最新刊期
      期 2 ， 2011
      • ZHANG Deqin,LIU Qingjie
        Vol. 29, Issue 2, Pages: 65-69(2011)
        摘要：Radiation biodosimeter plays a vitally important role in estimating the absorbed doses of radiation victims and in the research on radiation biological effects. The "golden" biodosimeter, chromosome aberration analysis, is not suit for fast and high-throughout dose estimation. Developing a new fast and simple biodosimeter became the hotspot in radiation biodosimetry. Some studies showed that ionizing radiation could induce the gene expression changes (GEC) in the network of transcriptional regulation pathway, and GEC had the potential to be a biodosimeter. Many studies focused on p53 pathway-associated genes, including mdm2, Cyclin G, CDKN1A, GADD45, PIG3, Fhl2, ISG20L1, PCNA and GDF15.  
        关键词：Radiation biodosimeter;p53 pathway;Gene expression   
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        发布时间：2023-03-27
      • ZHENG Chun,SONG Lingli,AI Zihui
        Vol. 29, Issue 2, Pages: 70-76(2011)
        摘要：Radiation biology research in China during the past ten years are reviewed. It should be noticed that radiation-biology should focus on microdosimetry, microbeam application, and radiation biological mechanism.  
        关键词：Ionizing radiation;Microdosimetry;DNA;Double-strand breaks   
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        发布时间：2023-03-27
      • MIN Rui
        Vol. 29, Issue 2, Pages: 77-83(2011)
        摘要：A large number of epidemiological data have shown that, the incidence of radiation-induced cancer in a certain range of radiation dose is linearly related with exposure doses. And the dose ranges that people received during performing some of radiation therapy and medical radiological diagnosis are highly consistent with dose ranges where an increased risk of cancer groups was found by epidemiological investigation. With an increasing application of radioactive diagnostic and therapic equipments in disease diagnosis, treatment and healthy checks the potential health hazards due to radiation among a large number of patients and individuals performing healthy check should erect more concern. In risk assessment caused by medical radiation following the LNT model assumes at present still has a scientific and practical significance to reduce hazards.  
        关键词：Radiation risk;Epidemiologic evaluation;Medical exposure;Linear non-threshold model   
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        发布时间：2023-03-27
      • LIU Jianwei,LIN Lin,NIE Jun,HE Yong
        Vol. 29, Issue 2, Pages: 84-88(2011)
        摘要：The photo-physical properties of the water-soluble substituted azobenzene monomer, 4-[4-(acryloyl)phenylazo]benzene sulfonic acid (APABS), have been studied, and the effects of the solvent on its photo-isomerization performance were investigated. With N, N′-methylenebis acrylamide (MBAA) as cross-linking agents, the copolymer hydrogels of APABS and HEA were prepared through photo-polymerization method. The effects of the polymerization temperature on the interior morphology of hydrogels have been examined by scanning electron microscopy (SEM).  
        关键词：Azo dye;Photo-isomerization;Photo-polymerization;Hydrogels   
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        发布时间：2023-03-27
      • LEI Qing,HUANG Min,DENG Wenmin,WU Ling,WANG Yan,GAO Peng,DU Xiaoying,XIE Yan
        Vol. 29, Issue 2, Pages: 89-92(2011)
        摘要：To explore the sterilization dose setting method for medical products, the irradiation sterilization dose for medical products was set according to the method 2A of international standard ISO11137 Part 2. The verification dose for medical gauze pad by was set by incremental dose method and with a series of formula. Then the sterilization dose for an SAL of 10-6 was established by completing the verification dose experiment. The final sterilization dose for medical gauze pad was decided to be 29.7 kGy. This study gives reference for the setting irradiation sterilization dose for medical products which need to adopt the method 2A of international standard ISO11137 Part 2.  
        关键词：Medical gauze pad;Irradiation sterilization;Incremental dosage;Verification dose   
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        发布时间：2023-03-27
      • LI Mingjuan,WANG Weiwei,CHEN Shiwei,CHENG Ying,MIN Rui
        Vol. 29, Issue 2, Pages: 93-98(2011)
        摘要：In this paper the expression changes of genes involved in DNA repair induced by radiation at different doses and post-irradaition time points has been investigated and the possibility of combining some genes as biomarkers for bio-dosimeter has been evaluated. The whole body of experimental mice was irradiated with 0, 2, 4, 6 and 8 Gy 137Cs γ-rays at 0.80 Gy/min and 1.60 Gy/min, respectively. Peripheral blood was collected at immediate, 4, 8, 12, 24 and 48 h after irradiation and RNA was extracted by AxyPrep Kit. The mRNA transcriptional expression levels of ATM, CDKN1A, DDB2 and GADD45A were detected by real-time quantitative polymerase chain reaction (RT-PCR). Expression changes of mRNA were compared with that of unirradiated control group at the dose rate of 0.8 Gy/min. A series of dose-and time-response of the changes were figured out. A heavy change in magnitude of genes transcription induced by irradiation at higher exposure dose rate could be found. The information from gene expression analysis as a greatly potential biomarkers may be used to indicate exposure doses at different post irradiation time points in a certain dose range.  
        关键词：Ionizing radiation;Biodosimeter;Gene expression;Real-time quantitative polymerase chain reaction (RT-PCR)   
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        发布时间：2023-03-27
      • ZHAO Lin,XIE Yanzhao,PAN Jianru,LIU Shutao
        Vol. 29, Issue 2, Pages: 99-103(2011)
        摘要：By means of trans-dermal permeation on rat skin in vitro, the effect of low-frequency ultrasound (40 kHz) on trans-dermal permeation of (Protein transduction domain-superoxide dismutase, PTD-SOD) has been investigated. The results show that the enhancement ratios of ultrasound and Azone are 3.40 and 2.52, respectively, when the trans-dermal permeation time is 8 h. In the presence of low-frequency ultrasound, when the initial pH value of drug solution is 6.0, the activity of PTD-SOD in receiver cell reaches 1598.00 U, the maximum value. Under the action of low-frequency ultrasound, the trans-dermal permeation of drug varies as a function of the initial PTD-SOD concentration according with a Fick-type scattering law, and the coefficient of permeation is 0.011. These results demonstrate that Low- frequency ultrasound can significantly improve trans-dermal transport of PTD-SOD, and the effect is superior to Azone. Both of the initial pH value of drug solution and the initial PTD-SOD concentration can affect the permeation rate of the PTD-SOD. And the best initial pH value and drug concentration is 6.0 and 15.00kU·mL-1, respectively.  
        关键词：Trans-dermal permeation;Low-frequency ultrasound;Protein transduction domain (PTD);Superox-ide dismutase (SOD)   
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        发布时间：2023-03-27
      • WU Xiaofen,HONG Chengjiao,GUO Wenxiu,PAN Yanling,ZHANG Baoguo
        Vol. 29, Issue 2, Pages: 104-108(2011)
        摘要：In order to investigate the mechanism of Quercetin on radio-sensitivity of human Uterine Cervix Cancer HeLa cells, HeLa cells were cultured in different concentrations of Quercetin and different doses of irradiation. The clonogenic assay was used to observe the cell servival rate. The repair of DNA double-strand breaks and effect of Quercetin combination of radiation on the cell cycle were detected by flow cytometry. The results show that the radio-sensitivity of Quercetin on HeLa cells was obvious and the unrepaired DSBs after irradiation increased, but did not decrease G2 / M cell cycle arrest. From this it can be inferred that the effect on HeLa cell radio-sensitivity may be related to the inhibition of the repair of DNA double-strand breaks induced by Quercetin, but it dose not reveal a significant relation with the cell cycle and G2/M arrest.  
        关键词：Quercetin;HeLa cell;Radio-sensitivity;The DNA damage repair;Tumor therapy   
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        发布时间：2023-03-27
      • YANG Bing,ZHOU Hui,ZHONG Leilei,YANG Fujun,ZHANG Xiaodong,LIU Zheng,ZHAO Ji,FAN Feiyue,HAN Ying,SUN Yuanming
        Vol. 29, Issue 2, Pages: 109-112(2011)
        摘要：In order to investigate the changes of the Notch signaling pathway in osteoclast after radiation the mechanism radiation-induced bone loss, the gene expression of CBFα1, the important signal molecular in Notch signaling pathway was measured by real-time PCR, respectively. The osteoclast was obtained by RANKL-dependent differentiation in the RAW264.7 cell line. It was found that the 2 Gy 137Cs γ-rays enhanced the CBFα1 expression in RANKL-induced osteoclasts. Taken together, the higher expression of CBFα1 might reinforce the effect of promoting osteoclastogenesis by Notch signaling pathway, increase both of the number and activity of osteoclasts while the bone loss, osteoporosis and fracture occur.  
        关键词：Receptor activator of nuclear factor-b ligand (RANKL);CBFα1;Radiation;RAW246.7   
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        发布时间：2023-03-27
      • GAO Ling,LI Fengsheng,CHEN Xiaohua,LIU Qingjie
        Vol. 29, Issue 2, Pages: 113-116(2011)
        摘要：This study is to investigate the effect of ionizing radiation on the activity and localization of STAT3 in gastric adenocarcinoma cells. After irradiated with 60Co γ-rays, the content and activity of STAT3 in gastric adenocarcinoma BGC-823 cells were detected by Western blot. The content of STAT3 in nucleus of BGC-823 was further detected by Western blot after the total protein in nucleus of irradiated BGC-823 was extracted. Finally, the distribution of p-STAT3(Y705) in BGC-823 treated with or without irradiation was examined by immunofluorescence staining. The results showed that the level of p-STAT3(Y705) in BGC-823 cells decreased significantly after 60Co γ-rays irradiation, and the content of STAT3 in BGC-823 cells nuclei also decreased after irradiation. As predicted, the p-STAT3 (Y705) localized in nucleus of BGC-823 cells also decreased significantly. 60Co γ-rays irradiation inhibited the phosphorylation of p-STAT3 (Y705) and further suppress the nucleus localization of STAT3, which might be involved in radiation-induced cell proliferation inhibition and apoptosis of gastric adenocarcinoma cells.  
        关键词：Gastric adenocarcinoma cell;Ionizing radiation;STAT3;Nucleus localization   
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        发布时间：2023-03-27
      • ZHANG Yongqin,ZHOU Hui,CHEN Jie,YANG Zhanshan
        Vol. 29, Issue 2, Pages: 117-122(2011)
        摘要：In order to express and purify PprI protein from D. radiodurans R1 in E. coli, the full length of pprI gene was gained by PCR amplification using pCMV-HA-pprI as a template. The gene segment was inserted into vector pET-28a after digested by two restriction endonucleases Nco I and EcoR I. Then the recombinant vector pET-28a-His-pprI was transfected into E. coli BL21 (DE3) RP. The PprI protein expression was induced by IPTG and the fusion protein was confirmed by SDS-PAGE and Western blotting. The expressive conditions of the protein such as E. coli’ A600, concentration of IPTG, time and temperature of culture, were optimized. Finally the fusion protein was purified by Ni-NTA His Bind Resins and molecule boult. The experimental results show the fusion protein confirmed by Western blotting is 6×His-PprI and its molecular weight is 37 kDa. The ladders of PprI protein at molecular weight 37 kDa were different due to difference of the PprI protein expression conditions in E. coli. The PprI protein exists both in supernatant and precipitation. The concentration of purified protein is about 0.15 mg/mL which was measured by BCA method. It is concluded that the recombinant plasmid pET-28a-His-pprI is constructed and the PprI fusion protein is expressed and purified. The results lay a solid foundation for studying the radio-resistance and immunity of PprI protein.  
        关键词：D.radiodurans R1;PprI gene;Prokaryotic recombinant vector;Protein expression;Protein purification   
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        发布时间：2023-03-27
      • WU Yinxia,LIU Dongfang,LIU Yongbiao,XU Dongmei,YAO Side,SHENG Kanglong
        Vol. 29, Issue 2, Pages: 123-128(2011)
        摘要：As a neotype nonviral vector, (Polyethylenimine, PEI) has been studied in gene transfection experiment. This study was investigated the growth inhibition and radio-sensitizing effect of E1A gene transfected by PEI on human colon carcinoma cell in vitro. The PSV-E1A recombinant plasmid, which was designed for high-level expression of E1A gene in a variety of eukaryotic cell lines, was transfected into SW480 cells by PEI. The transfection was confirmed by RT-PCR and G418 was used to get colon carcinoma cells stably expressed E1A gene. The cell growth curve were investigated to observe the growth inhibition induced by E1A gene. The redistributions of cell cycle were analyzed by flow cytometry. Cells before and after transfection were treated with irradiation, then the changes of radiation-sensitivity were tested by MTT assay after 24 h meanwhile the expression of HER-2 gene in SW480 cells before and after transfection was detected by western-blot. As results, (1) the colon carcinoma cells expressed E1A gene was confirmed by G418. (2)The result of RT-PCR demonstrated that PEI could transfect plasmid psv-E1A and the cells could stably express E1A gene.(3)Flow cytometry revealed that E1A gene transfected into human colon carcinoma cell could induce S stage suppression (p<0.001) and G2/M stage arrest (p<0.001). (4)Compared with the Non-transfected cells, the E1A-transfected cells (SW480-E1A cells) grew slowly observed by MTT assay which was used to get the absorbance of SW480 cell and SW480-E1A cell. (5)The radiation-sensitivity of SW480 cells transfected with E1A gene was up -regulated obviously (p<0.001). (6) The E1A gene obviously down-regulated HER-2 protein expression in colon carcinoma cells. Anyway, PEI can transfect plasmid psv-E1A gene which can significantly inhibit the growth rate of SW480 cell. Moreover, it also obviously enhanced the cell sensitivity to irradiation.  
        关键词：E1A gene;Gene delivery;Colon carcinoma cell;Polyethylenimine;Radiotherapy   
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        发布时间：2023-03-27
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