant mutants were picked and screened by shake-flask fermentation. Strain FIM-16-58 showed two times higher teicoplanin yield than the original strain, and demonstrated higher hereditary stability. There was no obvious effect on the fermentation level of the strain over three generations, and its slope was stable at 4 ℃ for two months. During scale-up experiment in the 1 T fermenter, foam was suppressed effectively by adding 0.1% corn oil, and the yield of teicoplanin reached over 5.0 g/L. Therefore, the high-yield strain laid the foundation of its industrial production and fermentation.

关键词

替考游动放线菌抗性突变筛选辐射诱变替考拉宁

Keywords

Actinoplanes teichomyceticusResistance mutation screeningRadiation mutagenesisTeicoplanin

references

A Borghi , P Antonini , M Zanol , a1 et . Isolation and structure deterination of two new analogs of teicoplanin, a glycopetide antibiotic . Joumal of Antibiotics , 1989 . 42 (3 ):361 -366 . DOI:10.7164/antibiotics.42.361http://doi.org/10.7164/antibiotics.42.361 .

E S R Darley , A P Macgowan . The use and therapeutic drug monitoring of teicoplanin in the UK . Clinical Microbiology and Infection , 2004 . 10 (1 ):62 -69 . DOI:10.1111/j.1469-0691.2004.00747.xhttp://doi.org/10.1111/j.1469-0691.2004.00747.x .

夏振强 , 古勇 , 官家发 . 糖肽类抗生素索替考拉宁研究进展 . 天然产物研究与开发 , 2005 . 18 (2 ):236 -238 . DOI:10.3969/j.issn.1001-6880.2005.02.032http://doi.org/10.3969/j.issn.1001-6880.2005.02.032 .

Zhenqiang XIA , Yong GU , Jiafa GUAN . Study of teicoplanin-a glycopepdite antibiotic . Natural Product Research and Development , 2005 . 18 (2 ):236 -238 . DOI:10.3969/j.issn.1001-6880.2005.02.032http://doi.org/10.3969/j.issn.1001-6880.2005.02.032 .

何礼贤 , 潘钰 , 陈世耀 , 等 . 替考拉宁治疗革兰阳性球菌感染的临床研究 . 中华内科杂志 , 2005 . 44 (5 ):337 -340 . DOI:10.3760/j.issn:0578-1426.2005.05.007http://doi.org/10.3760/j.issn:0578-1426.2005.05.007 .

Lixian HE , Jue PAN , Shiyao CHEN , 等 . Clinical study of teicoplanin in the treatment of patients with gram-positive cocci: the Chinese experience . Chinese Journal of Internal Medicine , 2005 . 44 (5 ):337 -340 . DOI:10.3760/j.issn:0578-1426.2005.05.007http://doi.org/10.3760/j.issn:0578-1426.2005.05.007 .

王德森 , 张祝兰 , 唐文力 , 等 . 替考拉宁产生菌A. teichomycoticus FIM-37发酵条件优化 . 药物生物技术 , 2017 . 24 (4 ):310 -314 . DOI:10.19526/j.cnki.1005-8915.20170406http://doi.org/10.19526/j.cnki.1005-8915.20170406 .

Desen WANG , Zhulan ZHANG , Wenli TANG , 等 . Optimization of fermentation conditions of teicoplanin-producing strain A. teichomyceticus FIM-37 . Pharmaceutical Biotechnology , 2017 . 24 (4 ):310 -314 . DOI:10.19526/j.cnki.1005-8915.20170406http://doi.org/10.19526/j.cnki.1005-8915.20170406 .

徐加兵 , 魏晓东 , 那可 , 等 . 杆菌肽产生菌的诱变育种及培养基优化 . 中国医药工业杂志 , 2013 . 44 (5 ):446 -452 . DOI:10.16522/j.cnki.cjph.2013.05.038http://doi.org/10.16522/j.cnki.cjph.2013.05.038 .

Jiabing XU , Xiaodong WEI , Ke NA , 等 . Mutagenesis breeding and optimization for high production of bacitracinby bacillus licheniformis . Chinese Journal of Pharmaceuticals , 2013 . 44 (5 ):446 -448 . DOI:10.16522/j.cnki.cjph.2013.05.038.cnki.cjph.2013.05.038http://doi.org/10.16522/j.cnki.cjph.2013.05.038.cnki.cjph.2013.05.038 .

剡倩 , 张苗苗 , 郭晓鹏 , 等 . X射线诱变选育嗜酸氧化亚铁硫杆菌及其对难处理金矿浸出的研究 . 辐射研究与辐射工艺学报 , 2016 . 34 (5 ):050401 DOI:10.11889/j.1000-3436.2016.rrj.34.050401http://doi.org/10.11889/j.1000-3436.2016.rrj.34.050401 http://www.j.sinap.ac.cn/fushe/CN/Y2016/V34/I5/50401http://www.j.sinap.ac.cn/fushe/CN/Y2016/V34/I5/50401, .

Qian YAN , Miaomiao ZHANG , Xiaopeng GUO , 等 . Study on breeding acidithiobacillus ferrooxidans induced by X-ray irradiation and bioleaching of refractory gold ore . Journal of Radiation Research and Radiation Processing , 2016 . 34 (5 ):050401 DOI:10.11889/j.1000-3436.2016.rrj.34.050401http://doi.org/10.11889/j.1000-3436.2016.rrj.34.050401 http://www.j.sinap.ac.cn/fushe/CN/Y2016/V34/I5/50401http://www.j.sinap.ac.cn/fushe/CN/Y2016/V34/I5/50401, .

王雨辰 , 王曙阳 , 董妙音 , 等 . 重离子束辐照选育高产植物乳酸菌 . 辐射研究与辐射工艺学报 , 2017 . 35 (1 ):010401 DOI:10.11889/j.1000-3436.2017.rrj.35.010401http://doi.org/10.11889/j.1000-3436.2017.rrj.35.010401 http://www.j.sinap.ac.cn/fushe/CN/Y2017/V35/I1/10401http://www.j.sinap.ac.cn/fushe/CN/Y2017/V35/I1/10401, .

Yuchen WANG , Shuyang WANG , Miaoyin DONG , 等 . Study on screening and mutation breeding of high-yield Lactobacillus plantarum by heavy ion irradiation . Journal of Radiation Research and Radiation Processing , 2017 . 35 (1 ):10401 -010401 . DOI:10.11889/j.1000-3436.2017.rrj.35.010401http://doi.org/10.11889/j.1000-3436.2017.rrj.35.010401 http://www.j.sinap.ac.cn/fushe/CN/Y2017/V35/I1/10401http://www.j.sinap.ac.cn/fushe/CN/Y2017/V35/I1/10401, .

虞龙 , 姚驰亚 , 吴晓菲 , 等 . 低能氮离子注入和紫外线复合诱变选育高产酿酒酵母 . 辐射研究与辐射工艺学报 , 2017 . 35 (2 ):020401 DOI:10.11889/j.1000-3436.2017.rrj.35.020401http://doi.org/10.11889/j.1000-3436.2017.rrj.35.020401 http://www.j.sinap.ac.cn/fushe/CN/Y2017/V35/I2/20401http://www.j.sinap.ac.cn/fushe/CN/Y2017/V35/I2/20401, .

Long YU , Chiya YAO , Xiaofei WU , 等 . Mutation breeding of ethanol-producing yeast Saccharomyces cerevisiae by low-energy nitrogen ion implantation and UV rays . Journal of Radiation Research and Radiation Processing , 2017 . 35 (2 ):020401 DOI:10.11889/j.1000-3436.2017.rrj.35.020401http://doi.org/10.11889/j.1000-3436.2017.rrj.35.020401 http://www.j.sinap.ac.cn/fushe/CN/Y2017/V35/I2/20401http://www.j.sinap.ac.cn/fushe/CN/Y2017/V35/I2/20401, .

刘体颜 , 邢玉华 , 戴素琴 , 等 . 达托霉素生产菌(ATCC31568)的紫外诱变和抗生素抗性筛选 . 中国抗生素杂志 , 2013 . 38 (9 ):664 -669 . DOI:10.3969/j.issn.1001-8689.2013.09.005http://doi.org/10.3969/j.issn.1001-8689.2013.09.005 .

Tiyan LIU , Yuhua XING , Suqin DAI , 等 . Ultraviolet mutagenesis and resistance slelection of daptomycin-producing strain(ATCC31568) . Chinese Journal of Antibiotics , 2013 . 38 (9 ):664 -669 . DOI:10.3969/j.issn.1001-8689.2013.09.005http://doi.org/10.3969/j.issn.1001-8689.2013.09.005 .

王建玲 , 许铭玉 , 石天歌 , 等 . 紫外诱变选育恩拉霉素高产菌株 . 工业微生物 , 2015 . 45 (5 ):23 -28 . DOI:10.3969/j.issn.1001-6678.2015.05.005http://doi.org/10.3969/j.issn.1001-6678.2015.05.005 .

Jianling WANG , Mingyu XU , Tiange SHI , 等 . UV mutagenesis for high-yield enramycin producing stains . Industrial Microbiology , 2015 . 45 (5 ):23 -28 . DOI:10.3969/j.issn.1001-6678.2015.05.005http://doi.org/10.3969/j.issn.1001-6678.2015.05.005 .

杨芬 , 巢波 , 杨万红 , 等 . 一株抗生素和重金属交叉抗性菌的筛选及抗性研究 . 环境工程学报 , 2017 . 11 (2 ):1287 -1292 . DOI:10.12030/j.cjee.201509222http://doi.org/10.12030/j.cjee.201509222 .

Fen YANG , Bo CHAO , Wanhong YANG , 等 . Screening on resistance strains of antibiotic and heavy metal and its resistance research . Chinese Journal of Environmental Engineering , 2017 . 11 (2 ):1287 -1292 . DOI:10.12030/j.cjee.201509222http://doi.org/10.12030/j.cjee.201509222 .

王海霞 , 陈园 , 王超 , 等 . 组合抗生素抗性选育多杀菌素高产菌株 . 粮油食品科技 , 2017 . 25 (4 ):66 -71 . DOI:10.16210/j.cnki.1007-7561.2017.04.014http://doi.org/10.16210/j.cnki.1007-7561.2017.04.014 .

Haixia WANG , Yuan CHEN , Chao WANG , 等 . Breeding of spinosad high-producing strains by combinatorial antibiotic resistance . Science and Technology of Cereals, Oils and Foods , 2017 . 25 (4 ):66 -71 . DOI:10.16210/j.cnki.1007-7561.2017.04.014http://doi.org/10.16210/j.cnki.1007-7561.2017.04.014 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Breeding of tacrolimus high-producing strains by gamma-ray radiation and nitrosoguanidine mutagent

Related Author

No data

Related Institution

Quanzhou Medical College

⁰