最新刊期
      期 6 ， 2016

        Radiobiology and Radiomedicine

      • Dan TIAN,Yan WANG,Qin WANG,Liqing DU,Chang XU,Qiang LIU,Feiyue FAN
        Vol. 34, Issue 6, (2016) DOI: 10.11889/j.1000-3436.2016.rrj.34.060203
        摘要：To observe the effects of LGK-974 on radiosensitivity of HepG2 cells and explore its possible mechanism, MTT assay was applied to examine the growth inhibition of LGK-974 to HepG2; the radiosensitivity of HepG2 cells treated by 1.0 μmol/L LGK-974 was detected by clone formation assay; the reactive oxygen species level was examined by H2DCFH-DA probe flow cytometry method; the mRNA and protein levels of Nrf2, and the downstream gene HO-1, NQO-1 were accessed by RT-PCR and Western Blot, respectively. The results showed LGK-974 could inhibit HepG2 cells growth, and the IC50 of 24 h, 48 h and 72 h were 4.3 μmol/L, 2.14 μmol/L, and 0.536 μmol/L, respectively (p < 0.05); the SERD0 of 1.0 μmol/L LGK-974 was 1.37; LGK-974 could increase the ROS level produced by irradiation (p < 0.05); irradiation could increase the transcription and expression of Nrf2, HO-1, and NQO-1, and LGK-974 could weaken these effects. The study reveals that LGK-974 can enhance the radiosensitivity of HepG2 cells. The mechanism might inhibit the Nrf2 signaling pathway and prevent cells reducing the oxidative stress produced by irradiation.  
        关键词：LGK-974;HepG2 cells;Nrf2;Reactive oxygen species (ROS);Radiosensitization   
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        发布时间：2021-12-10
      • Langhuan LIU,Aiwu LUO,Keliang CHEN,Mengmeng GU,Beibei XIAO,Shitao ZOU,Chao HE,Jundong ZHOU,Bo HUANG
        Vol. 34, Issue 6, (2016) DOI: 10.11889/j.1000-3436.2016.rrj.34.060202
        摘要：Real-time PCR was used to examine the expression of FOXM1 (Forkhead box M1) in 40 esophageal squamous cell carcinoma (ESCC) tissues, and the adjacent normal tissues. Immunohistochemistry was used to examine the expression of FOXM1 protein in 94 ESCC tissues, and to analyze the relationship between ESCC and clinic pathologic. A small interfering RNA targeting FOXM1 was designed to silence the endogenous FOXM1 expression of ECA-109 and TE-1 cells. The effect of FOXM1 on radiation sensitive in esophageal squamous cell was observed by immunofluorescence stain, and colony formation assay. The results showed that FOXM1 mRNA expression level in esophageal cancer tissues was significantly higher than that of the adjacent tissues (p < 0.01), and the protein level expression of it has negative correlation with the survival times; the designed siRNA could dramatically silence FOXM1 expression in ECA-109 cells; γH2AX focus induced by X-rays in FOXM1 silent cells was significantly higher than that in control cells; the clone formation ability of the silent FOXM1 group was significantly lower than that in the control group. The content of FOXM1 is improved in ESCC, and is negatively correlated with the prognosis of patients; the cell radiation sensitive proliferation is significantly improved in the silent FOXM1 ECA-109 cells. In conclusion, FOXM1 has the potential to be the treatment of esophageal squamous carcinoma target.  
        关键词：Esophageal squamous cell carcinoma;FOXM1 poor prognosis;Radiation sensitivity;DNA damage repair   
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        发布时间：2021-12-10
      • Shuxia HAO,Huimin LYU,Chunyan WANG,Xuesong QI,Peng TONG,Qiao GOU
        Vol. 34, Issue 6, (2016) DOI: 10.11889/j.1000-3436.2016.rrj.34.060201
        摘要：In order to investigate the Anduolin injury prevention mechanism of microwave exposure, sexually matured SD rats were randomly divided into control group, exposure group, and prevention groups (drug dosage were 3, 6 and 9 mg/kg). Prevention groups were given drug continuously by oral administration for 20 d, meanwhile the control group and exposure group were given distilled water. After that the prevention groups and exposure group were exposed to high power-microwave (HPM) of 100 mW/cm2 for 10 min. Rats were killed at 1st, 2nd, and 5th day after exposure, and the pathological specimens were prepared from testicular tissue. Interested proteins in testicular tissue were detected with immunohistochemical method, and the protein content were analyzed with image-pro plus analysis software. The results showed that protein content of Caspase-9 of exposure group showed a high-low-high trend compared with control group (p < 0.05) at 1st, 2nd, and 5th day after exposed to HPM, while the prevention groups showed a trend from low to high with time (p < 0.05); for X-linked inhibitor of apoptosis protein (XIAP) protein, there was no significantly difference between exposure group and control group, while the XIAP levels of 6 mg/kg group and 9 mg/kg group at 5th day after exposure increased significantly compared with the exposure group (p < 0.05). The results suggest that Caspase-9 is involved in the HPM-induced apoptosis in rat testis, and protective effect of Anduolin on rat testicular cells requires participation of Caspase-9 and XIAP.  
        关键词：High power microwave;Anduolin;Apoptosis;Caspase-9;X-linked inhibitor of apoptosis protein (XIAP)   
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        发布时间：2021-12-10

        Radiation Chemistry

      • Luyu GU,Jianxi LI,Hu LIU,Wanxin LI
        Vol. 34, Issue 6, (2016) DOI: 10.11889/j.1000-3436.2016.rrj.34.060301
        摘要：Ethylene-vinyl acetate copolymer (EVA) was blended with different contents of triallyl isocyanurate (TAIC) to prepare halogen-free flame retardant heat shrinkable material by the electron beam irradiation crosslinking. Effects of TAIC content and absorbed dose on gel content, mechanical properties, and electrical properties of samples were studied. And the EVA heat shrinkable materials were characterized by the Fourier transform infrared (FTIR) spectroscopy. The results showed that the gel content of EVA heat shrinkable material increased with the TAIC content in the case of absorbed dose remaining unchanged, e.g. 150 kGy, and the gel content increased with the absorbed dose while adding certain TAIC, e.g. 3 phr (parts per hundred as for mass), which indicated that TAIC could promote the role of irradiation crosslinking. The elongation at break decreased with the icreasing TAIC contents and the increasing absorbed doses. However, the tensile strength of the samples increased with the TAIC content. Test results of volume resistance showed that with TAIC contents increasing, volume resistance of EVA heat shrinkable materials increased at first, and then decreased with the increasing absorbed doses. The FTIR analysis showed that the TAIC reaction was complete under the electron beam irradiation.  
        关键词：Ethylene vinyl acetate copolymer (EVA);Triallyl isocyanurate (TAIC);Irradiation crosslinking;Electrical properties;Infrared analysis   
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        发布时间：2021-12-10

        Radiation Technology Application

      • Yong CHENG,Xiaohu LI,Lulu ZHOU,Jiaqiang XU,Wenfa ZHANG,Wenli ZHANG,Weihua LIU,Rongfang SHEN,Mouhua WANG
        Vol. 34, Issue 6, (2016) DOI: 10.11889/j.1000-3436.2016.rrj.34.060401
        摘要：Polycarbosilane (PCS) was irradiated by γ-rays in N2 atmosphere. The chemical structure, molecular weight, thermal decomposition characteristics of the irradiated PCS were investigated by Fourier transform infrared spectroscopic analysis, gel permeation chromatographic analysis and thermogravimetric analysis, respectively. The results showed that a slight weight loss of PCS was found after γ-rays irradiation, both the molecular weight and softening point of PCS increased with the absorbed dose. These results indicated that crosslinking reactions occurred between PCS molecules induced by γ-rays irradiation. According to the results by Fourier transform infrared spectroscopic analysis, the crosslinking site of PCS was Si-C-Si structure which was formed by combining of Si-H with C-H structure. The ceramic yield of irradiated PCS increased significantly with its absorbed dose. When the absorbed dose reached 1.5 MGy, the ceramic yield increased from 61.9% of non-irradiated sample to a saturated value of 80.0%.  
        关键词：Polycarbosilane;γ-rays irradiation;Crosslinking;Ceramic yield   
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        发布时间：2021-12-10

        Radiation Interdisciplinary Research

      • Zequn ZHANG,Xiaodan HU,Wei ZHANG,Guocai LIU,Shuyue YU,Haiqian ZHANG
        Vol. 34, Issue 6, (2016) DOI: 10.11889/j.1000-3436.2016.rrj.34.060701
        摘要：Gadolinium doped calcium fluoride (CaF2:Gd) nanoparticles were prepared by the hytrothermal method. X-ray diffraction (XRD), Scanning electron microscope (SEM), and Energy dispersive spectrometer (EDS) were used to characterize the nanomaterials and obtained the 44 nm average particle size, the cubic crystal and element compose (Ca, F, Gd). The combination of Tm-Tstop and CGCD (Computerized glow curves de-convolved) were used and four overlapping peaks were found in glow curve at 126, 148, 174 and 286℃. And the kinetic parameters of the synthesized nanomaterials were also obtained. The optimal Gd mole percent was found at 0.6%. A good linear dose response was shown from 1 Gy to 10 000 Gy. The fading and repeatability of the nanoparticles were also excellent. It can be concluded that the CaF2:Gd nanomaterials could be employed to measure the high dose gamma radiation.  
        关键词：Thermoluminescence;Hytrothermal;Gadolinium doped calcium fluoride;Nanomaterials   
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        发布时间：2021-12-10

        Review

      • Yuan SONG,Jing LIU,Jihong CHEN,Wenjian LI,Wei HU,Lu LIU
        Vol. 34, Issue 6, (2016) DOI: 10.11889/j.1000-3436.2016.rrj.34.06.01.01
        摘要：The progress of fungal melanins' radioprotection mechanism was summarized in four aspects:electron transfer and energy transmission, radicals scavenging, binding of metals, and photoprotection. The breakthrough in the future research will be focused on the determination of secondary structure, the energy transfer effect, and the radioprotection in organism.  
        关键词：Melanin;Fungi;Radiation protection   
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        发布时间：2021-12-10
      • Ping SONG,Fawei XIANG,Min LI,Lihua WANG,Xiaolei ZUO
        Vol. 34, Issue 6, (2016) DOI: 10.11889/j.1000-3436.2016.rrj.34.060102
        摘要：We summary the models of DNA allosteric control and possible effects (pH value, DNA effector, ion, radio) of DNA dynamic allosteric. And a bio-medical possible application of DNA allosteric is proposed.  
        关键词：Allosteric;Hill coefficient;Dynamic range;Review   
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        发布时间：2022-09-30
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