最新刊期
      期 4 ， 2011
      • WANG Zhuanzi,LI Wenjian,WANG Biqian
        Vol. 29, Issue 4, Pages: 193-196(2011)
        摘要：The application of heavy-ions in radiation oncology and the development of human space exploration have greatly contributed to the heavy-ions radiobiology. Chromosomal aberration is a typical biomarker of radiation damage. The progresses obtained in mammalian cells chromosome aberrations induced by heavy-ions have been reviewed in this paper.  
        关键词：Heavy-ions;Chromosome aberrations;Relative biological effectiveness   
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        发布时间：2023-03-27
      • LI Feng,LONG Linmei,LIANG Zhongqin
        Vol. 29, Issue 4, Pages: 197-201(2011)
        摘要：Radiotherapy is one of the most important methods in the combination therapy against glioma, but resistance of the malignant glioma to radiation limits its clinical contribution. Therefore, it makes great sense to study new mechanisms of radio-resistance. Recent studies show that there is a relationship between EGFR and autophagy in human glioma radiotherapy. More investigations will be needed to select ideal target to enhance the radio-sensitivity of glioma by use of the relationship.  
        关键词：Epidermal growth factor receptor (EGFR);Autophagy;Glioma;Radiation   
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        发布时间：2023-03-27
      • ZHANG Wei,ZHANG Jianhua,JIN Yan,WANG Shufeng,LIU Huarong,ZHANG Zhicheng
        Vol. 29, Issue 4, Pages: 202-208(2011)
        摘要：Polymer-nanoparticles composites with hollow structure were prepared by emulsion polymerization with Co3O4 nanoparticles (OA-NP) added into emulsion system containing styrene (St), sodium dodecyl sulfate (SDS) and cetyl alcohol (CA) under irradiation using γ-rays. Co3O4 nanoparticles were synthesized by hydrothermal method at first, and then modified with oleic acid (OA). The influence factors such as dosage of OA-NP and SDS on morphology and size distribution of hollow spheres were thoroughly studied. OA-NP was proved to inhibit the polymerization of St in the kinetic studies. It was found that pores occurred on hollow spheres’ shell at the first stage of reaction, and then the perfect shells formed gradually with polymerized time. The mechanism for the hollow spheres formation was proposed based on the obtained results. The soft magnetic properties of Co3O4 nanoparticles were influenced directly by polystyrene.  
        关键词：Cobalt oxide (II;III);Hollow sphere;Nanoparticles   
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        发布时间：2023-03-27
      • YANG Xuanxuan,DENG Bo,YU Ming,YU Yang,ZHANG Bowu,LI Jingye
        Vol. 29, Issue 4, Pages: 209-213(2011)
        摘要：Acryl amide (AAm) was grafted onto poly (vinylidene fluoride) (PVDF) powder by a γ-rays pre-irradiation induced graft polymerization technique. The DG values of the PVDF-g-PAM powder were determined by fluorine elemental analysis. Effects of grafting time on DG of PVDF-g-PAM powder at the same monomer concentration were studied. And modified powder was dissolved in NMP and added PVP as pore-forming agent. The microfiltration (MF) membranes were cast using a phase inversion method. The contact angle, degree of swelling, water flux and antifouling properties of those modified MF membranes were investigated. The results indicated that the hydrophilicity of modified MF membranes was improved obviously and the antifouling property of modified MF membranes (DG of 13%) was better than that of the pristine membrane.  
        关键词：Pre-irradiation induced graft polymerization;Hydrophilicity;Poly(vinylidene fluoride);Acryl amide;Microfiltration membranes   
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        发布时间：2023-03-27
      • SONG Hongtao,WEI Hongyuan,YANG Yuqing,WANG Guanquan,LUO Shunzhong
        Vol. 29, Issue 4, Pages: 214-218(2011)
        摘要：Peptide nucleic acid can be used in the field of tumor gene therapy with minimal non-target damage if it binds to gene with high sequence specificity and selectivity when labeled with radionuclides. In this paper, 99mTc (O) (PNA-dp) was synthesized, and the characteristics of DNA-cleavage were studied. It shows that the radiochemical purity remains over 90% after 6 h storage at room temperature, and the complex can be injected directly. Besides, the obvious DNA cleavage effect can be found after 1 h, and the mechanism of which maybe the cooperation of PNA-dp and the radiation of 99mTc.  
        关键词：99mTc;Peptide nucleic acid (PNA);DNA cleavage;pUC 019 DNA   
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        发布时间：2023-03-27
      • ZHANG Chunfang,YANG Wenge,XU Dalun,SHI Huidong
        Vol. 29, Issue 4, Pages: 219-225(2011)
        摘要：The influences of electron beam irradiation and package pattern (vacuum or ordinary) on the nutritional ingredient of Sciaenops ocellatus meat were investigated. The results were summarized as follows: (1) Electron-beam irradiation dose had notable effect on the moisture content, but no significant impact on the content of ash, protein, lipid and total carbohydrate. The package pattern had no significant effect on the common nutrional composition of Sciaenops ocellatus meat. (2) Either package pattern or irradiation dose showed little influence on the total amino acids, delicious amino acid, EAA/TAA and EAA/NEAA of Sciaenops ocellatus meat. The first limiting amino acid of Sciaenops ocellatus meat changed from Met plus Cys to Val in virtue of electron beam irradiation. (3) Both irradiation dose and package pattern showed no difference to the total fatty acid content. But the irradiation dose had notable effect on the relative content of unsaturated fatty acid, polyunsaturated fatty acid and DHA. The vacuum-packaged group had less DHA loss than the ordinary-packaged group with the same absorbed dose, and low dose groups had less DHA loss than the high groups. As conclusion, combining with the bactericidal effect of electron beam irradiation to sciaenops ocellatus meat, the recommended dose has been proposed to be 3-5 kGy.  
        关键词：Sciaenops ocellatus;Electron-beam radiation;Package;Nutritional ingredient   
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        发布时间：2023-03-27
      • HUANG Hong,WANG Naiyan,LIU Xitao,DOU Junfeng,HUANG Jianwei,DU Yongchao,LI Shuairan
        Vol. 29, Issue 4, Pages: 226-232(2011)
        摘要：To investigate mutagenic effects of polycyclic aromatic hydrocarbons (PAHs) degradation plant under phenanthrene stress and to test the germination level of alfalfa after ion-implantation alfalfa seeds were irradiated with N+ beam. The germination percentage and the root length distribution of alfalfa, which grew in environment with different mass fraction of phenanthrene was investigated, respectively. The results indicated that the relation of dose and germination rate was shown as "saddle" curve characteristic. It was found that the 5×1015 cm-2 would be the best dose of implantation. And the longest root length was about 10.32 cm. The experiment also showed phenanthrene would have inhibitive effect on germination percentage and root growth of alfalfa. The stress resistance of PAHs with alfalfa could be enhanced by ion implantation.  
        关键词：Ion implantation;Alfalfa;Polycyclic aromatic hydrocarbons   
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        发布时间：2023-03-27
      • WANG Jia,LI Yu,LI Mingjuan,MIN Rui
        Vol. 29, Issue 4, Pages: 233-237(2011)
        摘要：The changes of gelsolin in irradiated cells and its effects on oxidative injury of irradiated mice were investigated. The relative expression levels of cGSN in cultured human intestinal epithelial cells (HIEC) irradiated by 137Cs γ-rays with 8 Gy and 12 Gy were detected by western blot assay. BALB/c mice were divided randomly into gelsolin group treated with gelsolin, irradiated group and normal control group. Then the contents of plasma malonaldehyde (MDA), superoxide dismutase (SOD) and whole blood glutathione (GSH) were examined at 2 d and 7 d after irradiation of 6 Gy γ-rays. The relative level of cGSN in HIEC exposed to 8 Gy was lower than that of the normal level from 2 to 12 h post-radiation (the difference between post-irradiation observed at 12 h and normal level was significant, p<0.05), but higher than that of the normal level observed at 24 h post-radiation. The level of cGSN in HIEC exposed with 12 Gy was also lower than that of normal level determined at 2 h post-radiation but higher than that of the normal level from 4 to 24 h post-radiation. (The differences at 6 and 12 h time points comparing to the normal level were statistical significant respectively, p<0.05). Plasma levels of GSH and SOD in gelsolin treated mice after exposure to 6 Gy irradiation were obviously higher than that of irradiation treated groups at 2 and 7 d (p<0.05), whereas MDA level was significantly lower than that of only irradiation treated groups (p<0.05). Gelsolin might be able to protect mice against oxidative injury induced by radiation.  
        关键词：Gelsolin;Oxidative injury;Ionizing radiation;Superoxide dismutase;Malonaldehyde   
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        发布时间：2023-03-27
      • GENG Chong,CAO Jianping,NI Qianying,ZHU Wei,ZHANG Xuguang,CHEN Qiu,YANG Wei,ZHANG Shuyu,FENG Yang,ZHOU Yuanyuan,LUO Judong,CHEN Guanglie
        Vol. 29, Issue 4, Pages: 238-242(2011)
        摘要：To investigate the effect of artesunate on radio-sensitivity of human cervical cancer cells in vitro, The human cervical cancer cells HeLa and Siha were used as the experimental cells. MTT assay was used to determine the most appropriate drug concentration in the subsequent experiment, and the effect of human cervical cancer cells treated with artesunate and irradiation of 60Co γ-rays was studied by using conventional chromosomal aberration analysis and cytokinesis block method (CB method). The results show that when the concentration of artesunate in this experiment was 2.0 μmol/mL for HeLa cell and 4.0 μmol/mL for Siha cell respectively, the chromosome aberration, micronuclei cell and micronuclei rates of HeLa cells treated with artesunate were more serious than that of the only irradiation, but there is almost no change with Siha cells.  
        关键词：Artesunate;Human cervical cancer cells;Radiation sensitivity;Chromosomal mutation;Cytokinesis block method   
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        发布时间：2023-03-27
      • YANG Dan,XU Yujie,Bai Jie,WENG Wanweng,DING Wenxiu,HONG Chengjiao
        Vol. 29, Issue 4, Pages: 243-248(2011)
        摘要：The G2/M arrest of human oral epithelial carcinoma KB cells could be induced by the synergistic effect of ionizing radiation with paclitaxel, which results in the formation of multinucleated giant cells and eventually cell death. Earlier biochip screening analysis found that ten genes related to regulate cell division have been differential expression after treated with ionizing radiation and/or paclitaxel. This study was to investigate the potential molecular mechanism by quantitative assessment two gene expression levels among the ten genes. The SPATA5L1 gene was quantified by real-time quantitative PCR. And the protein expression levels of Cyclin B2 and SPATA5L1 were detected by Western Blot. The results show that the expression of SPATA5L1 gene increase after different doses irradiation (below 6 Gy) or irradiation combined with 40 nmol/L paclitaxel, however, the expression of Cyclin B2 decrease at the same time. We suppose that the expression of up-regulated SPATA5L1 and down-regulated Cyclin B2 maybe play a crucial role in the G2/M arrest and the formation of multinucleated giant cells, which block the cytoplasm split to form new daughter cell after nucleolus division.  
        关键词：Paclitaxel;KB cell;Multinucleated giant cell;SPATA5L1;Cyclin B2   
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        发布时间：2023-03-27
      • LI Yu,ZHOU Shiyan,HE Xingdong
        Vol. 29, Issue 4, Pages: 249-253(2011)
        摘要：The effect of sodium glycididazole (CMNa) on Zebrafish embryos was investigated. The wild-type Zebrafish eggs were placed in different concentrations of sodium glycididazole and received different doses of 60Co γ-rays irradiation. The morphological changes of embryonic development at different time and the survival rate were recorded at the 71 h. The repeat experiments and statistical analysis were further made. It was observed that the hatching rate of the normal form of the fish was 76%, 52%, 14% and 0% after exposure to γ-rays for 71 h with 0, 5, 10 and 15 Gy, respectively. And relative hatching rate was 40% and 0% after exposure to γ-rays with 5 and 10 Gy respectively when the samples treated with aqueous solution of 0.30 mmol/L CMNa. The radio-sensitive effects with treatment of 0.3 mmol/L CMNa was significantly higher than that in control (p<0.01). Zebrafish eggs may be suitable for the study of radio-sensitive effect as a special model. And it should take more attention as CMNa has a significant radio-sensitive effect on Zebrafish embryos.  
        关键词：Sodium glycididazole (CMNa);Zebrafish embryos;Radio-sensitivity   
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        发布时间：2023-03-27
      • JIAO Ling,ZHANG Wenyi,DING Yanqiu,QU Ximei,WANG Liqin,WANG Caixia,BAI Guilin
        Vol. 29, Issue 4, Pages: 254-256(2011)
        摘要：Four groups dog tooth enamel samples were irradiated with different dose by 137Cs γ-rays. Their electron spin resonance (ESR) spectra pre- and post-irradiated were measured and the changes of ESR signal intensities were analyzed. The results showed that the average intensity of native signals of dog tooth samples was 20.8±2.9, much weaker than that of human 39.5; the dosimetric signal intensities of dog tooth enamel increased with the absorbed doses; the mass of each sample is 100 mg. The average irradiation response of dog tooth enamel samples was (37.1±2.1) Gy-1, very close to that of human tooth samples (36.3 Gy-1). The dog tooth can be used for retrospective dosimetry when human tooth are difficult to collect.  
        关键词：γ-rays;Dog tooth enamel;Electron spin resonance (ESR);Dosimetry;Dose reconstruction   
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        发布时间：2023-03-27
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