Effect of CpG-ODN on spleen damage after carbon ion radiation

ZHANG Chao; FU Hua; YI Yonghua; CAI Jianming; GAO Fu

doi:10.11889/j.1000-3436.2022-0116

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Effect of CpG-ODN on spleen damage after carbon ion radiation

RADIOBIOLOGY AND RADIOMEDICINE | 更新时间：2023-08-29

- Effect of CpG-ODN on spleen damage after carbon ion radiation
- Journal of Radiation Research and Radiation Processing Vol. 41, Issue 4, Pages: 40301(2023)
- 作者机构：
  
  1.中国人民解放军联勤保障部队第九四〇医院放疗科兰州 730050
  2.中国人民解放军海军军医大学放射医学教研室上海 200433
- 作者简介：
  
  ZHANG Chao (male) was born in 1984, and obtained his doctoral degree from Naval Military Medical University in 2014, associate chief physician, engaged in tumor radiotherapy
  FU Hua, master degree, associate chief physician, E-mail: 47723515@qq.com
- 基金信息：
  
  Natural Science Foundation of Gansu province(22JR5RA003);National Natural Science Foundation of China(81501623)
- DOI：10.11889/j.1000-3436.2022-0116
  CLC：
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ZHANG Chao, FU Hua, YI Yonghua, et al. Effect of CpG-ODN on spleen damage after carbon ion radiation. [J]. Journal of Radiation Research and Radiation Processing 41(4):040301(2023)
DOI：

ZHANG Chao, FU Hua, YI Yonghua, et al. Effect of CpG-ODN on spleen damage after carbon ion radiation. [J]. Journal of Radiation Research and Radiation Processing 41(4):040301(2023) DOI： 10.11889/j.1000-3436.2022-0116.

摘要

本次研究主要探讨CpG-ODN对重离子辐射所致脾脏损伤的救治作用。实验中C57BL/6L小鼠受到5 Gy ,12,C,6+,离子全身照射，在照射后急性期计算小鼠脾脏指数，苏木精-伊红（Hematoxylin-Eosin staining，HE）染色观察脾脏组织结构变化。另外，免疫组化法检测γ-H,2,AX蛋白含量以判断脾脏细胞DNA发生双链断裂（（Double-strand break，DSB）情况，末端脱氧核苷酸转移酶介导的dUTP缺口末端标（Terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling，TUNEL）法检测脾脏细胞凋亡水平，荧光标记法检测外周血CD19,+,B细胞数量。结果显示，经,12,C,6+,离子照射后CpG-ODN处理，小鼠脾脏指数提高，脾脏红髓萎缩程度减轻，白髓面积增大，其内淋巴B细胞排列密度提高，发生DSB和凋亡的细胞数量减少，外周血CD19,+, B细胞数量增加。这些表明CpG-ODN能减轻,12,C,6+,离子照射后脾脏损伤，其原因可能与CpG-ODN抑制细胞发生DSB和凋亡有关。

Abstract

The purpose of this study was to explore the ameliorative effect of CpG-ODN on spleen damage after carbon ion radiation (CIR). In the experiment, C57BL/6L mice were irradiated with 5 Gy of CIR and the index of spleen was calculated. Changes in spleen tissue structure were observed by histological examination (HE). Double-strand break (DSB) of DNA and apoptosis were detected by γ-H,2,AX immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling (TUNEL) assay, respectively. The number of B cells in the peripheral blood was examined using CD19 antibody labeling. These results showed that CpG-ODN improved the index of spleen, relieved red pulp atrophy, and increased the area of white pulp, in which the number of γ-H,2,AX foci and TUNEL-positive cells were decreased after CIR. In addition, CpG-OND increased the number of CD19,+, B cells in peripheral blood after CIR. These results indicated that CpG-ODN ameliorates spleen damage induced by CIR, possibly by inhibiting the rate of DSB and apoptosis.

关键词

碳离子辐射CpG-ODN脾脏凋亡双链断裂

Keywords

Carbon ion radiation (CIR)CpG-ODNSpleenApoptosisDouble-strand break (DSB)

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