最新刊期
      期 2 ， 2010
      • LIU Jing,CHEN Jihong,LI Wenjian
        Vol. 28, Issue 2, Pages: 65-68(2010)
        摘要：The side effect of ionizing irradiation has been paid more attention with its widely using in tumor treating and mutation breeding. In recent years, untargeted effects induced by ionizing irradiation have become a hotspot of radiobiology. Here, according to reported results, we reviewed the types (genomic instability, bystander effect and adaptive response) and mechanisms of untargeted effects of ionizing irradiation in this paper.  
        关键词：Untargeted effects;Ionizing irradiation;Free radical   
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        发布时间：2023-03-30
      • LIANG Zhengyong,ZHANG Yadong
        Vol. 28, Issue 2, Pages: 69-74(2010)
        摘要：Microwave-assisted organic synthesis, as a novel 'green’ synthesis technology, has been given more and more attention. It has been widely applied in many unit reactions, such as esterification, condensation, oxidation and reduction reaction etc. The unique and high efficiency heating effect of microwave, in many cases, could increase the reaction rate and product selectivity. Even some reactions could be taken place successfully in microwave while it was almost impossible in conventional heating. In this paper, recent progresses in the applications of microwave technique in fluorination reaction are reviewed. In addition, the problems during the course of industrialization are also discussed.  
        关键词：Microwave chemistry;Fluorination;Fluorocompounds   
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        发布时间：2023-03-30
      • DING Zengcheng,REN Jie,ZHU Liwu,WANG Yan,LIU Zuojun,TANG Fei,SHI Dan,XU Hongqing
        Vol. 28, Issue 2, Pages: 75-78(2010)
        摘要：To study the mutation method of Oxalis triangularis purpurea, the Oxalis triangularis purpurea regeneration system generated from the leaf was irradiated by 60Co γ-rays. It shows that the number of adventitious bud and root induced from the differentiation state Oxalis triangularis purpurea regeneration system are cut down with the increment of absorbed doses. After growth seedling length has not distinctly reduced while the absorbed dose is 10 Gy. And it has reduced at 25 Gy and 50 Gy of 60Co γ-rays irradiation with the differentiation state tissue of Oxalis triangularis purpurea. The mutation, such as leaf number, leaf colour and leaf shape mutation, mainly the leaf number mutation (76% of the total mutation) occur in the 60Co γ-rays irradiated group. The M2 mutation rate is 2.9%, and the main mutations are leaf number mutations.  
        关键词：γ-rays;Oxalis triangularis purpurea;Differentiation state   
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        发布时间：2023-03-30
      • LI Mingjuan,LIU Weibo,GUO Qingye
        Vol. 28, Issue 2, Pages: 79-82(2010)
        摘要：The surface phase transformation and compositions distribution of Ti based alloy irradiated by intense pulsed ion beams were investigated by X-rays diffraction (XRD) and energy dispersive spectrometry line scanning (XEDS) respectively. The results show that the un-irradiated sample mainly is composed of α-Ti and β-Ti. The diffraction peaks of surface composition disappear while its roughness increases when the target irradiated by intense pulsed ion beam with a low energy density. The minor amorphous phase has been formed when the target is irradiated by IPIB with a moderate energy density. With many pulses, the increasing of Al and O elements has been found and it means that the formation of Al2O3, which is favorable for the anti-oxidation improvement of Ti based alloy surface. With further increasing of energy density, two new phases- Al6MoTi and AlMoTi2 appeared on the irradiated surface of target.  
        关键词：Intense pulsed ion beams (IPIB);Irradiation;Ti based alloy;Phase transformation   
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        发布时间：2023-03-30
      • ZHANG Ning,YU Long
        Vol. 28, Issue 2, Pages: 83-86(2010)
        摘要：Curve equations were established to simulate the survival rate of B.trispora (−) implanted by N+ of different energy, and the survival-dose effect model was built. The results showed that the survival-dose of the three energies types fitted the GCAS curve trend with the coefficients of determination (R2) over 0.9834. With them, the "saddle shape" survival rate curve of B.trispora (−) implanted by N+ was well explained. The survival-dose effect model of B.trispora (−) implanted by low energy N+ was useful to the B.trispora (-) mutation breeding work, and was significant for other fungi as reference.  
        关键词：Ions implantation;Survival-Dose Effect;Model;B.trispora (−)   
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        发布时间：2023-03-30
      • LIU Yajian,TAN Yuanyuan,ZHANG Yuehong,FU Junjie
        Vol. 28, Issue 2, Pages: 87-90(2010)
        摘要：60Co γ-rays irradiation method was applied to prepare nano-particle copper in aqueous solution. The nano-particles with different sizes were prepared by use of irradiation with different absorbed doses. The samples were analyzed with X-rays powder diffraction (XRD), electron microscopy (TEM) and laser scattering particle size distribution analysis (LSPSDA), which were used to determine the constitution, shape and size distribution of the particle, respectively. It has been found that average size of copper nano-particle decreases with the increment of radiation absorbed dose. The sample is a mixture of cuprous oxide and pure copper when absorbed dose is low. And pure copper nano-particles can be obtained at 400 kGy. The morphology of the nano-copper particle can not be affected by absorbed dose and most particles are spherical.  
        关键词：γ-irradiation;Nano-particle copper;Preparation   
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        发布时间：2023-03-30
      • Ismayil KIRAM,Mamatjan YIMIT,Ismayil NURULLA,MI Hongyu
        Vol. 28, Issue 2, Pages: 91-97(2010)
        摘要：In order to synthesize of water super-absorbent resin, an interpenetrating network (IPN) salt-resistant polymer was prepared with starch, acrylic acid (AA) and poly (vinyl alcohol) (PVA) by use of UV light from a high-pressured ultraviolet mercury lamp without using any cross-linker and initiator. The influences of neutralization degree, reaction time, the content of starch and PVA on the water absorbent performance of sample were studied. The absorbent perfomance of the resin polymerized under the optimum condition is as follows, the distilled water absorbency is 2235 g/g and salty water (0.9% NaCl) absorbency is 148 g/g.  
        关键词：UV polymerization;Interpenetrating network;Super absorbent resin;Grafting copolymerization   
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        发布时间：2023-03-30
      • LI Qinghua,YUE Ling,YANG Shuqin,CHEN Meiling,ZHU Nankang,LI Xinnian,YANG Zhanshan
        Vol. 28, Issue 2, Pages: 98-102(2010)
        摘要：The effects of neutron radiation shielding were studied by use of a new hydrogel. A simple PVA/PEO hydrogel and the hydrogel containing metal ions were prepared by physical cross-linking method. The effects of hydrogels to 2.45 MeV neutron radiation shielding were theoretically analyzed by SHIELD procedure which is based on Monte Carlo simulation to calculate the neutron transport situation in different hydrogels. The flux counts of 2.45MeV neutron through different thicknesses of hydrogels were measured by a BF3 neutron detector using a K-400 type high-voltage multiplier. The Monte Carlo simulation results show that neutron flux and energy decrease gradually with increment of hydrogel thickness. Compared with simple group, the neutron flux and energy through the same thickness of group containing metal ions decrease more obviously. The results measured by BF3 neutron detector show that the percentage change of neutron flux count through 6—10 cm thickness of hydrogel containing metal ions to 2.45 MeV neutron increase significantly compared to simple hydrogel, and it comes to 61.3% at 10 cm thickness of the hydrogel, and its radiation shielding linear equation is y=-4.5103x +86.229. It can be concluded that PVA / PEO hydrogel has a better neutron irradiation shielding effect, especially for the PVA / PEO hydrogel containing metal ions.  
        关键词：Hydrogel;Metal ions;Neutron radiation;Shielding effect;SHIELD procedure   
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        发布时间：2023-03-30
      • WU Shanshan,LIU Chao,ZHANG Buchang,CAO Lei,WANG Xiaoming
        Vol. 28, Issue 2, Pages: 103-106(2010)
        摘要：Saccharopolyspora erythraea 963 with antibacterial activity was a mutant strain which obtained by 60Co γ-rays irradiation on Saccharopolyspora erythraea M. In this experiment, Saccharopolyspora erythraea 963 was also radiated by 60Co γ-rays to study repeated mutagenic effects. The results indicate that the lethal effect of strain 963 is lower than that of starting strain M in the same absorbed doses of irradiation. After the repeated mutagenesis, the rate of morphological variation of strains was up to 24.1‰ and equal to 4.8 times as that of starting strain M. The inhibitory effect of positively mutated strain increased by 40% and the rate of positive mutation was 2.01‰ and rose by 20.36% compared with that of the strain 963. The method of repeated mutagenesis is helpful in improving screening efficiency of mutation breeding as the morphological variation of mutated strain and positive mutation can be obtained.  
        关键词：60Co γ-rays;Saccharopolyspora erythraea;Mutagenic effect   
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        发布时间：2023-03-30
      • SHAO Na,WEI Gongyuan,GE Xiaoguang,NIE Min
        Vol. 28, Issue 2, Pages: 107-113(2010)
        摘要：S-adenosyl-L-methionine (SAM) and glutathione (GSH) are both applied extensively in the fields of medicine, cosmetics and health maintenance for their biological functions multiplicity in vivo. In this paper, a complex mutagenesis was carried out to attenuate the contradiction so as to obtain a positive mutant which can produce SAM and GSH effectively. The parent strain Candida utilis SZU 07-01 was treated by two turns of UV and once γ-rays irradiation for complex mutagenesis, following by cultivating and screening on the selective medium (YPD containing 1 g/L L-Met, 0.5 g/L ethionine and 2 g/L NaHSO3) for a positive mutant C. utilis SUGS-01. The proliferation of C. utilis SUGS-01 was then carried out in a flask, and the productions of S-adenosyl-L-methionine and glutathione were 324.0 mg/L and 220.5 mg/L, respectively, which were 131.4% and 26.0% higher than those of C. utilis SZU 07-01. In addition, C. utilis SUGS-01 shows high genetic stability even after 10 generations of cultivation, which indicates that the mutant C. utilis SUGS-01 obtained in this study has stable capacity for high co-production of SAM and GSH, and can be used as industrial strain in the near future.  
        关键词：Candida utilis;Co-production;S-adenosyl-L-methionine;glutathione;UV and γ-rays mutagenesis   
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        发布时间：2023-03-30
      • YANG Kang,ZHU Jiayun,LI Junhong,DING Nan,HU Wentao,HE Jinpeng,SU Fengtao,LI Sha
        Vol. 28, Issue 2, Pages: 114-118(2010)
        摘要：To investigate the relationship between DNA-PKcs and genome instability & hyper-radiosensitivity, human glioma cell lines M059K and M059J, as a model expressing wild-type DNA-PKcs and a model defective in DNA- PKcs activity, were exposed to low doses of X-rays. Cells survival fractions were assessed by colony-forming assay and Cytochalasin-B micronucleus assay was employed to detect the genomic instability happening in each single irradiated colony. It has been found that as the post-incubation time increased, M059K cells expressing wild-type DNA-PKcs exhibited low-dose hyper-radiosensitivity and showed a similar genomic instability after 0.2 Gy and 0.6 Gy irradiations, but the M059J cells lacking in DNA-PKcs didn’t present low-dose hyper-radiosensitivity and showed a higher genomic instability of 0.6 Gy than that of 0.2 Gy. The results indicate that DNA-PKcs may act as one of the key factors that lead to low-dose hyper-radiosensitivity.  
        关键词：Genomic instability;Hyper-radiosensitivity;X-rays;DNA-PKcs   
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        发布时间：2023-03-30
      • LIU Jinting,DANG Bingrong,LI Wenjian,JING Xigang,WEI Wei,WANG Zhuanzi,DING Nan,GAO Qingxiang
        Vol. 28, Issue 2, Pages: 119-123(2010)
        摘要：In this paper, the relationship between cell survival fraction and telomerase activity was investigated with the human hepatocellular cells HL-7702 and carcinoma cells SMMC-7721 irradiated by X-rays. The colony formation assay and TRAP (Telomeric repeat amplification protocol) method were used to determine the cell survival fraction and telomerase activity respectively. To both of HL-7702 and SMMC-7721, the telomerase activity was increased in dose-dependent. However, at 1—3 Gy, the telomerase activity was lower than that of control in SMMC-7721, and it was a little higher than that of control when irradiated at 4 Gy. At 1—4 Gy, the variation trend of cell survive and that of telomerase activity were identical.  
        关键词：X-rays;Cell survival rate;Telomease activity;SMMC-7721;HL-7702   
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        发布时间：2023-03-30
      • LIU Ping,TU Yu
        Vol. 28, Issue 2, Pages: 124-127(2010)
        摘要：In order to investigate the effect of ionizing radiation on neural stem cells differentiation, we cultured neural stem cells of newborn rat in serum-free media containing EGF or bFGF .The neural stem cells were divided into 4 groups, which were irradiated by γ-rays with doses of 0, 0.5, 1, and 2 Gy. The irradiated cells were cultured under the same condition for 7 days, and the nestin content of neural stem cell was detected by immunofluorescence. The same method was carried out with irradiated cells in the culture medium after removing EGF, bFGF for 7 days, NSE and GFAP expression content and nestin were also detected by immunofluorescence. It has been found that the irradiated neural stem cells can express less nestin and differentiate more neurons compared to that of control group. Results show that ionizing radiation can induce the differentiation of the neural stem cells and make the neural stem cells differentiate more neuron.  
        关键词：Neural stem cell;Ionizing radiation;Differentiation   
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        发布时间：2023-03-30
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