最新刊期
期 2 ， 2017
- 摘要：This review summarizes the sequential advancements made in research involving graphene and its derivatives, including their modification, structure design, and applications. Further, the review discusses the preparation of graphene-based functional materials from liquid and solid reaction systems by gamma-ray irradiation, which induces different chemical effects. Lastly, the potential application of the gamma-ray irradiation technology in the research on graphene-based functional materials is also proposed.关键词：Graphene;Functional materials;Gamma-ray;Irradiation technology
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发布时间：2021-12-10
REVIEW
- 摘要：The Cell Counting Kit-8 (CCK-8) was used to detect the cell survival rate of HaCaT cells. The apoptosis rate and mortality of HaCaT cells were detected using flow cytometry with the Annexin VFITC and PI double labeling method. Autophagosomes and the generation of reactive oxygen species (ROS) in HaCaT cells were monitored by laser scanning confocal microscopy using monodansylcadaverine (MDC) and dichlorodihydrofluorescein diacetate (DCFH-DA) staining, respectively. The expression of LC3B-II, p-AKT, and total AKT protein in HaCaT cells was determined by western blotting. The results showed that HaCaT cell proliferation was inhibited gradually (p < 0.05) after irradiation with a dose of 10~80 J/cm2 UVA. Treatment with resveratrol enhanced the proliferation of UVA-irradiated HaCaT cells (p < 0.01) and decreased their apoptosis rate and mortality (p < 0.05). The expression of LC3B-II protein was increased (p < 0.01) after irradiation with 20 J/cm2 UVA for 3~24 h. After treatment with resveratrol, autophagy was enhanced (p < 0.01), intracellular ROS level was decreased (p < 0.01), and p-AKT expression was significantly increased (p < 0.01) in UVA-irradiated HaCaT cells. These results indicate that resveratrol could reduce UVA-induced proliferative inhibition, apoptosis, and death in HaCaT cells by enhancing the autophagic process and p-AKT protein expression and by reducing ROS production.关键词：Resveratrol;HaCaT cells;UVA;Protective effect
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发布时间：2021-12-10 - 摘要：CAL-27, a human tongue squamous carcinoma cell line was selected for the experiment. The cell clone formation rate was detected by a cell clone forming experiment and flow cytometry was used to detect apoptosis. High throughput sequencing technique (HiSeq) was applied for detecting the differentially expressed miRNAs (DEGs) after irradiating CAL-27 cells with X-rays. Using bioinformatics analysis, the distribution of candidate target genes and significantly enriched GO-term genes were obtained. The main signaling pathways involving the DEGs were obtained using KEGG pathway enrichment analysis. The CAL-27 cell clone survival rate of the 2 Gy irradiated group was 59% and the apoptosis rate was 47.5% (p < 0.05). After irradiation, 21 miRNAs were altered, of which 18 miRNAs were upregulated (hsa-miR-21-3p, hsa-miR-101-3p, hsa-miR-100-3p, etc.) and 3 miRNAs were downregulated (hsa-miR-204-5p, hsa-miR-10a-5p, hsa-miR-146b-5p). When analyzed by GO function, there were 23 GO-terms enriched in biological process, 20 GO-terms enriched in molecular function, and 18 GO-terms enriched in cellular component. Furthermore, 18 KEGG pathways in which the DEGs participated, were screened out including olfactory transduction, cytokine-cytokine receptor interaction, MAPK signaling pathway-yeast (p < 0.05), etc. These results indicate that X-ray irradiation with a dose of 2 Gy altered the miRNA expression profile of CAL-27 cells, and the underlying mechanism may involve MAPK signaling pathways. This study lays a foundation for further study of miRNAs in the molecular mechanism of tongue squamous carcinoma radiotherapy.关键词：X-ray irradiation;CAL-27 cell;MicroRNA
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发布时间：2021-12-10 - 摘要：To identify the key differentially expressed long non-coding RNAs in irradiated lung carcinoma A549 cells by bioinformatics analysis, the mRNA microarray dataset GSE24876 was downloaded from the Gene Expression Omnibus database and analyzed by GEO2R to obtain differentially expressed gene probes. Re-annotation of probes was performed for the differentially expressed genes using DAVID. In total, 33 differentially expressed lncRNAs were identified from GSE24876, which included 4 upregulated lncRNAs and 29 downregulated lncRNAs in irradiated A549 cells compared with non-irradiated A549 cells. The Gene-E software was then used to confirm the expression levels of the lncRNAs and the results were similar to those analyzed by GEO2R. Among them, high expression of LINC00319 (HR 1.21 [1.02~1.42], p=0.026) and LINC01095 (HR 1.18 [1.01~1.40], p=0.042) was associated with worse overall survival in lung cancer patients. However, further studies are needed to verify the results of this study.关键词：Long non-coding RNA;Lung carcinoma;Irradiation;Gene microarray;Bioinformatics analysis
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发布时间：2021-12-10
RADIOBIOLOGY AND RADIOMEDICINE
- 摘要：MG-6600 and citric acid were used as a water and oil repellent and as an anti-wrinkle agent, respectively. Cotton fabrics were subjected to one of the following three processes: (1) the pad-dry-cure process, (2) electron beam irradiation grafting, and (3) plasma pretreatment, followed by electron beam irradiation grafting. Fourier transform infrared spectroscopy (FTIR) proved that MG-6600 and citric acid were successfully grafted onto the cotton fiber. Scanning electron microscopy showed that the surfaces of the three types of treated cotton fabrics were covered by layers. Further, longitudinal stripes appeared on the surface of the fabric subjected to plasma pre-treatment. The change in the cotton fabric properties was characterized by measuring the wrinkle recovery angle (WRA), the contact angle (WCA), and the contact angle to oil (n-hexadecane) (OCA). The results showed that the wrinkle resistance and the water and oil repellency were optimized when the fabric was subjected to plasma pretreatment and electron beam irradiation: the WRA, WCA, and OCA were 185.9°, 158.9°, and 129.1°. Further, the breaking strength and whiteness of the cotton fabric met the requirements of the apparel standards, and the washing durability of cotton fabric was significantly enhanced.关键词：Cotton fabric;Electron beam irradiation grafting;Plasma pre-treatment;Anti-wrinkle finishing;Water and oil repellent finishing
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发布时间：2021-12-10 - 摘要：The ethanol-producing yeast Saccharomyces cerevisiae was induced by N+ implantation with an output power of 18 keV and a dose of 400×1014 cm-2, depending on the survival and mutation rates. The UV exposure time was 60 s. Under optimized conditions, the strain achieved the highest positive mutation and lethality rates. A mutant strain SC-N9015 was selected; the ethanol yield of this strain was 15.53%, whereas that of the parental strain was 14.65%. Further, evaluation of ethanol yield over successive generations indicated the hereditary stability of the mutations. The study shows that the combination of low energy N+ implantation with UV rays is an efficient method for mutation breeding of S. cerevisiae.关键词：Saccharomyces cerevisiae;Low energy N+ implantation;UV rays;Irradiation;Mutation breeding
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发布时间：2021-12-10 - 摘要：The feasibility and detailed methods for personal dose monitoring and assessment in non-uniform radiation fields have not been included in national standards. A multi-dosimetry system was designed and developed to obtain a more accurate personal dose assessment in a non-uniform radiation field. The system's design was based on the American standards and incorporated in the Chinese actual environment. The system was tested during a nuclear power plant outage. The test results showed that the different components of the body dose distribution of the exposed personnel can be obtained by using of the multi-dosimetry system, and the accuracy of the effective dose estimation can be improved. It can also help to improve the quality of collective and individual dose control management.关键词：Non-uniform radiation field;Personal dose monitoring and assessment;Multi-dosimetry system
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发布时间：2021-12-10
RADIATION CHEMISTRY
- 摘要：The narrow space in a railcar body increase its thermal resistance and also enable improving the thermal resistance of the railcar body based on the radiation principle. In this study, the improvement of the thermal resistance by pasting aluminum foils on the inner surfaces of the enclosure has been tested. The results show that the heat radiation in the enclosure cannot be neglected because the amount of radiated heat is very large. An effective method to reduce the heat radiation in the enclosure involves pasting aluminum foils on the inner surfaces, and the effect becomes increasingly obvious, with up to 62% reduction in the radiated heat, as the enclosure thickness. Further, the improvement in the enclosure thermal resistance by pasting aluminum foils on one of the inner surfaces is almost the same whether on the cold side or the hot side, and the improvement when both inner surfaces are covered with aluminum foils is about 5% better than that when one surface is covered.关键词：Narrow enclosure;Heat radiation;Improving thermal resistance;Equivalent thermal conductivity
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发布时间：2021-12-10
RADIATION PHYSICS
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