最新刊期
      期 4 ， 2009
      • WANG Zhuanzi,LI Wenjian,WEI Wei,JING Xigang,QU Ying
        Vol. 27, Issue 4, Pages: 193-200(2009)
        摘要：Either internal radiation using radionuclide or external radiation using different rays induced bystander effects have been confirmed certainly by use of the cell cultured in vitro or in vivo exposed to radiation. There are many methods to study the bystander effects, such as conventional low-dose radiation, un-irradiated cells co-cultured with irradiated cells, treatment with irradiated cells conditioned media, single-cell micro-beam irradiation, and so on. Whichever method is used depends on the laboratory conditions and research purposes. A series of summary has been made in this paper.  
        关键词：Intracellular irradiation;Conventional radiation;Microbeam radiation;Research methods   
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        发布时间：2023-03-30
      • ZHAO Weiliang,WANG Mozhen,TANG Gang,FANG Yuee
        Vol. 27, Issue 4, Pages: 201-206(2009)
        摘要：In this paper, a high-oil- absorption resin was prepared through the emulsion polymerization of lauryl methacrylate with additive of a small amount of N-hydroxymethyl acrylamide and acrylic acid, OP-10 and sodium lauryl benzenesulfate as the emulsifier, and initiated by γ-rays. Characterization analysis of the sample was carried out by FTIR, SEM and TG. SEM image shows that the morphology of the micelles from the polymer emulsion is regular, and the average particle size is about 170 nm. Polymer remains queit stable below 300oC. The influence of reaction conditions such as total absorbed dose, dose rate, solids content, N-hydroxymethyl acrylamide and acrylic acid content on kerosene absorption performances of the polymer has been studied. The maximal saturated oil absorbed ratio (g/g) of kerosene, chloroform and dimethyl benzene is 18.1, 19.5, and 15.3, respectively.  
        关键词：High-oil-absorption resin;Emulsion polymerization;γ-ray irradiation initiate   
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        发布时间：2023-03-30
      • PU Changyong,CAO Changqing,ZHANG Jing,MA Hongjuan,WANG Min
        Vol. 27, Issue 4, Pages: 207-211(2009)
        摘要：Photo-degradation of 4-chloroaniline (4-CA) in aqueous solutions was studied by UV irradiation. The degradation efficiency of 4-CA and total organic carbon (TOC) removal were investigated by changing the irradiation UV wavelength, initial concentration of 4-CA, pH value, adding H2O2 and saturated with different gases in the photo-degradation experimental system. The results show that the photo-degradation of 4-CA is very effective, with removal rates at about 99% and 50% of 4-CA and TOC, respectively, after 1 hour irradiation time when 10mmol/L H2O2 was added to 1mmol/L 4-CA aqueous solution and saturated with air, which implies that the UV irradiation technique might be a promising process for the treatment of 4-CA waste effluents.  
        关键词：UV light;4-CA;Degradation efficiency;Total organic carbon (TOC)   
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        发布时间：2023-03-30
      • WEN Fangping,WANG Dan,XU Changhe,XU Fengting,ZHANG Zhiwei,ZHANG Xiaoxue
        Vol. 27, Issue 4, Pages: 212-217(2009)
        摘要：The physiological responses to 133Cs and 88Sr stress and the characteristics of uptake and accumulation of 133Cs and 88Sr by Sorghum sudanense were studied by pot experiments. The results show that under the low concentration of 133Cs or 88Sr stress, the stress-resistance of Sorghum sudanense increases, the content of MDA decreases and the activities of POD and CAT increase. Under the high concentration of 133Cs or 88Sr stress, the lipid peroxidation are strengthened, the content of MDA increases and the activities of POD and CAT decrease. Along with the increment of concentration, the contents of chlorophyll decrease meanwhile the synthesis of chlorophyll in Sorghum sudanense is inhibited under the stress. High concentration of 133Cs and 88Sr has toxicity to Sorghum sudanense and has done the damage to the plant. However, there are no significant effects on the biomass of Sorghum sudanense. Along with the increment of concentration, the contents of 133Cs or 88Sr increase in Sorghum sudanense, and the relationship of the contents: roots> the part above-ground, 88Sr>133Cs.  
        关键词：133Cs;88Sr;Sorghum sudanense;Biochemical reactions;Accumulation   
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        发布时间：2023-03-30
      • LIN Hui,XU Yuanying,WU Dongsheng,JING Jia,XU Liangfeng
        Vol. 27, Issue 4, Pages: 218-223(2009)
        摘要：BCRT (Biologically Conformal Radiotherapy) is a hotspot in modern radiotherapy research. The implementation of BCRT concept is based on the rich knowledge on the radio-sensitivity of the cells in target. Monte Carlo method can simulate the radiation damage process economically, and output the rigorous, numeric and microcosmic damage yields. The work calculated the variation of DNA damage yields with energy of irradiation electrons using Monte Carlo codes, MCDS/MCER/VC. The results show that it is easier for the low energy electrons to damage DNA by DSB (double-strand break). The enzymatic DSB has been calculated in the base of the combination of MCDS and MCER, which show that the influence of incident electron energy variation on enzymatic DSB yield is very little, but that of different base excision repair mechanism is notable. The damage yields of 5 β-emitter radionuclides (Lu-177, Cu-67, Re-186, Re-188, and Y-90) have been deduced by weighted energy spectra, which show the Lu-177, Cu-67 with a bit lower energy than the others have a little higher DSB damage yield and lower SSB, OTHER and ALL yields. It also shows that the influence of these 5 radionuclides on DSB yield is relatively the more, but on SSB yield is relatively the less. The cell surviving fraction and the key parameters for two models have been obtained by VC code based on measured data, on which the influence of the repairing time on surviving fraction has been studied.  
        关键词：Biologically conformal radiotherapy;Monte Carlo;Damage yield;Surviving fraction;Repair time   
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        发布时间：2023-03-30
      • LIU Guosheng,HOU Ying,WU Feiyan,ZHAO Ting,WANG Xiuqiang,LI Zongyi
        Vol. 27, Issue 4, Pages: 224-228(2009)
        摘要：N+ ion implantation with different doses was used to screen sulfaguanidine resistance enhanced strains using Bacillus subtilis HSD06 as an initial strain for mutation. It has been confirmed that the best implantion dose is 3×1015 cm-2 for resistance enhanced mutants. Two strains B11 and B13 with higher yield of inosine have been obtained from 528 mutants. Shake flask test shows that the yield of inosine reaches 14.83 g/L and 14.38 g/L with B11 and B13 respectively, which has increment by 16.3% and 12.8% respectively compared with that of initial strain.  
        关键词：Inosine;Ion beam implantation;Bacillus subtilis;Sulfaguanidine resistance;Mutation   
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        发布时间：2023-03-30
      • LIANG Xiaofang,HONG Chengjiao,ZHANG Baoguo
        Vol. 27, Issue 4, Pages: 229-233(2009)
        摘要：In order to investigate the effects of Quercetin on radiosensitivity of human Uterine Cervix Cancer HeLa cells, MTT assay and clonogenic assay were performed to evaluate the cytotoxicity of Quercetin on the cells. Clonogenic assay was used to observe its effects on the radiosensitivity of the cells. MTT result shows that the inhibition of Quercetin on the cells is in the dose-dependent and time-dependent. And the clonogenic assay result shows that the effect of Quercetin on HeLa cells can be divided into two parts, one for the inhibition of HeLa cells and another for the induction of HeLa cell death. The other clonogenic assay result also shows Quercetin can decrease clonogenic survival rate of HeLa cells exposed to X rays. The study shows Quercetin might enhance the radiosensitivity of the HeLa cell line. And it may provide a useful evaluation to combination of ionizing radiation and Quercetin for cancer patients.  
        关键词：Quercetin;Cell proliferation;Radiosensitivity;HeLa cell   
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        发布时间：2023-03-30
      • ZHANG Wei,TU Yu,WANG Lili,ZHOU Juying
        Vol. 27, Issue 4, Pages: 234-238(2009)
        摘要：In order to ivestigate the effect of magnesium sulfate (MgSO4) on the inflammatory reaction, the whole brain of SD rats were irradiated with a dose of 20 Gy so as to establish radiation-induced brain injury model. The expression of intercellular surface adhesion molecule-1(ICAM-1) was measured serially with enzyme linked immunosorbnent assay (ELISA) and the activity of myeloperoxidase(MPO) in brain tissue was measured by the absorption spectrometry at 1, 7, 14 days after irradiation. The expression of ICAM-1 and the activity of MPO in brain of rat irradiated with 20Gy increase markedly. MgSO4 can singificantly down-regulation the expression of ICAM-1 and the activity of MPO of the irradiated rats, and reduce tissue cell denaturalization and inflammatory cell infiltrate observed by use of light-microscopy. MgSO4 used in the early stage after irradiation could reduce the inflammatory reaction and protect brains from radiation injury.  
        关键词：Magnesium sulfate;Electron;Rats;Brain;Inflammatory reaction;ICAM-1   
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        发布时间：2023-03-30
      • CHEN Jidong,ZHANG Hong,LI Ning,WU Zhenhua
        Vol. 27, Issue 4, Pages: 239-243(2009)
        摘要：The aim of this study is to explore the DNA damage response of HeLa irradiated by 12C6+ beam and the mechanism of the p53 activation change in this response. In our present study, double strands break(DSB) of HeLa cells irradiated with 12C6+ beam were detected through neutral single cell gel electrophoresis, and AO/EB staining was used to detect the apoptosis of irradiated HeLa in 24h irradiation. Moreover, HeLa was pre-treated with caffeine (ATM and ATR inhibiting) or wormannin with certain concentrations (20μmol/L, ATM and DNA-PK inhibiting) and irradiated with 1Gy of 12C6+ beam, and the expression of p53 was detected with Western blot analysis. The results show that DSB of HeLa caused by 12C6+ beam increases with absorbed doses and decreases with the time after irradiation. The apoptosis percentage of irradiated HeLa increases with absorbed doses. It has been found that the p53 expression increases after irradiation, but has not significant increment with caffeine or wortmannin pre-treatment in cells. It can be deduced that the p53 activation is ATM-dependent, but not ATR and DNA-PK-dependent in HeLa DNA damage response induced by 12C6+ beam.  
        关键词：P53;HeLa cells;12C6+ ions;ATM   
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        发布时间：2023-03-30
      • LU Xue,LI Yuwen,FENG Jiangbin,CHEN Deqing,LIU Qingjie
        Vol. 27, Issue 4, Pages: 244-247(2009)
        摘要：Differential gene expression profiling induced by different dose levels was detected with cDNA microarray, in order to search the possible radiation biodosimeters. Lymphoblastoid cells were irradiated with 0, 0.5, 3 or 8Gy 60Co γ-rays. Total RNA was extracted in 24h after irradiation, cDNA was synthesized and labeled with biotin, and then gene expression profiling was detected with cDNA microarray. Differential expressed gene detected with microarray was certified by real-time PCR. The experimental results show that 16, 240 and 462 differential expressed genes have been detected at 0.5, 3 and 8Gy, respectively. Four genes are up-regulated and three genes are down-regulated in all the three dose groups. The dose-dependent inducible expression of TP53I3 gene has been further confirmed by real-time PCR. The expression changes of TP53I3, GDF15, CDC42EP5, S100A4, IGJ and KLF2 genes after irradiation might play a role in radiation-induced bioeffects.  
        关键词：cDNA microarray;Differential expressive gene;Lymphoblastoid cell line;60Co γ-rays   
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        发布时间：2023-03-30
      • WANG Lianfeng,CHEN Henglei,ZHANG Jun,ZENG Xianxian
        Vol. 27, Issue 4, Pages: 248-252(2009)
        摘要：In order to screen pleurotus mycelium polysaccharide high-yield strains, the comparative study was made by use of ion beam implantation and composite mutagenesis before screening.The treating mycelium pellet of pleurotus ferulae tentatively with ion beam implantation was performed at the first. Two polysaccharide high-yield strains, PFPH-1and PFPH-2, were selected using fermentation quantitative screening after auxotrophy qualitative primary screening. It has been found that the polysaccharide yield of the mutants is 551.80mg/L and 659.46mg/L respectively, which increases by 46.55% and 75.14% respectively compared to that of initial strain. Then PFPH-1and PFPH-2, as the original strain, is exposed to ultraviolet light and is suffered by additive of LiCl respectively. The results indicate that the polysaccharide yield of strains 1, 9 and 10 decreases by 27%, 38% and 37% respectively compared to that of PFPH-1 meanwhile the polysaccharide yield of strain 17 decreases by 28% compared to that of PFPH-2 after high-flux qualitative primary screening. In this study, composite mutagenesis with exposure of ultraviolet and additive of lithium chloride shows some negative effects.  
        关键词：Pleurotus polysaccharide high-yield strains;Ion beam;Composite mutagenesis   
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        发布时间：2023-03-30
      • YANG Mingcheng,ZHU Jun,ZHAO Huidong,SONG Weidong,ZHANG Hongna,LI Kunhao
        Vol. 27, Issue 4, Pages: 253-256(2009)
        摘要：The effect of radiation on appearance, color and active ingredients of products has been studied in irradiation sterilization of eye healthy care paste by use of 60Co γ-rays. The results show that irradiation is very effective on killing the microorganisms in eye healthy care paste, and the D10 of the aerobic bacterial count is determined to be 3.16 kGy. The process adopts the irradiation static stacking mode, and the suitable absorbed dose has been selected to be 7~8 kGy. It has been found that irradiation with 7~8 kGy does not affect appearance, color and active ingredients of the eye healthy care paste.  
        关键词：Eye health care paste;Irradiation sterilization;Irradiation technology   
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        发布时间：2023-03-30
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