最新刊期
      期 3 ， 2009
      • PAN Xiaosong,SHI Yujia,YAO Yimin,XU Hong,LIU Fenju
        Vol. 27, Issue 3, Pages: 129-132(2009)
        摘要：Natural killer cells is an important immunological factor in killing malignant cells. Low dose radiation can enhance proliferation and biological activity of NK cell. The involvement of P38MAPK signal pathway and endogenous glutathione induced by LDR may be the probable mechanism. Natural killer cell, especially adherent natural killer cell, is the preferential choice for adoptive cellular immunotherapy, which has a remarkable foreground in malignancy therapy.  
        关键词：Natural killer cell;Low dose radiation (LDR);Adoptive cellular immunotherapy (ACI)   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833539 false
        发布时间：2023-03-30
      • SUN Fang,LI Minglei,XIONG Jun,JIANG Shengling,DU Hongguang
        Vol. 27, Issue 3, Pages: 133-138(2009)
        摘要：The influence of photoinitiators and monomers on photopolymerization property of photosensitive polysiloxane urethane acrylate oligomer (PSUA) was investigated by real time Infrared spectroscopy (RTIR). The results show that initiating efficiency of cleavage photoinitiators is higher than that of abstraction-hydrogen photoini-tiators and polymerization rate of the PSUA system using Irgacure 1700 as photoinitiator is the highest. The optimum concentration of photoinitiator 1173 has been determined to be 0.5 %~1 %. Double bond conversion of PSUA system decreases with increment of functionality of added monomer. Double bond conversion of PSUA system containing HEA or IBOA is higher than that containing TPGDA or HDDA (90 %~95 %) and former are polymerized almost completely meanwhile the one containing TMPTA only reaches nearly 80 %. Polymerization rate of the PSUA systems containing different monomers increases in the following order: TPGDA≈HDDA>HEA>TMPTA>IBOA. Both of double bond conversion and polymerization rate of PSUA system decrease with increment of monomer concentration.  
        关键词：Photopolymerization;Silicone;Real time infrared spectrum (RTIR)   
        2
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833543 false
        发布时间：2023-03-30
      • ZHANG Hongru,LIU Wentao,HE Suqin,LI Zhihui,YANG Mingcheng,LI Zhongyuan,ZHU Chengshen
        Vol. 27, Issue 3, Pages: 139-144(2009)
        摘要：Nano-magnetic particles (Fe3O4) prepared by chemical-co-deposition method were modified by radiation-induced polymerization of N-isopropylacrylamide (NIPA), MBA and PEG. It has been found that the distribution of the porous magnetic particles was uniform and the equilibrium swelling ratio of porous magnetic hydrogels is larger than that of nonporous magnetic hydrogels. The losing water ratio of the porous magnetic hydrogels is 96 % which is enhanced about 76 % compared with ordinary magnetic hydrogels. And with the addition of PEG, porous magnetic hydrogels shows a LCST (low critical solution temperature) around 37 ℃ and has rapid responsibility.  
        关键词：Radiation;Magnetic hydrogels;N-isopropylacrylamide (PNIPA);Fe3O4;Rapid response   
        2
        |
        0
        |
        1
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833544 false
        发布时间：2023-03-30
      • SONG Xiyu,ZHANG Peng,LI Haixia,WANG Wenfeng
        Vol. 27, Issue 3, Pages: 145-150(2009)
        摘要：In this work the property of α-lipoic acid (LA) was studied by using pulse radiolysis and laser flash photolysis technology. The characteristics and mechanism of the reactions of LA with ROS (reactive oxygen species) were studied. Transient spectra of LA radicals were observed and the transient species with characteristic absorption spectra were identified. It has been found that LA can effectively quench active oxygen radicals such as .OH, CO3.-, SO4.-, eaq-, Br2.- at the rates of 7.4×109, 9.8×108, 2.3×109, 1.3×1010 and 2.0×109 mol-1·s-1, respectively. The relevant mechanism of the reactions has been discussed. The studies indicate that LA can quench free radicals existing in biological system effectively as a powerful antioxidant.  
        关键词：Pulse radiolysis;Lipoic acid;Antioxidant;Reactive oxygen species   
        2
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833537 false
        发布时间：2023-03-30
      • ZENG Fansong,YU Xiangzhong,LI Xiaoyan,WU Xiaoli,ZHANG Jianhua,LI Rurong
        Vol. 27, Issue 3, Pages: 151-155(2009)
        摘要：The paper introduces a method to build up a collimated multi-energy point γ-ray source for experiment with an industrial 60Co source. In details the theoretical design, measurement method of reducing scattered background greatly and measurement of flux, theoretical computation method, solution for radioprotection and weight bearing problem, and so on have been described and discussed. The experimental results show that the building of new method has better application.  
        关键词：Industrial 60Co line source;Multi-energy point;γ-ray collimated source;Scattered background;Flux   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833538 false
        发布时间：2023-03-30
      • LI Yijie,HONG Chengjiao,ZHANG Baoguo
        Vol. 27, Issue 3, Pages: 156-160(2009)
        摘要：In this paper the Ficoll method was used to isolate peripheral blood lymphocyte of rats, and then the isolated lymphocytes were treated by heat shock for 90 min under 42 ℃ and 5 % CO2. Flow cytometer was applied to detect the expression of HSP70 after heat shock treatment at different revival time. Flow cytometry Annexin V/PI was utilized to measure the apoptosis rate of lymphocytes which were already pretreated by heat shock and irradiated under 0.5 Gy, 1.0 Gy, 1.5 Gy, 2.0 Gy ,2.5 Gy, 3.0 Gy X-ray. The expression of HSP70 after heat shock treatment and the influence of HSP70 to the apoptosis rate of lymphocytes irradiated under different doses has been investigated. The results show that the expression of HSP70 in lymphocytes increases obviously at 1h and reaches the maximum at 4 h after heat shock treatment, and can maintains in 24 h. The expression of HSP70 induced by heat shock pretreatment can counteract lymphocyte apoptosis produced in irradiation by X-ray (1.0~3.0 Gy). So the irradiation by X-ray is a useful method to confirm counteraction of lymphocyte apoptosis by HSP70.  
        关键词：Heat shock protein 70;Irradiation;Flow cytometer;Apoptosis   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833625 false
        发布时间：2023-03-30
      • CHEN Chunhai,QU Zhandong,YANG Xuesen,ZHANG Guangbin,ZHONG Min,YU Zhengping
        Vol. 27, Issue 3, Pages: 161-166(2009)
        摘要：It was investigated that the influence of continuous microwave (MW) irradiation during lactation and gestation on the function of learning and memory as well as the role of FT3 and FT4 in this process. 48 SPF KM mice were divided into lactation group, gestation group and control group. All mice were irradiated by MW of 30 mW/cm2 for 20 min each day except control groups. The gestation group was continuous irradiated until childbirth, about 21days in total. The lactation group was continuous irradiated for 21 days. The blood serum was obtained on the 15 th day of irradiation and then serum FT3 and FT4 were determined by radioimmunoassay. In the gestation irradiation group, FT3 was not significantly changed while FT4 decreased compare to control group. In Place Navigation Test, the escape latency was prolonged in the gestation irradiation group, while there was not significantly changed in the lactation irradiation group. In Spatial Probe Test, the time spent in target quadrants was much longer and the frequency passing through the platform was much more compare to control group. In lactation group, no such change was observed in Morris water maze. The serum FT3 in the lactation irradiation group was decreased while FT4 did not change significantly. The results suggest that continuous MW irradiation during lactation could down regulate serum FT3 level, but could not induce learning and memory function injured. Continuous MW irradiation during gestation could down regulate serum FT4 level and induce learning and memory function injured. The serum TH decrement may be one of the main reasons for learning and memory dysfunction induced by MW.  
        关键词：Microwave;Lactation;Gestation;Morris water maze;Learning and memory function   
        2
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833628 false
        发布时间：2023-03-30
      • PANG Min,YAO Jianming
        Vol. 27, Issue 3, Pages: 167-172(2009)
        摘要：In this paper, the mutation breeding on Escherichia coli producing tryptophanase was studied after low energy N+ ion beam implantation. Parameters in the N+ ion beam implantation were firstly determined. It has been indicated that a high mutation rate of E.coli could be obtained by N+ implantation with 10 keV and 13×1014 N+/cm2 when glycerin at 15 % concentration used as protector. After continuous mutagenicity a high-yield tryptophanase-producing strain has been screened out and both of its biomass and enzymatic activity are higher than the previous levels respectively. The results of scale-up production show that the biomass could be reach 8.2 gww ·L-1 and 110 g L-tryptophan could be formed in the volume of 1L enzymatic reaction system. In addition, the characteristics of its stable descend ability and easy operation make it a promising strain for industrialization.  
        关键词：Ion implantation;L-tryptophan;Escherichia coli;Breeding   
        2
        |
        0
        |
        6
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833626 false
        发布时间：2023-03-30
      • XU Shangcheng,YU Zhengping,WU Juan,PEI Liping,LUO Xue,CHEN Chunhai,HE Mindi,WANG Yuan,YAO Quan,ZHONG Min
        Vol. 27, Issue 3, Pages: 173-176(2009)
        摘要：In order to investigate the inhibitory effect of microwave exposure on the import of mitochondrial transcription factor A (Tfam), we successfully created a recombined expression vector Tfam-pTNT, which was then placed into rabbit coupled reticulocyte lysate system to be translated into the precusor of Tfam (pre-Tfam) labeled with S35 methionine. After exposing the primary cultured cortex neurons to microwave for 15min, we isolated the mitochondria from these treated neurons at 0 h, 6 h, 12 h, 24 h and 48 h after microwave irradiation. Through import assay, it was found that microwave exposure obviously decreased the import of the pre-Tfam from cytoplasm into mitochondria in a time-depend manner. The results indicate that microwave exposure can inhibit the import of Tfam, which reveals a novel mechanism that microwave irradiation disturbs the energy metabolisms in neurons.  
        关键词：Microwave;Neurons;import assay;Tfam import   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833632 false
        发布时间：2023-03-30
      • ZHANG Liuzhen,XU Yujie,LIU Min,WENG Wanwen,DING Wenxiu,YANG Zhanshan
        Vol. 27, Issue 3, Pages: 177-181(2009)
        摘要：The elution with sodium hydroxide was used to increase deacetylation (DD) degree of chitosan and 60Co γ-irradiation was applied to decrease its molecular weight. The change of chitosan’s degree of deacetylation (DD) was determined by linear potentiometric titration, and the change of molecular weight was measured by Ubbelohde Viscometer. Fourier transform infrared spectrometer was utilized to characterize and analyze the products. The result indicates that sodium hydroxide elution could increase deacetylation (DD) degree of chitosan efficiently and 60Co γ-rays irradiation could decrease its molecular weight remarkably. The products may be satisfied with the preparation of new nano-chelating agents.  
        关键词：Chitosan;Degree of deacetylation (DD);Molecular weight;IR   
        2
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833630 false
        发布时间：2023-03-30
      • ZHOU Liwei,ZHANG Genfa,CHEN Xiaoming,ZHANG Jianguo,LIU Fang
        Vol. 27, Issue 3, Pages: 182-186(2009)
        摘要：In order to study the ultraviolet tolerance of a Bacillus subtilis radiation-resistant strain, the Bacillus subtilis radiation-resistant strain and its original Bacillus subtilis var. niger were used as the samples, and exposed to ultraviolet with different doses. The survival rate of both strains was investigated by plate counting meanwhile the DNA double strand breaks (DSBs) were analyzed by a pulsed-field gel electrophoresis (PFGE). It has been found that the ultraviolet tolerance is greater in radiation-resistant strain than that in the original strain in log phase. And the DSBs level in radiation-resistant strain is lower than that in the corresponding samples of original strain. However, the DSBs are more intense in thalline samples than that in DNA samples. It has been also indicated that the DSBs level is related closely with the absorbed dose as well as the sample types.  
        关键词：Ultraviolet irradiation;Bacillus subtilis;DNA double-strand breaks;Strain surviva rate   
        2
        |
        0
        |
        5
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833681 false
        发布时间：2023-03-30
      • LI Yu,ZHAO Fang,ZHENG Xiulong
        Vol. 27, Issue 3, Pages: 187-189(2009)
        摘要：In order to investigate radiosensitized effect induced by WORTMANNIN(WT) the nuclear factor kB (NF-kB) was studied by Electrophoretic  Mobility Shift Assay (EMSA). Double strand nucleotide sequence which contains kB genetic locus was composed, diluted and annealed in pure water, and then labeled as radioactive nucleotide probe. LP3 cell was cultivated in conventional method and receive 5 Gy of γ-irradiation after nurturing with WT. The nucleoprotein picked up from the cell was combined with the radioactive labeled probe and then was carried out with electrophoresis by SDS-PAGE, which was taken photographs using autoradiograph in low temperature after vacuum drying. It has been found that the NF-kB activity of LP3 cell arises from about 8 % to 50 % and then becomes down to 12 % in 6 h after irradiation. WT at 50 µmol/L can inhibit the NF-kB activity evidently. Radiation can activate NF-kB through message pathway and promote the gene express, which is related to repair and other defense mechanism of damaged DNA in cells. WT may block the massage pathway at early stage and induce radiation sensitized effect.  
        关键词：Wortmannin;Radiation damage;NF-kB;Electrophoretic mobility shift assay (EMSA)   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833674 false
        发布时间：2023-03-30
      • LE Chen,LI Haijun,CHENG Ying,MIN Rui
        Vol. 27, Issue 3, Pages: 190-192(2009)
        摘要：Rats were whole body irradiated by γ-rays with different doses. A commercial ELISA kit was used to analyze the concentration of MMP-2 and MMP-9 in rat’s serum. And Gelatin zymography electrophoresis was used to test the activity of serum MMPs at 24 h after irradiation. The results show that the amount and the activity of MMP-2 in rat’s serum increase with increment of irradiation doses. Compared with 1~4Gy exposed groups a significant rising of MMP-2 has been found in 5 Gy and 6 Gy exposed groups (p<0.01). On the contrast, the amount and activity of MMP-9 in rat’s serum have a little change at 24 hours after irradiation in all of exposed groups. It can be deduced that the changes with amount and activity of MMP-2 may be used as a potential indicator of exposed dose in organisms.  
        关键词：γ-rays irradiation;Matrix metalloproteinase;Bio-dose   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35833677 false
        发布时间：2023-03-30
      0