最新刊期
      期 5 ， 2007
      • LI Zhihong,WANG Junlin,ZHANG Lihua,CHEN Xinfang
        Vol. 25, Issue 5, Pages: 257-260(2007)
        摘要：By the methods of gel determination and WAXD (Wide Angle X-ray diffraction), the irradiation crosslinking rule of PA1010 and PA1010 containing multifunctional crosslinking agent BMI, the changing of G(x) and the degree of crystallisity of samples with the absorbed doses has been studied. The effect of irradiation on the destruction of crystalline is also explored. It has been shown that multifunctional crosslingking agent not only improves irradiation crosslinking structure, but also accelerates and cut down the doses for the origination destruction of crystalline. As itexists mainly in the interface region, BMI can restrain decomposing of stress bond, enhance the interface crosslinking, and result in the accumulation of defects on the surface of the crystal and the descending of Wc, which indicates that irradiation damage occurs gradually from the surface to the inside of the crystal. In the studies of Wc, Lhkl and D, it has been found that the increasing of Wc and Lhkl during early stage of irradiation has some relationship with the strain rearrangement caused by hydrogen bond and the breaking of stress bond, meanwhile changing rates of Wc and Lhkl at high irradiated dosage are correlative to amount of ratio surface of crystal, and face 010 is easier to be damaged than face 100.  
        关键词：Irradiation crosslink;Enhanced crosslink;PA1010;Destruction of crystalline   
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        发布时间：2023-03-30
      • YU Long,SUN Yang,XIE Fei,LIU Yang,AN Xiao
        Vol. 25, Issue 5, Pages: 261-265(2007)
        摘要：As a novel method of mutation breeding, the low energy ion beam implantation has been widely used. The biological effects of Bacillus coagulans implanted by Kaufman source and dual-Panning source have been compared. The results showed that with the same extraction voltage, the genetic stability of the third generation strain implanted by Kaufman source was 30% higher than that implanted by dual-Panning source, while the general mutation rate of the former was 2% lower than the latter. The appropriate ion source should be chosen to meet the requirement of mutation.  
        关键词：Kaufman sources;Dual-Panning sources;Ion implantation;Bacteria   
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        发布时间：2023-03-30
      • GUO Chaojiang,QIAO Hongqun,LI Sha,LI Hui,XU Hong
        Vol. 25, Issue 5, Pages: 266-270(2007)
        摘要：Breeding of welan gum-producing bacteria by low energy N+ ion implantation has been investigated. A high production mutant NX-3-1 for welan gum was screened under the implantation conditions of 20keV, 20×1014 /cm-2, the average production of welan gum with NX-3-1 has been raised by 27.5% compared to the original strain in shaking flask culture. NX-3-1 grew much faster than original strain companied with higher glucose consumption in 7.5L fermenter. The maximum welan gum concentration with NX-3-1 was 26.4 g • L-1.  
        关键词：Ion implantation;Welan gum;Mutation breeding;Fermentation   
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        发布时间：2023-03-30
      • WEI Shenglin,WU Lijun,YU Zengliang
        Vol. 25, Issue 5, Pages: 271-274(2007)
        摘要：In order to study the effects of N+ implantation on osmosis stress resistance of plant, the experiment was taken with liquorice as plant model and 15% PEG as the osmosis stress agent. The results showed that the stem height growth of liquorice increased by 40.2% compared with controls (p<0.01 and internal polysaccharide in leaves increased by 84.8% (p<0.01 after 20keV nitrogen ions implanted. The stem height growth showed evidently the positive correlation with the in internal polysaccharide in leaves under osmosis stress (r=0.7166, p<0.01) The selected N+ implantation parameters may be useful to increase osmosis stress resistance cultivation of liquorice and to make it mutatedwith ions beam implanation.  
        关键词：Liquorice;Osmosis stress;N+ implantation;Polysaccharide   
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        发布时间：2023-03-30
      • SUN Guisheng,ZU Jianhua,LIU Xinwen,TONG Long
        Vol. 25, Issue 5, Pages: 275-278(2007)
        摘要：The surface of nano silicon dioxide was modified by silane coupling agentto produce vinyl. Then glycidyl methacrylate (GMA) monomer was grafted to modified nano silicon dioxide by use of γ-irradiation. Effects of monomer concentration and irradiation dose on the grafting yield were studied. It was found that for simultaneous irradiation method, the reaction conditions had a complex effect on grafting yield. Fourier Transform Infrared Spectroscopy(FT-IR), Simultaneous Thermal Analysis (TGA), Transmission Electron Microscope (TEM) had been characterized the composition and structure of grafted nano silicon dioxide.  
        关键词：Nano silicon dioxide;Radiation graft;Glycidyl methacrylate   
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        发布时间：2023-03-30
      • ZHAO Yongfu,HA Yiming,LIU Ting,WANG Rongfu,WANG Changbao
        Vol. 25, Issue 5, Pages: 279-282(2007)
        摘要：Electron spin resonance (ESR) spectroscopy is one of the most effective technique for detection of irradiated food containing bone. It was found that the radiation –induced ESR signal (Spectrum, g factor and peak-to-peak line width ΔH) in bone before and after irradiation was significantly different and could be easily distinguished from the endogenous ESR signal. Sample preparation studies showed vacuumdrying and grinding at frozen temperature was an ideal method. A linear relationship was observed between ESR signal intensity and the absorbed dose (0.3-10.1kGy). It can be proposed that 0.5kGy absorbed doses can be detected by ESR for irradiated food containing bone though detecting sensitivity is very different at the same irradiated dosage with different food such as pork, beef, duck, chicken and fish .The ultimate purpose of this work is to establish a national criterion for detection of irradiated foodstuffs by use of ESR.  
        关键词：Electron spin resonance (ESR);Bone;Irradiated food;Detection;Food containing bone   
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        发布时间：2023-03-30
      • QI Bo,LIU Qingfang
        Vol. 25, Issue 5, Pages: 283-288(2007)
        摘要：Based on ISO11137 and ISO10993, bio-burden of the coronary artery stents and the sterilizing dose were determined. Results indicted that when the average bio-burden was 332.49cfu per device, verification dose was 9.5292kGy (SAL10-2) the minimum sterilizing dose was determined to be 23.1kGy (SAL10-6). The irradiated samples were inspected their biocompatibility by the cytotoxicity test, sensitization test, local reactions of muscle embedding test, chromosome aberration test of human blood cell in vitro, haemolysis test. It was showed that the coronary artery stents sterilized by irradiation have no cytoxicity, no sensitivity, no mutagencity in chromosome aberration test and no haemolysis. Furthermore, the package kept sterile and no leakage was found. The good biocompatibility of coronary artery stents sterilized by irradiation can be used for further clinic application.  
        关键词：Biocopatibility;Genotoxicity;Cytotoxicity;Ensitization;Radiation sterilization;Coronary artery stents   
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        发布时间：2023-03-30
      • SHI Zhige,BAO Shiyao,TIAN Ye
        Vol. 25, Issue 5, Pages: 289-293(2007)
        摘要：The whole brain of SD rats was irradiated by the single dose of 2, 10, or 30Gy. The enzyme-linked immunosorbent assay was used for the content measurement of myelin basic protein (MBP) in telencephalon tissue at 1 week, 1 month, 3 months and 6 months after irradiation. Both the Luxol fast blue staining with image analysis and the electron microscope were used to investigate the histomorphologic and ultrastructural characteristics of demyelination. The MBP content of telencephalon tissue in control rats were 78-82µg/mL, there were no difference in all the 2Gy irradiated, 1 week and 1 month after 10 to 30Gy irradiated groups. But at 3 and 6 months after 10Gy and 30Gy irradiated rats, there MBP content were in 50-62µg/mL level, which were a significant decrease compared with the control groups (p<0.01). Typical demyelination in corpus callosum of rats was observed in 30Gy irradiation after 6 months, but no evidence of demyelination was seen in all the other rats. The ultra-structural changes of myelin and oligodendrocytes were detected in 10Gy and 30Gy exposure after 1 to 6 months observed by electron microscope. All the demyelination changes were seen correlated with the dosage and duration after irradiation. These findings indicate that the radiation-related molecular and pathological characteristic changes of demyelination can be assessed in 3 to 6 months after single 10Gy to 30Gy whole-brain irradiation in SD rats.  
        关键词：Ionizing irradiation;Brain;Demyelination;Myelin basic protein;Rat   
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        发布时间：2023-03-30
      • NIE Jihua,DING Jiansong,CAO Yi,LI Jianxiang,ZHANG Suping,TONG Jian
        Vol. 25, Issue 5, Pages: 294-296(2007)
        摘要：The study of biological effects induced by microwave (900MHz) on SOD (Superoxide dimutase) and ODC (Ornithine decarboxylase) activity in chick embryos’ brain, and spontaneous activity of the newborn chicken has been reported in this paper. Thirty chick embryos were randomly divided into 6 groups, 3 control groups and 3 exposure groups. 3 exposure groups were irradiated with 900MHz microwave 2h per day from 5 to 20 day. The SOD and ODC activity in chick embryos’ brain was examined at 13th and 20th day of the incubation and at the second day after birth spontaneous activity was monitored. The SOD activity of the exposed groups significantly decreased at the 13th day and was recovered at the 20th day. The spontaneous activity in chicken of the exposure group increased significantly compared to the control group. Microwave would induce oxidative stress in chick embryos and enhance spontaneous activity of the chicken hatched.  
        关键词：Embryo;Microwave;Superoxide dimutase;Ornithine decarboxylase;Spontaneous activity   
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        发布时间：2023-03-30
      • ZHOU Yongjun,NIU Zhongqi,LU Zhiyuan
        Vol. 25, Issue 5, Pages: 297-301(2007)
        摘要：The interactions between the electromagnetic field and the biology system can cause the variation in conformations organizations, function and other aspects of different biological targets. When domestic sewage disinfected subsequently by constant electromagnetic wave, the microcosmic molecule with certain electric dipole-moment and biopolymer that has dielectric properties in cell may cause dielectric wastage under the outward electromagnetic field. And thermally biological effect will be produce because the energy of the microwave can be transformed as the thermal energy of movement which makes biology system temperature risen. Under the effect of the electromagnetic field in addition, the variety of the velum’s voltage not only makes the structures of cell membrane change and the gates of K+, Na+ open but also makes the gates of Ca2+ open depending on the voltage. A large amount of Ca2+ out of the velum could come into the cell at the voltage grad and induce the dead of the cells, which cause the biological non-heating effect. The larger variety the membranaceous voltage, the more cells would die. It has been shown that microwave secondary disinfection is simple method with high efficiency, lower price, easy to automatization, no additional and no secondary pollution.  
        关键词：Domestic sewage;Secondary disinfection by microwave;Mechanism;Voltage of the membrane;Biological heating effect and biological non-heating effect   
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        发布时间：2023-03-30
      • GAO Ling,LI Fengsheng,CHEN Xiaohua,DONG Bo,ZHANG Junquan,RAO Yalan,CONG Yue,MAO Bingzhi
        Vol. 25, Issue 5, Pages: 302-307(2007)
        摘要：To construct erythroblastic leukemia viral oncogene homolog 2 (ErbB2) small interference RNA ( siRNA) expression vector and to study its effect on U251 cell line proliferation and apoptosis combining with 60Co γ-iradiation. ErbB2 specific 19bp oligonucleotides were designed and synthesized. These oligonucleotides were annealed to form the double strand DNA fragments,which was cloned into pSilence2.1-U6-H1 vector. The recombinant pSilence2.1-ErbB2 expression construct was confirmed by Hind III and BamH I double digestion and sequencing. The pSilence 2.1-ErbB2 was transfected into U251 cell. Cellular proliferation activities were assayed by tetrazolium bromide (MTT) colorimetry. The apoptosis of transfected U251 cell was examined with Hoechst 33258 staining and Annexin-V kit. Psilence 2.1-ErbB2 expression vector was successfully constructed and it can effectively inhibit proliferation(p<0.05) and induced time dependent apoptosis(the percentage of apoptosis cell increase 10.52% after 24h transfection, the percentage of apoptotic cell increase 50.65% after 48h transfection) in transfected U251 cell line compared with non-transfected and pSilence2.1-GFP U251 cells, After combination with 60Co γ-irradiation, the effect of inhibiting proliferation was more significant compared with non-irradiated U251 cells(p<0.05). The pSilence 2.1-ErbB2 expression vector can effectively inhibit proliferation and induced U251 cells time dependent apoptosis; combination of 60Co γ-irradiation can enhance the inhibitory efficiency in U251 cell line.  
        关键词：Small interfering RAN;Erythroblastic leukemia viral oncogene homolog 2;U251 cell line   
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        发布时间：2023-03-30
      • XING Chungen,LI Guoqiang,ZHOU Liying,JIANG Yinfen,LIU Yulong,XUE Yongquan,LU Xueguan,WU Yafang,DAI Hong,TIAN Ye
        Vol. 25, Issue 5, Pages: 308-311(2007)
        摘要：To study the correlation between the number of radiation-induced translocations of chromosome 2 and the cell survival fraction after irradiation using fluorescence in situ hybridization , and discuss the feasibility to predict the radiosensitivity of human colorectal cancer cell using FISH. The result shows that The growth of implanted tumor had been restrained after irradiation. The sensitivity of Lovo cell was higher than SW480. The result of FISH detection to implanted tumor resected indicated that the number of chromosome 2 aberration of Lovo cell was higher than SW480 significantly (p<0.05). Fluorescence in Situ Hybridization can be used to predict the intrinsic radiosensitivity of human colorectal cancer cell conveniently and reliably. It provides important references for the selection of radiotherapy in the patients suffering colorectal cancer clinically.  
        关键词：Fluorescence in situ;Hybridization,Prediction;Colorectal cancer;Radiosensitivity   
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        发布时间：2023-03-30
      • FENG Jiangbin,LU Xue,CHEN Deqing,LIU Qingjie
        Vol. 25, Issue 5, Pages: 312-315(2007)
        摘要：This study is try to explore if the mitochondrial DNA 889bp and 3895bp deletions in human lymphoblastoid cell line could be induced by ionizing radiation. Human normal lymphoblastoid cell line samples were exposed to 10Gy 60Co γ-rays in vitro, and pure mitochondrial DNA were extracted at 24h and 48h after irradiation respectively. A nest-PCR method was used to analyze the 889bp or 3895bp deletions before and after exposure. The final PCR products were purified, sequenced and BLASTed on standard human mitochondrial genome sequence database. The results showed that the 889bp and 3895bp deletion were not detected for the cell line samples not exposed to 60Co γ-rays. The 889bp and 3895bp deletions were detected on samples exposed to 10Gy 60Co γ-rays, either at 24h or 48h after irradiation respectively. The BLAST results showed that the 889bp and 3895 deletions flanked 11688bp-12576bp, 548bp-4443bp with the piece of AAGCTTCA & CCCATACCCCGAA, respectively. These observations suggested that the 889bp and 3895bp deletion could be induced by ionizing radiation on lymphoblastoid cell lines samples.  
        关键词：Ionizing radiation;Lymphoblastoid cell line;Mitochondrial DNA deletion   
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        发布时间：2023-03-30
      • XU Wei,WANG Peng,ZHANG Xing
        Vol. 25, Issue 5, Pages: 316-320(2007)
        摘要：This paper focused on the mutagenicity of microwave irradiation with low-power on Lactobacillus casei subsp. rhamnosus 6013 cooled with circulating water of 20℃. In the parallel experiments by adjusting the power and irradiation time, a mutant strain W4-23 with L-lattic acid production of 124.15g/L higher than 80g/L of the parent strain had been obtained. And the properties of W4-23 with highly producing L-attic acid remained stable after cultured for 8 times and stored for two months at 4℃. It was concluded that the mutagencity could be stimulated under the microwave power of 400W and irradiation length of 20min.  
        关键词：Microwave irradiation;mutagenic effects;L-attic acid;Lactobacillus casei   
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        发布时间：2023-03-30
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