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Establishing the fingerprints of
Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components- Journal of Radiation Research and Radiation Processing Vol. 41, Issue 5, Pages: 50303(2023)
- 作者机构：
1.四川省原子能研究院 成都 610101
2.辐照保藏四川省重点实验室 成都 610101
3.成都理工大学，核技术与自动化工程学院 成都 610059
- 作者简介：
QIU Yalu (female) was born in August 1995, and obtained her master's degree from Heilongjiang University in June 2021, intern professor
GAO Peng, master's degree, associate professor, E-mail: ppenggao@163.com
- 基金信息：Sichuan Science and Technology Innovation Seedling Project(2021JDRC0121;2022JDRC0120);General Program of Sichuan Natural Science Foundation(2022NSFSC0593);Regional Innovation Cooperation in Sichuan Province(2022YFQ0044);Sichuan Science and Technology Innovation Talent Project(2021JDRC0042);Chengdu Longquanyi District Science and Technology Plan Project(2022-34-2)
DOI：10.11889/j.1000-3436.2023-0007
CLC：
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QIU Yalu, GAO Peng, HE Jiang, et al. Establishing the fingerprints of
Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components. [J]. Journal of Radiation Research and Radiation Processing 41(5):050303(2023)QIU Yalu, GAO Peng, HE Jiang, et al. Establishing the fingerprints of
Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components. [J]. Journal of Radiation Research and Radiation Processing 41(5):050303(2023) DOI： 10.11889/j.1000-3436.2023-0007.
摘要
为了建立川芎指纹图谱，考察不同剂量,60,Co-γ辐照对川芎8种有效成分的影响，以川芎为材料，采用SHIMADZU Shim-pack GIST C18色谱柱（250 mm×4.6 mm，5 μm）对8种有效成分的标准品和对照品储备液进行分析。设定不同剂量（3 kGy、6 kGy、10 kGy、15 kGy）,60,Co-γ辐照处理川芎，处理后样品通过液相色谱法得到指纹图谱。采用《中药色谱指纹图谱相似度评价系统（2004A版）》对相似度进行评价。结果表明：川芎各成分相似度均大于0.988；在低于10 kGy的射线辐照处理后，川芎的8种有效成分含量无明显差异。结果表明该方法稳定可靠。该研究为辐照技术在川芎品质保障及川芎药材辐照标准的制定提供理论依据和支撑。
Abstract
Herein, we established the fingerprint of ,Ligusticum chuanxiong, and the effects of different ,60,Co-γ irradiation doses on the eight active components of ,Ligusticum chuanxiong, were investigated. A stock solution of the eight standard reference substances in ,Ligusticum chuanxiong, were analyzed using a SHIMADZU Shim-pack GIST C18 chromatographic column (250 mm × 4.6 mm, 5 μm). Different doses (3 kGy, 6 kGy,10 kGy and 15 kGy) of ,60,Co-γ irradiation were used to treat ,Ligusticum chuanxiong,. After these treatments, the fingerprint of ,Ligusticum chuanxiong, was obtained via liquid chromatography. The similarity was evaluated using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2004 A edition), and the results indicated that the similarity of each component in ,Ligusticum chuanxiong, was ,>,0.988. After irradiation at ,<,10 kGy, the contents of the eight active components in ,Ligusticum chuanxiong, exhibited no evident differences, and the results indicated the stability and reliability of the method. Thus, this study provides a theoretical basis and support for the use of irradiation technology in the quality assurance of ,Ligusticum chuanxiong, and the formulation of irradiation standards for ,Ligusticum chuanxiong,.
关键词
川芎60Co-γ辐照有效成分指纹图谱
Keywords
Ligusticum chuanxiong60Co-γ irradiationActive ingredientsFingerprints
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