Effects of electromagnetic pulse exposure on development of testicle in the growing male mice

Yanyun LIN; Yafeng WANG; Peng GAO; Qiyan GUO; Junfeng HU; Junye LIU; Guozhen GUO; Haiyang LANG; Lihua ZENG

doi:10.11889/j.1000-3436.2018.rrj.36.040202

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Effects of electromagnetic pulse exposure on development of testicle in the growing male mice

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- Effects of electromagnetic pulse exposure on development of testicle in the growing male mice
- Journal of Radiation Research and Radiation Processing Vol. 36, Issue 4, (2018)
- 作者机构：
  
  1.空军军医大学军事预防医学系放射医学教研室西安 710032
  2.海军总医院海军卫生防疫队北京 101113
- 作者简介：
- 基金信息：
  
  National Natural Science Foundation of China(51677189);National Natural Science Foundation of China(51437008);Natural Science Foundation of Shaanxi Province(2014KTCQ03-02)
- DOI：10.11889/j.1000-3436.2018.rrj.36.040202
  CLC：
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Yanyun LIN, Yafeng WANG, Peng GAO, et al. Effects of electromagnetic pulse exposure on development of testicle in the growing male mice. [J]. Journal of Radiation Research and Radiation Processing 36(4):040202-7(2018)
DOI：

Yanyun LIN, Yafeng WANG, Peng GAO, et al. Effects of electromagnetic pulse exposure on development of testicle in the growing male mice. [J]. Journal of Radiation Research and Radiation Processing 36(4):040202-7(2018) DOI： 10.11889/j.1000-3436.2018.rrj.36.040202.

摘要

将96只3周龄雄性BALB/c小鼠按体质量随机分为假暴露组和暴露组。暴露组小鼠每日接受场强200 kV/m、脉冲数400次电磁脉冲(Electromagnetic pulse, EMP)暴露，连续暴露30 d，2 h/d，暴露期间观测小鼠体质量的变化。于照后第1、7、14、21、30和60天检测暴露后小鼠睾丸指数、精子数量、精子畸形率、精子凋亡及睾丸组织中肿瘤转移相关基因-1 (Metastasis tumor antigen 1, MTA-1)蛋白和信使核糖核酸(mRNA)表达量。结果表明：与假暴露组相比，暴露组小鼠体质量从照射第8天至第28天明显降低；睾丸指数于照后第21天至第60天明显降低；精子数量于照后第7天至第60天明显减少；精子畸形率从照后第1天至第30天时增高；生精细胞凋亡率在照后第1天和第60天时明显增高，但在照后第7天和第14天时明显降低；睾丸组织中MTA-1蛋白表达量于照后第1、7、14和60天时明显降低，且MTA-1 mRNA表达量也于照射后第1天至第21天时明显降低。结果提示一定参数的EMP暴露可能通过影响MTA-1表达水平而对幼龄雄性BALB/c小鼠睾丸发育造成损伤。

Abstract

Ninety-six mice aged 3 weeks were randomly divided into two groups: a group exposed to electromagnetic pulse (EMP) at 200 kV/m in 400 pulses for 30 d, 2 h/d, and a sham-exposure group. The body weights were measured during the exposure time. The testicular index; sperm count, sperm deformity rate, spermatogenic cells apoptosis; and metastasis tumor antigen 1 (MTA-1) protein and mRNA expression in testicles were measured at 1-, 7-, 14-, 21-, 30-, and 60-day time points after exposure. The results showed that body weights in the exposure group were significantly lower than those in the sham-exposure group on days 8~28 of EMP exposure (,p,<, 0.05); compared with that in the sham-exposure group, testicular index in the exposure group decreased significantly on days 21~60 after exposure; sperm count decreased on days 7~60 after exposure; the rate of sperm deformation increased on days 1~30 after exposure; apoptotic spermatogenic cells increased on days 1 and 60 but decreased on days 7 and 14 after exposure (,p,<, 0.05); MTA-1 protein expression in testicles decreased significantly at all time points after exposure; and mRNA expression decreased on days 1~21 after exposure. The reduced testicular indexes and sperm quality, as measured by the decreases in MTA-1 protein and mRNA, suggest that EMP exposure inhibits the development of testicles in growing male mice.

关键词

电磁脉冲BALB/c小鼠睾丸精子肿瘤转移相关基因-1

Keywords

Electromagnetic pulse (EMP)BALB/c miceTesticleSpermMetastasis tumor antigen 1 (MTA-1)

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