Protective effect of cimetidine on immune function of mice irradiated by gamma-rays

Ying HE; Junling ZHANG; Yining ZHAO; Dingwen JIANG; Qingrong WANG; Kexian LI; Qun LUO; Xianrong SHEN

doi:10.11889/j.1000-3436.2018.rrj.36.020203

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Protective effect of cimetidine on immune function of mice irradiated by gamma-rays

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- Protective effect of cimetidine on immune function of mice irradiated by gamma-rays
- Journal of Radiation Research and Radiation Processing Vol. 36, Issue 2, (2018)
- 作者机构：
  
  1.第二军医大学海军医学研究所上海 200433
- 作者简介：
- 基金信息：
  
  Ministry Special Drugs Project of National Major Scientific and Technological Special Project for Significant New Drugs Development(2014ZX09J14103-07B)
- DOI：10.11889/j.1000-3436.2018.rrj.36.020203
  CLC：
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Ying HE, Junling ZHANG, Yining ZHAO, et al. Protective effect of cimetidine on immune function of mice irradiated by gamma-rays. [J]. Journal of Radiation Research and Radiation Processing 36(2):020203-7(2018)
DOI：

Ying HE, Junling ZHANG, Yining ZHAO, et al. Protective effect of cimetidine on immune function of mice irradiated by gamma-rays. [J]. Journal of Radiation Research and Radiation Processing 36(2):020203-7(2018) DOI： 10.11889/j.1000-3436.2018.rrj.36.020203.

摘要

雄性C57小鼠60只平均分为6组：正常对照组（0 Gy）、模型对照组（1.0 Gy）、阳性药对照组（1.0 Gy+523片）、西咪替丁（Cimetidine，CMD）低、中、高剂量组（1.0 Gy+33.3、1.0 Gy+100、1.0 Gy+300 mg/kg），采用,60,Co γ射线全身照射至吸收剂量1.0 Gy，剂量率为8.33 mGy/min。CMD于照射前连续灌胃给药6 d，每天1次，照射后5 h给药1次。照射后24 h检测脾脏淋巴细胞凋亡率、T淋巴细胞亚群、B淋巴细胞数和血清中IL-2、IL-4、IL-6、IL-12、IFN-γ和TNF-α含量。结果表明：与正常对照组比较，照射后24 h，小鼠脾脏淋巴细胞凋亡率显著增加（,p,<, 0.01），CD3,+,、CD3,+,CD8,+,T细胞和CD3,-,CD19,+,B细胞数量均下降（,p,<, 0.01），血清中的IL-4、IL-12和TNF-α含量显著降低（,p,<, 0.01，,p,<, 0.05）；与辐照模型组比较，CMD各剂量组脾脏淋巴细胞凋亡率显著下降（,p,<, 0.01），CMD给药组血清中IL-4、IL-6、IL-12、TNF-α和IFN-γ含量显著增加（,p,<, 0.01，,p,<, 0.05），CMD高剂量组的CD3,+,、CD3,+,CD4,+,和CD3,+,CD8,+,T细胞及CD3,-,CD19,+,B细胞数量均显著上升（,p,<, 0.01，,p,<, 0.05）。结果提示，CMD可有效改善γ射线辐照小鼠的免疫功能，具有较好的辐射保护作用。

Abstract

Sixty male mice were divided into six groups:normal control group (0 Gy), model control group (1.0 Gy), positive control group (1.0 Gy+523 tablet), and cimetidine groups (1.0 Gy) at the doses of 33.3, 100, and 300 mg/kg, respectively. The mice were irradiated to absorbed dose of 1.0 Gy with ,60,Co γ-rays at 8.33 mGy/min. Cimetidine was administered intragastrically 6 d before irradiation and 5 h after irradiation separately. Twenty-four hours after irradiation, the apoptosis rate of spleen lymphocytes, T lymphocyte subsets, and B lymphocytes in spleen, the content of IL-2, IL-4, IL-6, IL-12, IFN-γ, and TNF-α in serum were detected. Compared with normal control group, the apoptosis rate of spleen lymphocytes increased significantly (,p,<, 0.01). The percentage of CD3,+, CD3,+,CD8,+, T lymphocytes, CD3,−,CD19,+, B lymphocytes, and the content of IL-4, IL-12, and TNF-α in serum decreased significantly (,p,<, 0.01,p,<, 0.05). Compared with model control group, the apoptosis rate of spleen lymphocytes decreased (,p,<, 0.01), while the content of IL-4, IL-6, IL-12, IFN-γ, and TNF-α increased in serum of cimetidine groups (,p,<, 0.01,p,<, 0.05). The percentage of CD3,+, CD3,+,CD4,+, CD3,+,CD8,+, and CD3,−,CD19,+, cells increased in 300 mg/kg cimetidine group (,p,<, 0.01,p,<, 0.05). Cimetidine could improve the immune functions of mice irradiated by γ-rays to 1.0 Gy.

关键词

西咪替丁低剂量率电离辐射免疫功能细胞凋亡

Keywords

CimetidineLow-dose-rate radiationImmune functionApoptosis

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