Environmental enrichment improves irradiation-induced cognitive dysfunction

Liyun TANG; Aitao NAI; Zhen WANG; Yang XU; Zhenghai LIU; Jie HE; Xi CHEN; Shuya HE; Xiaolin ZHONG; Yinjuan TANG; Wei WAN; Xiaohong AI; Wenyu CAO

doi:10.11889/j.1000-3436.2018.rrj.36.060201

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Environmental enrichment improves irradiation-induced cognitive dysfunction

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2021-12-10

- Environmental enrichment improves irradiation-induced cognitive dysfunction
- Journal of Radiation Research and Radiation Processing Vol. 36, Issue 6, (2018)
- 作者机构：
  
  1.南华大学附属第一医院肿瘤放疗科衡阳 421001
  2.南华大学医学院应用解剖与生殖医学研究所衡阳 421001
  3.南华大学医学院生理教研室衡阳 421001
  4.南华大学医学院病理教研室衡阳 421001
  5.南华大学公共卫生学院放射医学教研室衡阳 421001
  6.南华大学附属第一医院临床医学研究所衡阳 421001
  7.湘南学院基础医学院郴州 423000
- 作者简介：
- 基金信息：
  
  the Natural Science Foundation of Hunan Province of China(2018JJ3460);the Natural Science Foundation of Hunan Province of China(2018JJ3429);the Natural Science Foundation of Hunan Province of China(2018JJ3464);the Hunan Provincial Natural Science Fund Provincial and Municipal Joint Fund Project(2018JJ4034);the Education Department Foundation of Hunan Province of China(14A125);the Education Department Foundation of Hunan Province of China(16A187);the Education Department Foundation of Hunan Province of China(16B245);the Hunan Provincial Education Department Foundation of China(17B230);Planning Project of Hunan Provincial Health and Family Planning Commission Research Projects(C2016042);The Start-up Funds for PhDs in University of South China(2015XQD27);The Start-up Funds for PhDs in University of South China(2015XQD28);Applied Anatomy and Reproductive Medicine Key Laboratory Construction Project in Hengyang(2017KJ182);the Science Foundation Project for Postgraduate student of the University of South China in 2018(2018KYZ065);the Science Foundation Project for Postgraduate student of the University of South China in 2018(2018KYZ069)
- DOI：10.11889/j.1000-3436.2018.rrj.36.060201
  CLC：
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Liyun TANG, Aitao NAI, Zhen WANG, et al. Environmental enrichment improves irradiation-induced cognitive dysfunction. [J]. Journal of Radiation Research and Radiation Processing 36(6):060201-7(2018)
DOI：

Liyun TANG, Aitao NAI, Zhen WANG, et al. Environmental enrichment improves irradiation-induced cognitive dysfunction. [J]. Journal of Radiation Research and Radiation Processing 36(6):060201-7(2018) DOI： 10.11889/j.1000-3436.2018.rrj.36.060201.

摘要

探讨丰富环境（Environmental Enrichment，EE）对电离辐射所致小鼠认知功能障碍的保护作用及其可能机制。将36只雌性昆明小鼠随机分为对照组（Control）、辐射组（IR）和辐射丰富环境组（IR+EE）。IR组和IR+EE组小鼠采用,137,Cs γ射线进行全身辐照至吸收剂量4 Gy；IR+EE组小鼠辐照后给予连续35 d EE刺激。采用新物体识别实验检测小鼠认知功能；免疫组织化学方法检测海马区神经发生标记物双皮质素（Doublecortin，DCX）及磷酸化环磷酸腺苷应答元件结合蛋白（Phosphorylated cyclic adenosine monophosphate（cAMP）response element-binding protein，p-CREB）的表达；Western blot方法检测海马区CREB及p-CREB蛋白的表达。结果表明：与对照组相比，IR组小鼠新物体分辨率明显降低（45.55±5.80 ,vs,. 2.99±6.18，,p,<, 0.05），海马区DCX阳性细胞数明显减少（123.8±9.3 ,vs,. 70.2±5.9，,p,<, 0.05），p-CREB/CREB表达下调（1.007±0.058 ,vs,.0.772±0.039，,p,<, 0.05）；而予以EE刺激后小鼠新物体分辨率明显回升（2.99±6.18 ,vs,. 28.31±7.30，,p,<, 0.05），海马区DCX阳性细胞数明显增加（70.2±5.9 ,vs,. 95.7±6.5，,p,<, 0.05），p-CREB/CREB表达上调（0.772±0.039 ,vs,. 1.014±0.093，,p,<, 0.05）。结果提示EE可能是通过上调海马区p-CREB的表达，保护海马神经发生，进而改善辐射所致的小鼠认知功能障碍。

Abstract

To investigate the protective effect and potential mechanism of environmental enrichment (EE) on irradiation-induced cognitive dysfunction in mice, thirty-six female Kunming mice were randomly divided into three groups administered different treatments:control, Irradiation (IR), and IR+EE. The mice in IR group and IR+EE group were irradiated with ,137,Cs g rays at an absorbed dose of 4.0 Gy. Mice in the IR+EE group were housed in EE for 35 d following irradiation. The object recognition task was used to evaluate the cognitive function of mice. The expression of the neurogenesis marker doublecortin (DCX), and phosphorylated cAMP response element-binding protein (p-CREB) was detected by using immunohistochemical staining. The expression of the cAMP response element-binding protein (CREB) and p-CREB proteins in hippocampus was assayed by using western blotting. The results showed that compared with control group, the irradiation group had a low discrimination ratio in the object recognition task (45.55±5.80 ,vs,. 2.99±6.18,p,<, 0.05), had fewer DCX positive cells (123.8±9.3 ,vs,. 70.2±5.9,p,<, 0.05), and had a remarkably lower level of p-CREB/CREB (1.007±0.058 ,vs,. 0.772±0.039,p,<, 0.05). Compared with the IR group, the IR+EE group had an enhanced discrimination ratio (2.99±6.18 ,vs,. 28.31±7.30,p,<, 0.05), more DCX positive cells (70.2±5.9 ,vs,. 95.7±6.5,p,<, 0.05), and up-regulated the expression of p-CREB/CREB (0.772±0.039 ,vs,. 1.014±0.093,p,<, 0.05). The results suggest that EE may alleviate irradiation-induced cognitive dysfunction and impair neurogenesis via enhancing hippocampal p-CREB expression.

关键词

丰富环境海马区环磷酸腺苷应答元件结合蛋白(CREB)认知功能障碍神经发生电离辐射

Keywords

Environmental enrichmentHippocampusCyclic adenosine monophosphate (cAMP) response element binding protein (CREB)Cognitive dysfunctionNeurogenesisIonizing radiation

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