最新刊期
      期 3 ， 2010
      • LI Weiming,HA Yiming,ZHANG Yanli
        Vol. 28, Issue 3, Pages: 129-133(2010)
        摘要：In the article, we report our progresses in using electron spin resonance (ESR) technique to identify irradiated food containing cellulose. Several issues that might be concerned in the ESR application are discussed, and suggestions are given for extending the ESR application.  
        关键词：Electron spin resonance (ESR);Cellulose;Irradiated food;Detection   
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        发布时间：2023-03-30
      • LIU Jiangong,GUO Wanlong,ZHANG Shuxian,DUAN Zhikai
        Vol. 28, Issue 3, Pages: 134-137(2010)
        摘要：At present, Cytokine therapy is the main strategy capable of preventing and reducing the acute radiation syndrome (ARS). With the problem of difficult match and severe graft versus host disease, haemopoietic stem cells can be used to find some effective approaches to treat acute radiation damage. Mesenchymal stem cells are of great therapeutic potential due to their particular characteristics including secretion of hematopoietic cytokine, reconstruction hemopoietic microenvironment, poor-immunogenicity, ease of reception ectogenic gene transfection and expression. This paper is to summarize the studies of biological characteristics of MSC and its application prospects in urgent radiation damage.  
        关键词：Acute radiation injury;Mesenchymal stem cells;Cytokine   
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        发布时间：2023-03-30
      • CHEN Hui,HE Hui,YE Guoan,FU Haiying,WU Guozhong
        Vol. 28, Issue 3, Pages: 138-143(2010)
        摘要：Pulse radiolysis of aqueous solution containing N-Dimethylhyroxylamine was studied under different conditions. The transient species with characteristic maximum absorption were identified and the build-up/decay time-resolved curves were recorded. The rate constant for reaction of ·OH radical and N, N-Dimethylhyroxylamine to form (CH3)2NO· radical was determined to be (4.5±0.3)×109 mol-1·L·s-1 meanwhile the rate constant for reaction of eaq- and N, N-Dimethylhyroxylamine to form (CH3)2N· radical was determined to be (1.3±0.04)×109 mol-1·L·s-1.  
        关键词：N;N-Dimethylhyroxylamine;Pulse radiolysis;Transient absorption spectrum;Free radical   
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        发布时间：2023-03-30
      • Mamatjan YIMIT,Rukiya MATSIDIK,Ismayil KIRAM,Ismayil NURULLA
        Vol. 28, Issue 3, Pages: 144-149(2010)
        摘要：In order to increase the hydrophility of polypropylene (PP), acrylic acid(AA) was grafted on to the surface of PP powder by means of photo-initiated solid phase grafting reaction with the photo-sensitizer of benzophenone in the reaction equipment of UV polymerization. The effects of AA content, amount of initiator, irradiation time, and swelling time on grafting degree were systematically studied. The result shows that in the processing of AA grafting reaction the highest value, 5.2%, of grafting degree can be obtained when the content of AA is 1 mL, amount of initiator is 0.15 g, irradiation time is 30 min and swelling time is 12 h. With the grafted sample the dye ability is 43.4%, tensile strength drops 1.37 MPa of the grafted PP, but breaking extension degree increases to 396.3% from 358.3%. This indicates that the effect of moderate grafting of acrylic acid on the mechanical performance of PP is not obvious.  
        关键词：Polypropylene;Acrylic acid;Mechanics performance;Surface photo-grafting   
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        发布时间：2023-03-30
      • LIU Bin,XIONG Guangquan,XIONG Shanbai,CHENG Wei,CHEN Yuxia,LIAO Tao,LI Xin,LIN Ruotai
        Vol. 28, Issue 3, Pages: 150-155(2010)
        摘要：The degradation of five kinds of fishery drugs in aqueous solution irradiated by 60Co γ-rays at the absorbed dose of 0—10 kGy was investigated. The effects of acid/base, degassing and temperature on the degradation of fishery drugs were studied. The results show that fishery drugs can be degraded significantly with 60Co γ-rays irradiation, and the higher absorbed dose the higher degradation efficiency. The degradation percentage increases with the decrement of initial concentration of fishery drugs. More than 90% degradation of five fishery drugs has been achieved at absorbed dose of 8 kGy. The effects of acid/base and degassing environments on the degradation of fishery drugs are different. The lower temperature can inhibit remarkably the degradation of fishery drugs in aqueous solution.  
        关键词：γ-rays irradiation;Fishery drugs;Degradation   
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        发布时间：2023-03-30
      • LI Shurong,LIN Qiong,MAO Tao,GAO Meixu,WANG Zhidong,ZHU Jie,ZHOU Hongjie,LI Qingpeng,FAN Pei,WANG Feng
        Vol. 28, Issue 3, Pages: 156-160(2010)
        摘要：The shrimp were irradiated by 60Co γ rays at the dose of 1, 3, 5, and 7 kGy. The shrimp was firstly Soxhlet extracted and then purification of the shrimp oil was carried out by solid-phase extraction column to remove the fat and pigment. The hydrocarbons contained in the shrimp were determined by GC-MS among irradiated and unirradiated samples. The results show that C17:1 and C16:1 produces were the new types of hydrocarbons in irradiated shrimp with recovery of 78.2% and 88.06%, respectively. Content of C17:1 and C16:1 increased with the increase of the absorbed dose.  
        关键词：Irradiation;Shrimp;hydrocarbons;GC-MS   
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        发布时间：2023-03-30
      • YU Lixia,LI Wenjian,CHEN Xuejun,CHEN Jin,XIE Hongmei
        Vol. 28, Issue 3, Pages: 161-165(2010)
        摘要：In order to study mutagenic effects of different heavy ions irradiation on maize inbred lines, corn seeds of Zheng58, Lu9801, Jinxiang4C-1, CSR24001, 308 and 478 were irradiated with 12C6+ and 36Ar18+ ions. The experimental results showed that the germination rate and planting percent were different after irradiation. The wettish seeds had higher sensibility to heavy ion irradiation. The leaf type of the plant appeared visible changes in M1 generation. In M2 generation, great changes had taken place in economic traits, many of which are beneficial mutation. Some beneficia1 mutation could be stably inherited in M3 generation. From the above, it can be predicted that heavy ions irradiation is an effective means of genetic improvement of maize.  
        关键词：Heavy ions irradiation;Maize inbred lines;mutagenic effects   
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        发布时间：2023-03-30
      • CHEN Tingting,LIAN Lixia,MU Ying,YANG Zhanshan
        Vol. 28, Issue 3, Pages: 166-171(2010)
        摘要：In this paper the effects of pprI gene from Deinococcus radiodurans transfered by electroporation in vivo on acute radiation injury of a mammalian had been investigated and clarified. After injecting the pCMV-HA plasmid into the muscle of mice exposed to γ-rays irradiation, pprI gene was transferred into the cell of mice with electroporation technology in vivo. The deaths of mice, blood cell count and the apoptotic rates of bone marrow cells, spleen cells and thymocyte cells on the 1st, 7th, 14th, 28th, and 35th day after irradiation were investigated. The results show 6 Gy γ-rays can cause acute lethal radiation injury of mice and the death rate of transfer pCMV-HA-pprI group (1/10) is obvious lower than that of radiation group(4/10). Compared with the radiation irradiated group and transfer pCMV-HA group, the leucocyte number and the erythrocyte number in peripheral blood of the mice transferred with pCMV-HA-pprI group is significantly higher on the 7th day after irradiation (p<0.05) meanwhile the platelet number is obvious higher on the 7th day and the 14th day (p<0.05). On the other hand, the lymphocyte ratio get right on the 35th day, the apoptotic rate of spleen cells is significantly lower on the 7th, 14th, and 28th day (p<0.05), the apoptotic rate of thymocytes is significantly lower on the 1st, 7th, 14th and 35th day (p <0.05), the apoptotic rate of bone marrow cells is significantly lower on the 1st, 7th, 14th and 28th day (p<0.05), the apoptotic rates of thymocytes and bone marrow cells are return to normal level on the 28th day after irradiation had also been observed in the group transferred with pCMV-HA-pprI. The result indicates that the pprI gene of Deinococcus radiodurans transfered by electroporation in vivo can prevent and cure the acute radiation injure for animals apparently.  
        关键词：Deinococcus radiodurans;pprI;Gene therapy;In vivo electroporation;Radiation injury   
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        发布时间：2023-03-30
      • LU Minxia,NIE jihua,ZHU Jianquan,QIAN Cheng,WANG Guohai,TONG Jian
        Vol. 28, Issue 3, Pages: 172-176(2010)
        摘要：To explore the biological effects of microwave combined with γ-rays on apoptosis, mitochondrion membrane potential(MMP), Ca2+and Ca2+-Mg2+-ATPase in rat cultured gliacytes, primarily cultured gliacytes were randomly divided into the control group(C), the microwave group(M), the γ-rays group(I) and the combined group (IM). The M and IM groups were exposed to 4 mW/cm2 microwave 2h/d for 3 days. The I and IM groups were given 5 Gy 60Co γ-rays on the forth day. Ca2+-Mg2+-ATPase was detected by kit. Apoptosis, MMP and Ca2+ were detected by flow cytometry(FCM). The results show that the ratio of apoptosis of the I and IM groups were significantly increased, and the contents of Ca2+ in the cytoplasm of M, I and IM group cells were all significantly higher meanwhile Ca2+-Mg2+-ATPase activity was lower than that of the control group; but MMP had no obvious changes in the four groups. Under the experimental conditions, the microwave radiation did not induce evident changes in MMP. However, the content of Ca2+ in the cytoplasm increased significantly and Ca2+-Mg2+-ATPase decreased obviously. No synergic effects were observed in the treatment with microwave before exposure to γ-rays.  
        关键词：Microwave;γ-rays;Apoptosis;Cultured gliacytes;MMP;Ca2+   
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        发布时间：2023-03-30
      • PAN Luyu,CAO Jianping,CHEN Guanglie,JI Rong,FENG Yang,ZHOU Yuanyuan,PENG Xiaomei,CUI Dan,ZHU Wei,FAN Saijun
        Vol. 28, Issue 3, Pages: 177-180(2010)
        摘要：In order to compare the radiation sensitizing effect of artemisinin and artemether to human nasopharyngeal carcinoma CNE-1 cell, the human nasopharyngeal carcinoma CNE-1 cell in index vegetal period was selected as experimental sample, and the cells clone-forming method was used to test growth inhibition of artemisininand and artemether to the CNE-1 cell, to estimate the difference of inhibitory effect between the two drugs, and to determine suitable drug concentration. The experimental samples were divided into control group, simple drug group, irradiated group and union treatment group with human nasopharyngeal carcinoma cells. The simple drug and union treatment group were also divided into artemisinin and artemether group, respectively. The exposed doses were set up as 0, 2, 4, 6, and 8 Gy. The cell survival curve was fitted by an multi-target one-shot mathematic model and taking the radiation sensitizing enhancement ratio/SER (SER) equal to the dosage ratio of irradiated group and union treatment group when the same biological effect was achieved. It has been found that both drugs can enhance the inhibitory effect with the increment of medicine concentration. The radiation sensitizing enhancement ratio/SER (SER) has been determined to be 1.272 and 1.481for artemisinin and artemether, respectively, which suggests that artemether has stronger radiation sensitizing effect compared with artemisinin in human nasopharyngeal carcinoma CNE-1 cells.  
        关键词：CNE-1 cell line;Radiosensitive enhancement effect;Artemisinin;Artemether   
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        发布时间：2023-03-30
      • PAN Yanling,HONG Chengjiao,ZHANG Baoguo
        Vol. 28, Issue 3, Pages: 181-184(2010)
        摘要：In order to investigate the radio-sensitizing effect of 17-AAG, an inhibitor of Heat Shock Protein 90, on human Uterine Cervix Cancer HeLa and V79 cells, Clonogenic assay was used to observe the cell survival rate. The results show that 17-AAG can decrease obviously (p<0.01) the clonogenic survival rate of HeLa cells irradiated by X-rays but not the V79 cells (p>0.05). This indicates that 17-AAG may enhance the radio-sensitivity of the HeLa cell line and has no effect on the V79 cell line.  
        关键词：17-AAG;Cell Radio-sensitivity;HeLa cell;V79 Cell   
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        发布时间：2023-03-30
      • QIN Fenju,NIE Jihua,CAO Yi,LI Jianxiang,TONG Jian
        Vol. 28, Issue 3, Pages: 185-189(2010)
        摘要：In order to observe the effects computer radiation(CR) on learning and memory ability as well as cerebra neuron transmitter of mice, the 32 male Kunming mice were randomly divided into four groups as one control group and three CR groups irradiated for 6 h/d, 12 h/d, and 18 h/d, respectively. The three CR groups were settled 20cm before the operating computer (0.9 V/m) for 30 days consecutively. And the control group was placed in the environment without computer radiation (0 V/m). The learning and memory ability of the mice were measured by Y-type maze test at thirty-first day, then the activity of acetylcholinesterase (AchE), nitric oxide synthase (NOS) and glutamine synthetase (GS) were measured as well as the content of acetylcholine(Ach), nitric oxide(NO) and Glutamate (Glu). The results show that the content of Ach in cerebra decreased, but the content of NO and Glu were just the opposite; the activities of AchE and NOS were enhanced, but the activity of GS was just the opposite. It is revealed that the computer radiation can reduce the learning and memory ability of mice, which may involve the changes of cerebra neuron transmitter.  
        关键词：Computer radiation;Learning-memory;Acetylcholine;Acetylcholinesterase;Nitric oxide;Nitric oxide synthetase;Glutamate;Glutamine synthetase   
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        发布时间：2023-03-30
      • YU Lei,SUN Shilong,FANG Fang,LIU Yongzhe,GONG Hongwei,SHI Lei,JU Guizhi
        Vol. 28, Issue 3, Pages: 190-192(2010)
        摘要：In order to investigate the relation between kras gene and thymic lymphomas of mice induced by X-rays, we made the thymic lymphomas model of BALB/c mice exposed to X-rays, so as to extracte total RNA and synthesize cDNA and total protein from both of thymic lymphomas and normal thymus tissue. The mRNA and protein expression of kras gene in thymic lymphomas and normal thymus were detected by QRT-PCR and Western blot, respectively. The results show that mRNA expression of kras gene in thymic lymphomas (2.66 ± 1.51) is significantly higher (p<0.05) than that of normal thymus tissue (0.93±0.30); and protein expression of kras gene in thymic lymphomas is higher than that of normal thymus tissue. The results suggest that kras gene might be relative to thymic lymphomas induced by X-rays, and it might be a susceptibility gene to radiation carcinogenesis.  
        关键词：Thymic lymphomas;Kras genes;Ionizing radiation;Quantitative real-time PCR   
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        发布时间：2023-03-30
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