Protective effect of glycyrrhizin magnesium (Gly-Mg) on radiation injury in mice

Gan'en MU; Yiyue JIA; Yuanyuan LI; Xiu SHEN; Zewei ZHOU

doi:10.11889/j.1000-3436.2018.rrj.36.030201

您当前的位置：

首页 >

文章列表页 >

Protective effect of glycyrrhizin magnesium (Gly-Mg) on radiation injury in mice

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- Protective effect of glycyrrhizin magnesium (Gly-Mg) on radiation injury in mice
- Journal of Radiation Research and Radiation Processing Vol. 36, Issue 3, (2018)
- 作者机构：
  
  1.中国医学科学院北京协和医学院放射医学研究所天津 300192
- 作者简介：
- 基金信息：
  
  Tianjin Application Foundation and Research in Cutting-edge Technologies Program(15JCZDJC33400)
- DOI：10.11889/j.1000-3436.2018.rrj.36.030201
  CLC：
扫描看全文
Gan'en MU, Yiyue JIA, Yuanyuan LI, et al. Protective effect of glycyrrhizin magnesium (Gly-Mg) on radiation injury in mice. [J]. Journal of Radiation Research and Radiation Processing 36(3):030201-7(2018)
DOI：

Gan'en MU, Yiyue JIA, Yuanyuan LI, et al. Protective effect of glycyrrhizin magnesium (Gly-Mg) on radiation injury in mice. [J]. Journal of Radiation Research and Radiation Processing 36(3):030201-7(2018) DOI： 10.11889/j.1000-3436.2018.rrj.36.030201.

摘要

考察甘草甜素镁（Glycyrrhizin magnesium，Gly-Mg）的抗氧化活性及其对DNA损伤的防护作用。将小鼠分为空白对照组（生理盐水）、照射对照组（抗氧化实验：生理盐水+6.0 Gy；彗星实验：生理盐水+7.2 Gy）和照射给药组（抗氧化实验：50 mg/kg Gly-Mg+6.0 Gy、75 mg/kg Gly-Mg+6.0 Gy；彗星实验：50 mg/kg Gly-Mg+7.2 Gy、75 mg/kg Gly-Mg+7.2 Gy）。采用抗氧化试剂盒检测小鼠血清、肝组织和肺组织中超氧化物歧化酶（Superoxide dismutase，SOD）、过氧化氢酶（Catalase，CAT）的活性和谷胱甘肽（Glutathione，GSH）、脂质过氧化物丙二醛（Methylene dioxyamphetamine，MDA）的含量。结果表明：Gly-Mg能够提高小鼠血清和组织中SOD、CAT的活性；增加抗氧化低分子清除剂GSH含量，并降低MDA的含量。彗星实验结果表明，与照射对照组相比，照射给药组小鼠的彗星尾长、彗星尾矩、Olive尾矩、尾部DNA含量和尾部DNA含量百分比均显著降低（,p,<, 0.01）。实验结果提示，目标化合物Gly-Mg对辐照导致的氧化损伤有一定的防护作用，而对小鼠外周血淋巴细胞的DNA损伤则具有显著的防护作用。

Abstract

This study utilized antioxidant and basic single cell gel electrophoresis experiments to assess the effect of glycyrrhizin magnesium (Gly-Mg) on oxidative and DNA damage. ICR (Institute of Cancer Research) mice were divided into a control group (normal saline), irradiation control group (antioxidant experiment:normal saline+6.0 Gy; comet test:normal saline+7.2 Gy), and Gly-Mg group (antioxidant experiment:50 mg/kg+6.0 Gy, 75 mg/kg+6.0 Gy; comet test:50 mg/kg+7.2 Gy, 75 mg/kg+7.2 Gy). The activity of superoxide dismutase (SOD) and catalase (CAT), and the content of glutathione (GSH) and methylene dioxyamphetamine (MDA) in the serum, liver, and lung tissues of mice were detected using antioxidant kits. Results of the oxidative damage protection tests suggested that Gly-Mg can improve the activities of SOD and CAT, increase the content of GSH, and reduce the content of MDA in mouse serum and tissues. Meanwhile, results of the comet test showed that Gly-Mg groups had a significant protective effect on radiation-induced DNA damage in bone marrow compared with that in the irradiation control group (,p,<, 0.01). In conclusion, Gly-Mg has protective effect against antioxidant and DNA damage.

关键词

甘草甜素镁辐射防护抗氧化彗星实验

Keywords

Glycyrrhizin magnesium (Gly-Mg)RadioprotectionAntioxidationComet assay

references

Z H Zhu , W W Tao , J P Li , 等 . Rapid determination of flavonoids in licorice and comparison of three licorice species . Journal of Separation Science , 2016 . 39 (3 ):473 -482 . DOI:10.1002/jssc.201500685http://doi.org/10.1002/jssc.201500685 .

R Yang , B C Yuan , Y S Ma , 等 . The anti-inflammatory activity of licorice, a widely used Chinese herb . Pharmaceutical Biology , 2017 . 55 (1 ):5 -18 . DOI:10.1080/13880209.2016.1225775http://doi.org/10.1080/13880209.2016.1225775 .

张胜 . 《伤寒论》中甘草用义浅探 . 中医药研究 , 2001 . 17 (1 ):6 -7. .

Sheng ZHANG . The use of glycyrrhiza in 《Treatise on febrile diseases》 . Research of Traditional Chinese Medicine , 2001 . 17 (1 ):6 -7. .

张明发 , 沈雅琴 . 甘草酸及其苷元甘草次酸的糖皮质激素样作用 . 现代药物与临床 , 2011 . 26 (1 ):33 -35 . http://mall.cnki.net/magazine/Article/GWZW201101007.htmhttp://mall.cnki.net/magazine/Article/GWZW201101007.htm, .

Mingfa ZHANG , Yaqin SHEN . Glucocorticoid-like effect of glycyrrhizic acid and its aglycone glycyrrhetic acid . Drugs & Clinic , 2011 . 26 (1 ):33 -35 . http://mall.cnki.net/magazine/Article/GWZW201101007.htmhttp://mall.cnki.net/magazine/Article/GWZW201101007.htm, .

王肖娜 , 金玉姬 , 刘洋 , 等 . 甘草甜素的应用及研究现状 . 吉林医药学院学报 , 2014 . 35 (2 ):144 -147 . DOI:10.13845/j.cnki.issn1673-2995.2014.02.022http://doi.org/10.13845/j.cnki.issn1673-2995.2014.02.022 .

Xiaona WANG , Yuji JIN , Yang LIU , 等 . Application and research of glycyrrhizin . Journal of Jilin Medical College , 2014 . 35 (2 ):144 -147 . DOI:10.13845/j.cnki.issn1673-2995.2014.02.022.issn1673-2995.2014.02.022http://doi.org/10.13845/j.cnki.issn1673-2995.2014.02.022.issn1673-2995.2014.02.022 .

T Y Wu , T O Khor , C L Saw , 等 . Anti-inflammatory/anti-oxidative stress activities and differential regulation of Nrf2-mediated genes by non-polar fractions of tea chrysanthemum zawadskii and licorice glycyrrhiza uralensis . American Association of Pharmaceutical Scientists , 2011 . 13 (1 ):1 -13 . DOI:10.1208/s12248-010-9239-4http://doi.org/10.1208/s12248-010-9239-4 .

M A Takhshid , M Davood , A Jafar , 等 . The healing effect of licorice extract in acetic acid-induced ulcerative colitis in rat model . Comparative Clinical Pathology , 2012 . 21 (6 ):1139 -1144 . DOI:10.1007/s00580-011-1249-9http://doi.org/10.1007/s00580-011-1249-9 .

E I Azzam , J P Jay-Gerin , D Pain . Ionizing radiation-induced metabolic oxidative stress and prolonged cell injury . Cancer Letters , 2012 . 327 (1-2 ):48 -60 . DOI:10.1016/j.canlet.2011.12.012http://doi.org/10.1016/j.canlet.2011.12.012 .

W W Kam , R B Banati . Effects of ionizing radiation on mitochondria . FreeRadicalBiology and Medicine , 2013 . 65 607 -619 . DOI:10.1016/j.freeradbiomed.2013.07.024http://doi.org/10.1016/j.freeradbiomed.2013.07.024 .

I Szumiel . Ionizing radiation-induced oxidative stress, epigenetic changes and genomic instability:the pivotal role of mitochondria . International Journal of Radiation Biology , 2015 . 91 (1 ):1 -12 . DOI:10.3109/09553002.2014.934929http://doi.org/10.3109/09553002.2014.934929 .

龙伟 , 牟感恩 , 张广慧 , 等 . 黑茶提取物的辐射防护作用及其机制研究 . 国际放射核医学杂志 , 2017 . 41 (6 ):407 -412 . DOI:10.3760/cma.j.issn.1673-4114.2017.06.006http://doi.org/10.3760/cma.j.issn.1673-4114.2017.06.006 .

Wei LONG , Gan'en MU , Guanghui ZHANG , 等 . Anti-radiation effects of dark tea and its mechanism . International Journal of Radiation Medicine Nuclear Medicine , 2017 . 41 (6 ):407 -412 . DOI:10.3760/cma.j.issn.1673-4114.2017.06.006http://doi.org/10.3760/cma.j.issn.1673-4114.2017.06.006 .

郑啓盛 , 于光允 , 贺欣 , 等 . S-异戊烯基-L-半胱氨酸对辐射诱导小鼠DNA损伤的保护作用 . 中国医学科学院学报 , 2015 . 37 (5 ):496 -500 . DOI:10.3881/j.issn.1000-503X.2015.05.002http://doi.org/10.3881/j.issn.1000-503X.2015.05.002 .

Qisheng ZHENG , Guangyun YU , Xin HE , 等 . Protective effect of S-isopentenyl-L-cysteine against DNA damage in irradiated mice . Acta Acadeniae Medicinae Sinicae , 2015 . 37 (5 ):496 -500 . DOI:10.3881/j.issn.1000-503X.2015.05.002http://doi.org/10.3881/j.issn.1000-503X.2015.05.002 .

牟感恩 , 龙伟 , 李园园 , 等 . N-乙酰基-S-烯丙基-L-半胱氨酸的合成及其抗辐射活性 . 辐射研究与辐射工艺学报 , 2017 . 35 (5 ):050201 DOI:10.11889/j.1000-3436.2017.rrj.35.050201http://doi.org/10.11889/j.1000-3436.2017.rrj.35.050201 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract431.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract431.shtml, .

Gan'en MU , Wei LONG , Yuanyuan LI , 等 . Synthesis and anti-irradiation activities of N-acetyl-S-allyl-L-cysteine . Journal of Radiation Research and Radiation Processing , 2017 . 35 (5 ):050201 DOI:10.11889/j.1000-3436.2017.rrj.35.050201http://doi.org/10.11889/j.1000-3436.2017.rrj.35.050201 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract431.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract431.shtml, .

J R Milligan , J A Aguilera , R A Paglinawan , 等 . DNA strand break yields after post-high LET irradiation incubation with end on uclease-Ⅲ and evidence for hydroxyl radical clustering . International Journal of Radiation Biology , 2001 . 77 (2 ):155 -64 . DOI:10.1080/09553000010013445http://doi.org/10.1080/09553000010013445.

M Reyazuddin , S A Azmi , N Islam , 等 . Oxidative stress and level of antioxidant enzymes in drug-naive schizophrenics . Indian Journal of Psychiatry , 2014 . 56 (4 ):344 -349 . DOI:10.4103/0019-5545.146516http://doi.org/10.4103/0019-5545.146516 .

H Deng , W Shen , Y Peng , 等 . Nanoparticulate peroxidase/catalase mimetic and its application . Chemistry , 2012 . 18 (29 ):8906 -8911 . DOI:10.1002/chem.201200643http://doi.org/10.1002/chem.201200643 .

X Su , Y He , W Yang , 等 . Effect of Dan Hong injection on PON1, SOD activity and MDA levels in elderly patients with coronary heart disease . International Journal of Clinical Experimental Medicine , 2014 . 7 (12 ):5886 -5889. .

S M Kumar , K Swaminathan , D L Clemens , 等 . GSH protects against oxidative stress and toxicity in VL-17A cells exposed to high glucose . European Journal of Nutrition , 2015 . 54 (2 ):223 -234 . DOI:10.1007/s00394-014-0703-2http://doi.org/10.1007/s00394-014-0703-2 .

P Jackson , L M Pedersen , Z O Kyjovska , 等 . Validation of freezing tissues and cells for analysis of DNA strand break levels by comet assay . Mutagenesis , 2013 . 28 (6 ):699 -707 . DOI:10.1093/mutage/get049http://doi.org/10.1093/mutage/get049 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

DNA damages in Tillandsia species caused by the radioactive gas radon

Protective effect of N-oxalyl-D-phenylalanine on radiation injury in mice

Radioprotective effect of beta-D-glucan derived from Saccharomyces cerevisiae on human umbilical vein endothelial cells

Reasonable threshold value used to segment the individual comet from the comet assay image

The radioprotection of fullerenols against the stylonychia mytilus exposed to γ-rays

Related Author

No data

Related Institution

School of Resources and Environment, Qingdao Agricultural University

School of Food Science, Nanjing Xiaozhuang University

Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College

Institute of Modern Physics, Chinese Academy of Sciences

University of Chinese Academy of Sciences

⁰