Monitoring dopamine release from neuron-like cells

Min LI; Yueyue ZHANG; Jiangbo LIU; Lihua WANG; Qian LI; Xiaolei ZUO

doi:10.11889/j.1000-3436.2018.rrj.36.050201

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Monitoring dopamine release from neuron-like cells

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- Monitoring dopamine release from neuron-like cells
- Journal of Radiation Research and Radiation Processing Vol. 36, Issue 5, (2018)
- 作者机构：
  
  1.中国科学院上海应用物理研究所物理生物学研究室上海光源生物成像中心上海 201800
  2.中国科学院大学北京 100049
  3.上海交通大学医学院附属仁济医院上海 200127
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(21422508);National Natural Science Foundation of China(31470960);Research Equipment Development Project of the Chinese Academy of Sciences(yz201438)
- DOI：10.11889/j.1000-3436.2018.rrj.36.050201
  CLC：
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Min LI, Yueyue ZHANG, Jiangbo LIU, et al. Monitoring dopamine release from neuron-like cells. [J]. Journal of Radiation Research and Radiation Processing 36(5):050201-6(2018)
DOI：

Min LI, Yueyue ZHANG, Jiangbo LIU, et al. Monitoring dopamine release from neuron-like cells. [J]. Journal of Radiation Research and Radiation Processing 36(5):050201-6(2018) DOI： 10.11889/j.1000-3436.2018.rrj.36.050201.

摘要

采用火焰刻蚀法制备碳纤维纳米电极（CFNE），通过扫描电子显微镜对纳米电极直径进行表征，采用循环伏安法对纳米电极的电化学性能进行验证。选用差分脉冲伏安法验证了纳米电极对多巴胺（Dopamine，DA）的体外响应，最后采用膜片钳电压钳模式，施加电压700 mV（Ag/AgCl为参比），对高分化的PC12细胞施加高钾刺激，实时监测DA的释放。结果表明：CFNE的直径可低至~100 nm，电极表面存在优良的非线性扩散特性；CFNE在体外对DA有灵敏的响应，检测限达10 pmol/L；对PC12细胞高钾刺激后，实时监测到PC12细胞单个释放位点的DA释放过程。

Abstract

A carbon fiber nanoelectrode (CFNE) was prepared using the flame-etching method. Scanning electron microscopy and cyclic voltammetry were used to characterize its diameter and the electrochemical properties, respectively. Differential pulse voltammetry was used to detect dopamine (DA) ,in vitro,. A patch clamp in the voltage-clamp mode was used to monitor the release of DA from PC12 cells after stimulation with an elevated K,+, solution, maintaining the voltage at 700 mV. The results showed that CFNE was successfully prepared with a diameter of ~100 nm, and excellent nonlinear diffusion behavior was observed on the nanoelectrode surface. Further, the nanoelectrode showed excellent response to DA with the limit of detection of 10 pmol/L. After PC12 cells were stimulated with the elevated K,+, solution, the DA release process of PC12 cells at a single release site was monitored in real time.

关键词

碳纤维纳米电极类神经细胞单释放位点多巴胺实时监测

Keywords

Carbon fiber nanoelectrodeNeuron-like cellSingle release siteDopamineReal-time monitoring

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