最新刊期
      期 2 ， 2009
      • LI Pengbo,YAN Hongxia,SONG Changwen,ZHANG Yi
        Vol. 27, Issue 2, Pages: 65-69(2009)
        摘要：Compared with traditional methods, microwave technology has advantages in promoting chemical reactions as it can improve the yield of reaction, decrease the reaction time and prevent the side reaction. In this paper, the research progresses of microwave technology applied in the synthesis, purification and modification of CNTs are discussed, in addition, the advantages of microwave technology compared with traditional methods are concerned. At last prospects for research direction are suggested.  
        关键词：Microwave technology;Carbon nanotubes;Synthesis;Purification;Modification   
        2
        |
        0
        |
        3
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833269 false
        发布时间：2023-03-30
      • WU Guozhong,TANG Zhongfeng,WANG Mouhua
        Vol. 27, Issue 2, Pages: 70-74(2009)
        摘要：Polytetrafluoroethylene (PTFE) is a high-performance engineering plastic and known as a typical material of radiation degradation. PTFE can be degraded by radiation under various conditions and PTFE micro-powder is usually fabricated by a combination of radiation and milling. PTFE can also be crosslinked by irradiation in the melt state (330~340 ℃). The materials can be applied as a special additive due to its excellent wear resistance. Crosslinked PTFE may also be applied in lithography and fuel cell membrane in the future. In this paper, history and application of PTFE degradation and crosslinking products are reviewed.  
        关键词：High temperature irradiation;Polytetrafluoroethylene;Radiation crosslinking;Radiation degradation   
        3
        |
        1
        |
        4
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833268 false
        发布时间：2023-03-30
      • PENG Chaorong,WANG Jingxia,CHEN Zhuping,WANG Xiuying,HUANG Cheng,CHEN Hao
        Vol. 27, Issue 2, Pages: 75-78(2009)
        摘要：Poly (L-lactic acid) grafting with N-vinylpyrrolidone (PLLA-g-PVP) was prepared by radiation in the presence of methanol as a solvent. The effect of absorbed dose on grafting ratio was investigated and the structures of PLLA-g-PVP were characterized by 1H-NMR, FT-IR and DSC. It shows that grafting ratio increases with increment of absorbed dose and grafting ratio reach 14.9 % at the absorbed dose of 5 kGy. The peaks of C=O stretching in chain of NVP occur in 1660~1671 cm-1, which shift to higher wave numbers increasing with absorbed dose. In 1H-NMR, the area ratio of methyl to methine is more than 3:1 which indicates the position of grafting reaction may locate in methine attaching to methyl of PLLA. Enthalpy of PLLA increases with increment of absorbed dose, and Enthalpy of PLLA-g-PVP decreases with increment of absorbed dose.  
        关键词：γ-irradiation;Poly (L-lactic acid);N-vinylpyrrolidone;Grafting   
        2
        |
        0
        |
        3
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833280 false
        发布时间：2023-03-30
      • JI Yuling,LI Linfan,YU Yang,XIE Leidong,DENG Bo,LI Jingye
        Vol. 27, Issue 2, Pages: 79-83(2009)
        摘要：Polyvinylidene fluoride PVDF powder was chosen as the base material, and grafted with styrene monomer under pre-irradiation graft polymerization method. The styrene grafted PVDF (PVDF-g-PS) powder was characterized by FTIR spectroscopy, DSC, TGA and element analysis. The FTIR spectra demonstrate that the polystyrene is successfully grafted into PVDF powder. The crystallinity of grafted PVDF powder decreases with the increasing in the degree of grafting according to the DSC curves. The TGA curves of PVDF-g-PS change remarkably with respect to the original PVDF. It further proves the polystyrene is successfully grafted into PVDF powder.  
        关键词：PVDF powder;Styrene;Radiation grafting   
        3
        |
        1
        |
        6
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833281 false
        发布时间：2023-03-30
      • SU Xiuxia,ZHU Xiaofeng,LI Zhongjin,WANG Lei,DUN Xueming,LU Kuan
        Vol. 27, Issue 2, Pages: 84-88(2009)
        摘要：Xanthan gum-graft-Acrylamide (XG-g-AM) was synthesized by 60Co γ-rays radiation. The effects of the different reaction conditions on the grafting degree and the grafting efficiency were explored. The structures of the grafted copolymers were characterized by means of Element Analysis (EA), Fourier-Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Thermal Gravimetric Analysis (TGA) respectively. The experiment results show that the optimized grafting reaction parameters, XG concentration of 10 g/L, AM and XG Mass ratio of 3:1, the total absorption dose of 6.0 kGy can make the grafting degree up to 128.5 % and the grafting efficiency to 80.4 %. And the flocculation performance of XG-g-AM has been also confirmed.  
        关键词：Xanthan gum;Acrylamide;60Co γ-rays radiation grafting;Flocculation performance   
        2
        |
        0
        |
        6
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833279 false
        发布时间：2023-03-30
      • BAI Yanhong,LI Quanshun,MAO Duobin,ZHAO Dianbo,ZHANG Xiaoyan,YANG Gongming
        Vol. 27, Issue 2, Pages: 89-94(2009)
        摘要：The effects of electron beam irradiation on the sterilization and preservation of chilled pork were studied. The aim of this investigation was to provide academic and technical basis for application of electron beam irradiation on meat industry. The response surface analysis was used with electron beam energy(X1) and dose(X2) as factors and colony form unit(Y) as responses. The results have been shown that the model of sterilization of chilled pork by electron beam irradiation can be expressed Y=3.78-0.24X1-0.13X2-0.16X1X2-0.18X12+0.15X12(R2=0.9755). It has been found there is a interaction between electron beam energy and absorbed doses, and the significance sequence of factors is absorbed dose>interaction> electron beam energy. When absorbed doses are in range from 3.23 kGy to 4.0 kGy and electron beam energy is in range from 2.3 MeV to 3.8 MeV, the colony form unit would drop 2 logarithm units. The shelf life of samples treated with electron beam irradiation is longer by about 12 d than that of control samples when the samples are stored at 4 ℃. When the samples are stored at 7~10 ℃, shelf life of samples treated with electron beam irradiation is longer by about 9 d than that of control samples. The results showed that electron beam irradiation has the effects of sterilization and preservation on chilled pork. This study has been confirmed that the application of electron beam irradiation is very useful for meat industry.  
        关键词：Electron beam radiation;Chilled pork;Sterilization;Shelf life   
        2
        |
        0
        |
        3
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833372 false
        发布时间：2023-03-30
      • TANG Jinglong,LIU Guoquan,XIONG Ling,WANG Shuo,WANG Jianyu,LIU Li,LI Jiage,YUAN Fuqiang,XI Tingfei
        Vol. 27, Issue 2, Pages: 95-100(2009)
        摘要：The present study was designed to investigate the distribution, accumulation and excretion of hydroxylapatite nanoparticles (nano-HAP) in mice with intravenous injection. The nano-HAP suspension was neutron activated and then stored 151d after neutron activation for experiment. The mice injected with radioactive nano-HAP heve sacrificed at 15min、1h、2h、4h、8h、24h、72h and the main organs were harvested for further measuring the counts of 45Ca in every sample by use of a liquid scintillation counter. Results indicate that 45Ca could be detected in all main organs (0.5~250Counts/min•mg), and there are more 45Ca exist in lung, liver and spleen. The counts of 45Ca in bone almost keep the same during the whole experiment period while counts of 45Ca of all other tissues decrease with time. And the counts of 45Ca exerted in faeces are higher than that in urine. So we can conclude that nano-HAP distribute in all organs of mice especially in lung, liver and spleen. But bone is the only tissue in which nano-HAP accumulated. Nano-HAP was excreted mostly through faeces.  
        关键词：Hydroxylapatite nanoparticle;Distribution;Neutron activation;Mice   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833376 false
        发布时间：2023-03-30
      • LI Yanbo,GUO Caixia,WANG Zhicheng,LI Pengwu,GUO Wei,ZHAO Hongguang,LIU Yang,GONG Shouliang
        Vol. 27, Issue 2, Pages: 101-105(2009)
        摘要：In order to explore the effect of multiple low dose irradiation (LDR) on the apoptosis of splenocytes, immune factors and lymphocyte subgroups in twelve-week diabetes mellitus (DM), the experiment was carried out with control, DM and DM+LDR groups. The irradiation dose every time was 25, 50 and 75 mGy respectively, and the irradiated times were 15. At the eighth weekend after the DM rats were irradiated, the percentages of CD4+, CD8+ T lymphocytes and TCR α β were detected by flowcytometry (FCM). The content of IL-2 in both serum and supernatant of cultured splenocytes were detected by ELISA. And the apoptotic rate of splenocytes was detected by FCM and TUNEL respectively. The result shows that as compared with that in the control, the body weight (BW) decreases both in the DM and the DM + LDR groups, particularly in the DM group. The blood glucose (BG) level in the DM+LDR groups is higher than that in the control, but lower than that in the DM group. As compared with those in the control, the percentages of TCR α β and CD4+ T cells, the content of IL-2 in serum and supernatant of cultured splenocytes, and the apoptotic rate of splenocytes in DM + LDR groups increase significantly. However, as compared with those in the DM group, the percentages of TCR α β, CD4+ and CD8+ T cells and the splenocyte apoptotic rates in the DM+LDR groups decrease while the content of IL-2 and the ratio of CD4+/CD8+ T cells increase. It is obvious that the multiple LDR could regulate and weaken the loss of BW and increase of BG caused by DM, correct the imbalance of lymphocyte subgroups and immune factors, decrease the increment of splenocyte apoptosis resulted from DM. Multiple LDR could result in body protection.  
        关键词：Low dose radiation;Diabetes mellitus;Lymphocyte;Spleen;Apoptosis;IL-2   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833386 false
        发布时间：2023-03-30
      • ZHOU Liwei,CHEN Xiaoming,ZHANG Jianguo,LIU Fang,ZHANG Genfa
        Vol. 27, Issue 2, Pages: 106-110(2009)
        摘要：In order to investigate the DNA damage exposed to ultraviolet-C(UVC) and the effect both of detecting method and sample types on the result, the Bacillus subtilis and general DNA were used as the model samples irradiated with different doses of UVC respectively. Subsequently, DNA fragments were analyzed by 8 h, 16 h, and 24h pulsed-field gel electrophoresis (PFGE). The result has been revealed that the 16 h PFGE could reflect the DNA double strand break (DSB) more exactly. With the analysis of 16 h PFGE map it has been found that the percent of DNA release increases gradually with the increment of radiation doses. What's more, the most DSB appear at the dose of 17.8 J/cm2 in the thallic samples and at the dose of 72.7 J/cm2 in the DNA samples. The DSB is more significant in the thalli than in general DNA. The results indicate that the DSB level induced by UVC is related with irradiation doses as well as sample types.  
        关键词：UVC radiation;Bacillus subtilis;DNA double-strand breaks;Pulsed-field gel electrophoresis (PFGE)   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833399 false
        发布时间：2023-03-30
      • XU Xiaoya,JIN Weifang,GAO Jianjun,ZHOU Tie,SHENG Hui
        Vol. 27, Issue 2, Pages: 111-114(2009)
        摘要：In order to investigate focal flushing dose radiation effect on the generation, differentiation and gene expression of bone marrow stromal cells the femoral head of rats was irradiated at 30 Gy by 137Cs γ-rays (dose rate: 0.83 Gy/min). Then the bone marrow stromal cells (BMSCs) was cultured. The ability of proliferation and colony formation was observed and the expression level of Cbf-α1, PPAR-γ, VEGF-a and KDR was detected by RT-PCR technology in two weeks later. It has been found that after partly irradiated by 137Cs γ-rays the proliferation and the number of colony of BMSCs in irradiated group decreases obviously meanwhile the expression level of Cbf-α1, PPAR-γ and VEGF-a in irradiated BMSCs obviously decreases by 18.98 %, 9.46 %, 57.34 % and 5.56 % respectively compared to the normal BMSCs (p<0.05). It shows that the focal great radiation could damage the BMSCs and depress the generation, differentiation and the expression of the related genes obviously.  
        关键词：Radiation;Bone marrow stromal cells (BMSCs);Core binding factor alpha 1(Cbfα1);Peroxisome proliferator-activated receptor-γ(PPAR-γ);Vascular endothelia growth factor (VEGF)   
        2
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833384 false
        发布时间：2023-03-30
      • HUANG Yuecheng,SHAN Husheng,LIU Hanchen,CAI Jianming,CUI Jianguo,SUN Ding,LI Bailong,ZHAO Fang,DONG Junrui,GAO Fu
        Vol. 27, Issue 2, Pages: 115-119(2009)
        摘要：In this paper the carboxyl-fullerene C3 was synthesized using Bingle’s cycloaddition reaction and its molecular structure was characterized by 1H-NMR, 13C-NMR and MS-ESI. The free radical scavenging ability of C3 was confirmed by ESR in Fenton system. CCK-8 assay, AnnexinV/PI staining and flow cytometry assay were performed to evaluate the cell survival rate, apoptosis and cell cycle alteration after γ-ray irradiation respectively. The results showed that C3 has excellent free radical scavenging ability in Fenton system with a scavenging rate 93 % at the concentration of 1000 mg/L. It has been revealed that C3 has good radioprotection effects to AHH-1 and HIEC cells from radiation damage induced by 1~12 Gy γ-rays in evidence of higher survival and lower apoptosis rate of the cell. The mechanism may be related to its ability of scavenging free radicals.  
        关键词：Carboxyl-fullerene C3;γ-ray;Free radical;AHH-1 cell   
        2
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833447 false
        发布时间：2023-03-30
      • YANG Wei,LI Yanbo,ZHAO Jingguo,GONG Shouliang,CAO Jianping,LIU Fenju
        Vol. 27, Issue 2, Pages: 120-124(2009)
        摘要：In order to investigate the anti-tumor effect of RNA interference silencing HIF-1α and Survivin genes combined with X-ray irradiation on hypoxic SMMC-7721 cells in vitro, we detected mRNA and protein expression of HIF-1α and Survivin genes in hypoxic SMMC-7721 cells after transfection with pGenesil-Survivin-HIF carried by liposome by RT-PCR and Western blot respectively. Survival fraction and apoptosis of hypoxic SMMC-7721 cells in vitro were detected by MTT and FCM assay respectively. The results show that mRNA expression of HIF-1α and Survivin genes in hypoxic SMMC-7721 cells transfected with pGenesil-Survivin-HIF is significantly lower than that of control and negative interference group (p<0.05). Protein expression of HIF-1α and Survivin genes in hypoxic SMMC-7721 cells after transfection with pGenesil-Survivin-HIF can not be detected. Survival fraction of pGenesil-Survivin+5 Gy group, pGenesil-HIF+5 Gy group and pGenesil-Survivin-HIF+5 Gy group is significantly lower than that of 5 Gy group (p<0.01). Survival fraction of pGenesil-Survivin-HIF+5 Gy group is also significantly lower than that of other groups (p<0.01). Percentage of apoptosis of pGenesil-Survivin+5 Gy group, pGenesil-HIF+5 Gy group and pGenesil-Survivin-HIF+5 Gy group is significantly higher than that of control group (p<0.01). Percentage of apoptosis fraction of pGenesil-Survivin-HIF+5 Gy group is significantly higher than that of other groups (p<0.01). These results can be suggested that the pGenesil-Survivin-HIF could silence mRNA and protein expression of HIF-1α and Survivin genes in hypoxic SMMC-7721 cells. Anti-tumor effect of pGenesil-Survivin-HIF+5 Gy group is more powerful than that of pGenesil-Survivin+5 Gy group or pGenesil-HIF+5 Gy group with hypoxic SMMC-7721 cells in vitro.  
        关键词：RNA interference;X-ray;HIF-1α;Survivin   
        2
        |
        0
        |
        1
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833451 false
        发布时间：2023-03-30
      • MA Shuang,LI Wenjian,WANG Jufang,LU Dong,LIU Jing
        Vol. 27, Issue 2, Pages: 125-128(2009)
        摘要：Biocontrol bacterium BJ1 was irradiated with different doses of 12C6+ ion beam in order to improve the antagonistic ability, to optimize the parameters of ion beam irradiation and to obtain high effect of biocontrol strains. The results show that the survival rate of BJ1 irradiated with 100 Gy reaches the highest and the survival curve of BJ1 presents a tendency of going down first and then increasing, which takes a shape of "saddle" during 0 to100 Gy. In view of the effect of mutation induction, BJ1 irradiated with 400 Gy enhances its effect of antagonistic ability obviously. Based on the results of survival rate and the effect of antagonistic ability, an optimal treatment dose of 12C6+ irradiation is 200 to 400 Gy for BJ1. And BJ1 has been identified to be Bacillus subtilin by 16S rDNA sequence cluster analysis.  
        关键词：Ion beam irradiation;Biocontrol bacterium-BJ1;Mutation;Identification   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833450 false
        发布时间：2023-03-30
      0