Effects of gamma-ray irradiation on cognitive function and neuronal death in mice

Aitao NAI; Zhen WANG; Liyun TANG; Wei WAN; Zhenghai LIU; Yang XU; Xiaohong AI; Shuya HE; Wenyu CAO

doi:10.11889/j.1000-3436.2018.rrj.36.050202

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Effects of gamma-ray irradiation on cognitive function and neuronal death in mice

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- Effects of gamma-ray irradiation on cognitive function and neuronal death in mice
- Journal of Radiation Research and Radiation Processing Vol. 36, Issue 5, (2018)
- 作者机构：
  
  1.南华大学附属第一医院肿瘤放疗科衡阳 421001
  2.南华大学医学院应用解剖与生殖医学研究所衡阳 421001
  3.南华大学医学院生理教研室衡阳 421001
  4.南华大学公共卫生学院放射医学教研室衡阳 421001
- 作者简介：
- 基金信息：
  
  the Natural Science Foundation of Hunan Province of China(2018JJ3460);the Hunan Provincial Natural Science Fund Provincial and Municipal Joint Fund Project(2018JJ4034);the Education Department Foundation of Hunan Province of China(14A125);the Hunan Provincial Education Department Foundation of China(17B230);The Start-up Funds for PhDs in University of South China(2015XQD27);The Start-up Funds for PhDs in University of South China(2015XQD28);the Scientific Research Innovation Project for Postgraduate student of Hunan Province in 2018
- DOI：10.11889/j.1000-3436.2018.rrj.36.050202
  CLC：
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Aitao NAI, Zhen WANG, Liyun TANG, et al. Effects of gamma-ray irradiation on cognitive function and neuronal death in mice. [J]. Journal of Radiation Research and Radiation Processing 36(5):050202-6(2018)
DOI：

Aitao NAI, Zhen WANG, Liyun TANG, et al. Effects of gamma-ray irradiation on cognitive function and neuronal death in mice. [J]. Journal of Radiation Research and Radiation Processing 36(5):050202-6(2018) DOI： 10.11889/j.1000-3436.2018.rrj.36.050202.

摘要

57只2月龄雌性昆明小鼠分别接受不同剂量,137,Cs γ射线（0、4、6、8、10 Gy）单次全身照射，观察小鼠生存率及体重变化情况，35 d后进行旷场实验、新旧位置识别实验行为学检测，并采用Western blot方法检测小鼠海马区神经元标记物NeuN的表达。结果表明：4 Gy剂量组小鼠生存率100%，且一般健康状况较好，而给予8 Gy及10 Gy吸收剂量时，小鼠死亡率明显增加；与对照组相比，4 Gy及6 Gy剂量组小鼠在新旧位置识别实验中，对新位置的时间辨别指数百分比均明显降低（36.04%±4.39% ,vs,. 11.45%±6.34% ,vs,. -1.40%±9.27%，,p,<, 0.05）；海马区NeuN蛋白表达均明显下调（1.000 ±0.045 ,vs,. 0.795 ±0.052 ,vs,. 0.332 ±0.024，,p,<, 0.05），且6 Gy剂量组下调趋势更加明显。研究表明，在一定剂量范围内（6 Gy及以下吸收剂量），,137,Cs γ射线全身照射诱导小鼠认知功能障碍及神经元损害随着吸收剂量的增加而越发明显。

Abstract

Fifty-seven female Kunming mice aged 2 months received full-body irradiation once with ,137,Cs γ-rays at doses of 0, 4, 6, 8, and 10 Gy. The survival rate and body weight were then observed for 35 d. The animals' behaviors were analyzed using open-field and novelty location tests 35 d post irradiation. The expression of the neuronal marker NeuN in hippocampus was determined using western blot. The results showed that the mice in the 4 Gy group exhibited a higher survival rate and superior general status than did those in the other groups. Compared with the control group, the mice in the 4 and 6 Gy groups had a lower discrimination index in the novelty location test (36.04%±4.39% ,vs,. 11.45%±6.34% ,vs,. -1.40%±9.27%, respectively,p,<, 0.05), and had a remarkably lower level of NeuN expression in hippocampus (1.000±0.045 ,vs,. 0.795±0.052 ,vs,. 0.332±0.024, respectively,p,<, 0.05). Meanwhile, the mice in the 6 Gy group exhibited more serious cognitive dysfunction and neuronal death than did those in the 4 Gy group. The mice in the 8 and 10 Gy groups showed a higher death rate than those in the 4 and 6 Gy groups. However, no significant difference in survival rate was found between the mice in the 4 and 6 Gy groups. Our results suggest that ,137,Cs γ-ray irradiation can induce cognitive dysfunction and neuronal death in a dose-dependent manner at a dose of ,<, 6 Gy.

关键词

电离辐射γ射线认知功能神经元

Keywords

Ionizing radiationγ-raysCognitive functionNeuron

references

D Li , P Kong , J Sun , 等 . Comparison of total-body irradiation before and after chemotherapy in pretreatment for hematopoietic stem cell transplantation . Cancer Biother Radiopharm , 2012 . 27 (2 ):119 -123 . DOI:10.1089/cbr.2011.1041http://doi.org/10.1089/cbr.2011.1041 .

J M Encinas , M E Vazquez , R C Switzer , 等 . Quiescent adult neural stem cells are exceptionally sensitive to cosmic radiation . Experimental Neurology , 2008 . 210 (1 ):274 -279 . DOI:10.1016/j.expneurol.2007.10.021http://doi.org/10.1016/j.expneurol.2007.10.021 .

孔雪源 , 刘玉龙 , 张冰洁 , 等 . 某市放射工作人员焦虑抑郁状况及影响因素分析 . 中华放射医学与防护杂志 , 2014 . 34 (5 ):367 -370 . DOI:10.3760/cma.j.issn.0254-5098.2014.05.013http://doi.org/10.3760/cma.j.issn.0254-5098.2014.05.013 .

Xueyuan KONG , Yulong LIU , Bingjie ZHANG , 等 . Logistic analysis on influencing factors of radiation workers anxiety and depression in some city . Chinese Journal of Radiation Medicine and Protection , 2014 . 34 (5 ):367 -370 . DOI:10.3760/cma.j.issn.0254-5098.2014.05.013http://doi.org/10.3760/cma.j.issn.0254-5098.2014.05.013 .

X R Lowe , S Bhattacharya , F Marchetti , 等 . Early brain response to low-dose radiation exposure involves molecular networks and pathways associated with cognitive functions, advanced aging and Alzheimer's disease . Radiation Research , 2009 . 171 (1 ):53 -65 . DOI:10.1667/RR1389.1http://doi.org/10.1667/RR1389.1 .

孙锐 , 张力元 , 冀胜军 , 等 . 10 cGy单次全脑照射引起大鼠认知功能损害的初步实验研究 . 中华放射肿瘤学杂志 , 2012 . 21 (5 ):474 -476 . DOI:10.3760/cma.j.issn.1004-4221.2012.05.023http://doi.org/10.3760/cma.j.issn.1004-4221.2012.05.023 .

Rui SUN , Liyuan ZHANG , Shengjun JI , 等 . An experimental study of 10 cGy whole brain radiation induced cognitive dysfunction . Chinese Journal of Radiation Oncology , 2012 . 21 (5 ):474 -476 . DOI:10.3760/cma.j.issn.1004-4221.2012.05.023http://doi.org/10.3760/cma.j.issn.1004-4221.2012.05.023 .

胡雅梦 , 刘琅嬛 , 刘宝婵 , 等 . 黄芪甲苷对肝细胞的辐射防护作用 . 辐射研究与辐射工艺学报 , 2015 . 33 (6 ):060202 DOI:10.11889/j.1000-3436.2015.rrj.33.060202http://doi.org/10.11889/j.1000-3436.2015.rrj.33.060202 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract283.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract283.shtml, .

Yameng HU , Langhuan LIU , Baochan LIU , 等 . Radioprotective effects of astragaloside Ⅳ on the liver cell . Journal of Radiation Research and Radiation Processing , 2015 . 33 (6 ):060202 DOI:10.11889/j.1000-3436.2015.rrj.33.060202http://doi.org/10.11889/j.1000-3436.2015.rrj.33.060202 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract283.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract283.shtml, .

R D Rosa , A A Garcia , C Braschi , 等 . Intranasal administration of nerve growth factor (NGF) rescues recognition memory deficits in AD11 anti-NGF transgenic mice . Proceedings of the National Academy of Sciences of the United States of America , 2005 . 102 (10 ):3811 -3816 . DOI:10.1073/PNAS.0500195102http://doi.org/10.1073/PNAS.0500195102 .

W Cao , J Duan , X Wang , 等 . Early enriched environment induces an increased conversion of proBDNF to BDNF in the adult rat's hippocampus . Behavioural Brain Research , 2014 . 265 76 -83 . DOI:10.1016/j.bbr.2014.02.022http://doi.org/10.1016/j.bbr.2014.02.022 .

林素兰 , 丁楠 , 危文俊 , 等 . GANRA纳米药对淋巴母细胞的辐射防护作用及辐射敏感microRNA的影响 . 辐射研究与辐射工艺学报 , 2017 . 35 (3 ):030201 DOI:10.118879/j.1000-3436.2017.rrj.35.030201http://doi.org/10.118879/j.1000-3436.2017.rrj.35.030201 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract395.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract395.shtml, .

Sulan LIN , Nan DING , Wenjun WEI , 等 . Assessment of the protective effects of GANRA nanoparticles against X-ray radiation-induced cellular damage and microRNA alterations . Journal of Radiation Research and Radiation Processing , 2017 . 35 (3 ):030201 DOI:10.118879/j.1000-3436.2017.rrj.35.030201http://doi.org/10.118879/j.1000-3436.2017.rrj.35.030201 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract395.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract395.shtml, .

L Wei , B J Leibowitz , X Wang , 等 . Inhibition of CDK4/6 protects against radiation-induced intestinal injury in mice . Journal of Clinical Investigation , 2016 . 126 (11 ):4076 -4087 . DOI:10.1172/JCI88410http://doi.org/10.1172/JCI88410 .

J Dietrich , N Baryawno , N Nayyar , 等 . Bone marrow drives central nervous system regeneration after radiation injury . The Journal of Clinical Investigation , 2018 . 128 (1 ):281 -293 . DOI:10.1172/JCI90647http://doi.org/10.1172/JCI90647 .

夏欣欣 , 李跃军 , 周冬枝 , 等 . 二七方对急性放射性肠损伤大鼠肠道的保护作用及机制 . 广州中医药大学学报 , 2017 . 34 (4 ):559 -565 . DOI:10.13359/j.cnki.gzxbtcm.2017.04.021http://doi.org/10.13359/j.cnki.gzxbtcm.2017.04.021 .

Xinxin XIA , Yuejun LI , Dongzhi ZHOU , 等 . Protective effect of Erqi Decoation on intestinal tract of acute radiation intestinal injury rats and its mechanism . Journal of Guangzhou University of Traditional Chinese Medicine , 2017 . 34 (4 ):559 -565 . DOI:10.13359/j.cnki.gzxbtcm.2017.04.021http://doi.org/10.13359/j.cnki.gzxbtcm.2017.04.021 .

牟感恩 , 龙伟 , 李园园 , 等 . N-乙酰基-S-烯丙基-L-半胱氨酸的合成及其抗辐射活性 . 辐射研究与辐射工艺学报 , 2017 . 35 (5 ):050201 DOI:10.11889/j.1000-3436.2017.rrj.35.050201http://doi.org/10.11889/j.1000-3436.2017.rrj.35.050201 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract431.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract431.shtml, .

Gan'en MU , Wei LONG , Yuanyuan LI , 等 . Synthesis and anti-irradiation activities of N-acetyl-S-allyl-L-cysteine . Journal of Radiation Research and Radiation Processing , 2017 . 35 (5 ):050201 DOI:10.11889/j.1000-3436.2017.rrj.35.050201http://doi.org/10.11889/j.1000-3436.2017.rrj.35.050201 http://www.j.sinap.ac.cn/fushe/CN/abstract/abstract431.shtmlhttp://www.j.sinap.ac.cn/fushe/CN/abstract/abstract431.shtml, .

Y Xu , W Cao , M Zhou , 等 . Inactivation of BRD7 results in impaired cognitive behavior and reduced synaptic plasticity of the medial prefrontal cortex . Behavioural Brain Research , 2015 . 286 1 -10 . DOI:10.1016/j.bbr.2015.02.031http://doi.org/10.1016/j.bbr.2015.02.031 .

T M Madsen , P E Kristjansen , T G Bolwig , 等 . Arrested neuronal proliferation and impaired hippocampal function following fractionated brain irradiation in the adult rat . Neuroscience , 2003 . 119 (3 ):635 -642 . DOI:10.1016/S0306-4522(03)00199-4http://doi.org/10.1016/S0306-4522(03)00199-4 .

L A Christie , M M Acharya , C L Limoli . Quantifying cognitive decrements caused by cranial radiotherapy . Journal of Visualized Experiments , 2011 . (56 ):e3108 DOI:10.3791/3108http://doi.org/10.3791/3108 .

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